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. 1997 Apr-Jun;3(2):213–221. doi: 10.3201/eid0302.970219

Brucellosis: an overview.

M J Corbel 1
PMCID: PMC2627605  PMID: 9204307

Abstract

Brucellosis remains a major zoonosis worldwide. Although many countries have eradicated Brucella abortus from cattle, in some areas Brucella melitensis has emerged as a cause of infection in this species as well as in sheep and goats. Despite vaccination campaigns with the Rev 1 strain, B. melitensis remains the principal cause of human brucellosis. Brucella suis is also emerging as an agent of infection in cattle, thus extending its opportunities to infect humans. The recent isolation of distinctive strains of Brucella from marine mammals has extended its ecologic range. Molecular genetic studies have demonstrated phylogenetic affiliation to Agrobacterium, Phyllobacterium, Ochrobactrum, and Rhizobium. Polymerase chain reaction and gene probe development may provide more effective typing methods. Pathogenicity is related to production of lipopolysaccharides containing a poly N-formyl perosamine O chain, CuZn superoxide dismutase, erythrlose phosphate dehydrogenase, stress-induced proteins related to intracellular survival, and adenine and guanine monophosphate inhibitors of phagocyte functions. Protective immunity is conferred by antibody to lipopolysaccharide and T-cell-mediated macrophage activation triggered by protein antigens. Diagnosis still centers on isolation of the organism and serologic test results, especially enzyme immunoassay, which is replacing other methods. Polymerase chain reaction is also under evaluation. Therapy is based on tetracyclines with or without rifampicin, aminoglycosides, or quinolones. No satisfactory vaccines against human brucellosis are available, although attenuated purE mutants appear promising.

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Selected References

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  1. Akova M., Uzun O., Akalin H. E., Hayran M., Unal S., Gür D. Quinolones in treatment of human brucellosis: comparative trial of ofloxacin-rifampin versus doxycycline-rifampin. Antimicrob Agents Chemother. 1993 Sep;37(9):1831–1834. doi: 10.1128/aac.37.9.1831. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Allardet-Servent A., Bourg G., Ramuz M., Pages M., Bellis M., Roizes G. DNA polymorphism in strains of the genus Brucella. J Bacteriol. 1988 Oct;170(10):4603–4607. doi: 10.1128/jb.170.10.4603-4607.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Araj G. F., Lulu A. R., Mustafa M. Y., Khateeb M. I. Evaluation of ELISA in the diagnosis of acute and chronic brucellosis in human beings. J Hyg (Lond) 1986 Dec;97(3):457–469. doi: 10.1017/s0022172400063634. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ariza J., Gudiol F., Pallarés R., Rufí G., Fernández-Viladrich P. Comparative trial of rifampin-doxycycline versus tetracycline-streptomycin in the therapy of human brucellosis. Antimicrob Agents Chemother. 1985 Oct;28(4):548–551. doi: 10.1128/aac.28.4.548. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Ariza J., Pellicer T., Pallarés R., Foz A., Gudiol F. Specific antibody profile in human brucellosis. Clin Infect Dis. 1992 Jan;14(1):131–140. doi: 10.1093/clinids/14.1.131. [DOI] [PubMed] [Google Scholar]
  6. Bachrach G., Banai M., Bardenstein S., Hoida G., Genizi A., Bercovier H. Brucella ribosomal protein L7/L12 is a major component in the antigenicity of brucellin INRA for delayed-type hypersensitivity in brucella-sensitized guinea pigs. Infect Immun. 1994 Dec;62(12):5361–5366. doi: 10.1128/iai.62.12.5361-5366.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Banai M., Mayer I., Cohen A. Isolation, identification, and characterization in Israel of Brucella melitensis biovar 1 atypical strains susceptible to dyes and penicillin, indicating the evolution of a new variant. J Clin Microbiol. 1990 May;28(5):1057–1059. doi: 10.1128/jcm.28.5.1057-1059.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bundle D. R., Cherwonogrodzky J. W., Gidney M. A., Meikle P. J., Perry M. B., Peters T. Definition of Brucella A and M epitopes by monoclonal typing reagents and synthetic oligosaccharides. Infect Immun. 1989 Sep;57(9):2829–2836. doi: 10.1128/iai.57.9.2829-2836.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Canning P. C., Roth J. A., Deyoe B. L. Release of 5'-guanosine monophosphate and adenine by Brucella abortus and their role in the intracellular survival of the bacteria. J Infect Dis. 1986 Sep;154(3):464–470. doi: 10.1093/infdis/154.3.464. [DOI] [PubMed] [Google Scholar]
  10. Caron E., Peyrard T., Köhler S., Cabane S., Liautard J. P., Dornand J. Live Brucella spp. fail to induce tumor necrosis factor alpha excretion upon infection of U937-derived phagocytes. Infect Immun. 1994 Dec;62(12):5267–5274. doi: 10.1128/iai.62.12.5267-5274.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Cellier M. F., Teyssier J., Nicolas M., Liautard J. P., Marti J., Sri Widada J. Cloning and characterization of the Brucella ovis heat shock protein DnaK functionally expressed in Escherichia coli. J Bacteriol. 1992 Dec;174(24):8036–8042. doi: 10.1128/jb.174.24.8036-8042.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Cieslak T. J., Robb M. L., Drabick C. J., Fischer G. W. Catheter-associated sepsis caused by Ochrobactrum anthropi: report of a case and review of related nonfermentative bacteria. Clin Infect Dis. 1992 Apr;14(4):902–907. doi: 10.1093/clinids/14.4.902. [DOI] [PubMed] [Google Scholar]
  13. Cloeckaert A., Verger J. M., Grayon M., Grépinet O. Restriction site polymorphism of the genes encoding the major 25 kDa and 36 kDa outer-membrane proteins of Brucella. Microbiology. 1995 Sep;141(Pt 9):2111–2121. doi: 10.1099/13500872-141-9-2111. [DOI] [PubMed] [Google Scholar]
  14. Corbel M. J. Identification of dye-sensitive strains of Brucella melitensis. J Clin Microbiol. 1991 May;29(5):1066–1068. doi: 10.1128/jcm.29.5.1066-1068.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Corbel M. J. The immunogenic activity of ribosomal fractions derived from Brucella abortus. J Hyg (Lond) 1976 Feb;76(1):65–74. doi: 10.1017/s0022172400054954. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Corbel M. J., Thomas E. L. Use of phage for the identification of Brucella canis and Brucella ovis cultures. Res Vet Sci. 1985 Jan;38(1):35–40. [PubMed] [Google Scholar]
  17. Corbel M. J. Vaccines against bacterial zoonoses. J Med Microbiol. 1997 Apr;46(4):267–269. doi: 10.1099/00222615-46-4-267. [DOI] [PubMed] [Google Scholar]
  18. Crawford R. M., Van De Verg L., Yuan L., Hadfield T. L., Warren R. L., Drazek E. S., Houng H. H., Hammack C., Sasala K., Polsinelli T. Deletion of purE attenuates Brucella melitensis infection in mice. Infect Immun. 1996 Jun;64(6):2188–2192. doi: 10.1128/iai.64.6.2188-2192.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Da Costa M., Guillou J. P., Garin-Bastuji B., Thiébaud M., Dubray G. Specificity of six gene sequences for the detection of the genus Brucella by DNA amplification. J Appl Bacteriol. 1996 Sep;81(3):267–275. doi: 10.1111/j.1365-2672.1996.tb04328.x. [DOI] [PubMed] [Google Scholar]
  20. Douglas J. T., Rosenberg E. Y., Nikaido H., Verstreate D. R., Winter A. J. Porins of Brucella species. Infect Immun. 1984 Apr;44(1):16–21. doi: 10.1128/iai.44.1.16-21.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ewalt D. R., Payeur J. B., Martin B. M., Cummins D. R., Miller W. G. Characteristics of a Brucella species from a bottlenose dolphin (Tursiops truncatus). J Vet Diagn Invest. 1994 Oct;6(4):448–452. doi: 10.1177/104063879400600408. [DOI] [PubMed] [Google Scholar]
  22. Ficht T. A., Bearden S. W., Sowa B. A., Adams L. G. DNA sequence and expression of the 36-kilodalton outer membrane protein gene of Brucella abortus. Infect Immun. 1989 Nov;57(11):3281–3291. doi: 10.1128/iai.57.11.3281-3291.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ficht T. A., Husseinen H. S., Derr J., Bearden S. W. Species-specific sequences at the omp2 locus of Brucella type strains. Int J Syst Bacteriol. 1996 Jan;46(1):329–331. doi: 10.1099/00207713-46-1-329. [DOI] [PubMed] [Google Scholar]
  24. Goldbaum F. A., Leoni J., Wallach J. C., Fossati C. A. Characterization of an 18-kilodalton Brucella cytoplasmic protein which appears to be a serological marker of active infection of both human and bovine brucellosis. J Clin Microbiol. 1993 Aug;31(8):2141–2145. doi: 10.1128/jcm.31.8.2141-2145.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Goldbaum F. A., Morelli L., Wallach J., Rubbi C. P., Fossati C. A. Human brucellosis: immunoblotting analysis of three Brucella abortus antigenic fractions allows the detection of components of diagnostic importance. Medicina (B Aires) 1991;51(3):227–232. [PubMed] [Google Scholar]
  26. Jumas-Bilak E., Maugard C., Michaux-Charachon S., Allardet-Servent A., Perrin A., O'Callaghan D., Ramuz M. Study of the organization of the genomes of Escherichia coli, Brucella melitensis and Agrobacterium tumefaciens by insertion of a unique restriction site. Microbiology. 1995 Oct;141(Pt 10):2425–2432. doi: 10.1099/13500872-141-10-2425. [DOI] [PubMed] [Google Scholar]
  27. Khuri-Bulos N. A., Daoud A. H., Azab S. M. Treatment of childhood brucellosis: results of a prospective trial on 113 children. Pediatr Infect Dis J. 1993 May;12(5):377–381. doi: 10.1097/00006454-199305000-00005. [DOI] [PubMed] [Google Scholar]
  28. Kolman S., Maayan M. C., Gotesman G., Rozenszajn L. A., Wolach B., Lang R. Comparison of the Bactec and lysis concentration methods for recovery of Brucella species from clinical specimens. Eur J Clin Microbiol Infect Dis. 1991 Aug;10(8):647–648. doi: 10.1007/BF01975817. [DOI] [PubMed] [Google Scholar]
  29. Lin J., Ficht T. A. Protein synthesis in Brucella abortus induced during macrophage infection. Infect Immun. 1995 Apr;63(4):1409–1414. doi: 10.1128/iai.63.4.1409-1414.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Luzzi G. A., Brindle R., Sockett P. N., Solera J., Klenerman P., Warrell D. A. Brucellosis: imported and laboratory-acquired cases, and an overview of treatment trials. Trans R Soc Trop Med Hyg. 1993 Mar-Apr;87(2):138–141. doi: 10.1016/0035-9203(93)90460-8. [DOI] [PubMed] [Google Scholar]
  31. Mantur B. G., Mangalgi S. S., Mulimani M. Brucella melitensis--a sexually transmissible agent? Lancet. 1996 Jun 22;347(9017):1763–1763. doi: 10.1016/s0140-6736(96)90839-9. [DOI] [PubMed] [Google Scholar]
  32. Matar G. M., Khneisser I. A., Abdelnoor A. M. Rapid laboratory confirmation of human brucellosis by PCR analysis of a target sequence on the 31-kilodalton Brucella antigen DNA. J Clin Microbiol. 1996 Feb;34(2):477–478. doi: 10.1128/jcm.34.2.477-478.1996. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Michaux S., Paillisson J., Carles-Nurit M. J., Bourg G., Allardet-Servent A., Ramuz M. Presence of two independent chromosomes in the Brucella melitensis 16M genome. J Bacteriol. 1993 Feb;175(3):701–705. doi: 10.1128/jb.175.3.701-705.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  34. Minnick M. F., Stiegler G. L. Nucleotide sequence and comparison of the 5S ribosomal RNA genes of Rochalimaea henselae, R.quintana and Brucella abortus. Nucleic Acids Res. 1993 May 25;21(10):2518–2518. doi: 10.1093/nar/21.10.2518. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Mustafa A. A., Abusowa M. Field-oriented trial of the Chinese Brucella suis strain 2 vaccine on sheep and goats in Libya. Vet Res. 1993;24(5):422–429. [PubMed] [Google Scholar]
  36. O'Hara M. J., Collins D. M., de Lisle G. W. Restriction endonuclease analysis of Brucella ovis and other Brucella species. Vet Microbiol. 1985 Aug;10(5):425–429. doi: 10.1016/0378-1135(85)90024-0. [DOI] [PubMed] [Google Scholar]
  37. Oliveira S. C., Splitter G. A. Immunization of mice with recombinant L7/L12 ribosomal protein confers protection against Brucella abortus infection. Vaccine. 1996 Jul;14(10):959–962. doi: 10.1016/0264-410x(96)00018-7. [DOI] [PubMed] [Google Scholar]
  38. Potasman I., Even L., Banai M., Cohen E., Angel D., Jaffe M. Brucellosis: an unusual diagnosis for a seronegative patient with abscesses, osteomyelitis, and ulcerative colitis. Rev Infect Dis. 1991 Nov-Dec;13(6):1039–1042. doi: 10.1093/clinids/13.6.1039. [DOI] [PubMed] [Google Scholar]
  39. Relman D. A., Lepp P. W., Sadler K. N., Schmidt T. M. Phylogenetic relationships among the agent of bacillary angiomatosis, Bartonella bacilliformis, and other alpha-proteobacteria. Mol Microbiol. 1992 Jul;6(13):1801–1807. doi: 10.1111/j.1365-2958.1992.tb01352.x. [DOI] [PubMed] [Google Scholar]
  40. Rigby C. E., Fraser A. D. Plasmid transfer and plasmid-mediated genetic exchange in Brucella abortus. Can J Vet Res. 1989 Jul;53(3):326–330. [PMC free article] [PubMed] [Google Scholar]
  41. Ross H. M., Foster G., Reid R. J., Jahans K. L., MacMillan A. P. Brucella species infection in sea-mammals. Vet Rec. 1994 Apr 2;134(14):359–359. doi: 10.1136/vr.134.14.359-b. [DOI] [PubMed] [Google Scholar]
  42. Sangari F. J., García-Lobo J. M., Agüero J. The Brucella abortus vaccine strain B19 carries a deletion in the erythritol catabolic genes. FEMS Microbiol Lett. 1994 Sep 1;121(3):337–342. doi: 10.1111/j.1574-6968.1994.tb07123.x. [DOI] [PubMed] [Google Scholar]
  43. Santini C., Baiocchi P., Berardelli A., Venditti M., Serra P. A case of brain abscess due to Brucella melitensis. Clin Infect Dis. 1994 Nov;19(5):977–978. doi: 10.1093/clinids/19.5.977. [DOI] [PubMed] [Google Scholar]
  44. Schurig G. G., Roop R. M., 2nd, Bagchi T., Boyle S., Buhrman D., Sriranganathan N. Biological properties of RB51; a stable rough strain of Brucella abortus. Vet Microbiol. 1991 Jul;28(2):171–188. doi: 10.1016/0378-1135(91)90091-s. [DOI] [PubMed] [Google Scholar]
  45. Shakir R. A., Al-Din A. S., Araj G. F., Lulu A. R., Mousa A. R., Saadah M. A. Clinical categories of neurobrucellosis. A report on 19 cases. Brain. 1987 Feb;110(Pt 1):213–223. doi: 10.1093/brain/110.1.213. [DOI] [PubMed] [Google Scholar]
  46. Shakir R. A. Neurobrucellosis. Postgrad Med J. 1986 Dec;62(734):1077–1079. doi: 10.1136/pgmj.62.734.1077. [DOI] [PMC free article] [PubMed] [Google Scholar]
  47. Tatum F. M., Cheville N. F., Morfitt D. Cloning, characterization and construction of htrA and htrA-like mutants of Brucella abortus and their survival in BALB/c mice. Microb Pathog. 1994 Jul;17(1):23–36. doi: 10.1006/mpat.1994.1049. [DOI] [PubMed] [Google Scholar]
  48. Tatum F. M., Morfitt D. C., Halling S. M. Construction of a Brucella abortus RecA mutant and its survival in mice. Microb Pathog. 1993 Mar;14(3):177–185. doi: 10.1006/mpat.1993.1018. [DOI] [PubMed] [Google Scholar]
  49. Tessaro S. V., Forbes L. B. Brucella suis biotype 4: a case of granulomatous nephritis in a barren ground caribou (Rangifer tarandus groenlandicus L.) with a review of the distribution of rangiferine brucellosis in Canada. J Wildl Dis. 1986 Oct;22(4):479–483. doi: 10.7589/0090-3558-22.4.479. [DOI] [PubMed] [Google Scholar]
  50. Young E. J. An overview of human brucellosis. Clin Infect Dis. 1995 Aug;21(2):283–290. doi: 10.1093/clinids/21.2.283. [DOI] [PubMed] [Google Scholar]
  51. Zhan Y., Kelso A., Cheers C. Differential activation of Brucella-reactive CD4+ T cells by Brucella infection or immunization with antigenic extracts. Infect Immun. 1995 Mar;63(3):969–975. doi: 10.1128/iai.63.3.969-975.1995. [DOI] [PMC free article] [PubMed] [Google Scholar]

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