Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1993 Feb;31(2):354–358. doi: 10.1128/jcm.31.2.354-358.1993

Two-way cross-neutralization mediated by a shared P (VP4) serotype between bovine rotavirus strains with distinct G (VP7) serotypes.

Y Matsuda 1, Y Isegawa 1, G N Woode 1, S Zheng 1, E Kaga 1, T Nakagomi 1, S Ueda 1, O Nakagomi 1
PMCID: PMC262764  PMID: 8381812

Abstract

Among bovine rotavirus strains, there are three G serotypes (G6, G8, and G10) and three P (VP4) serotypes (PB1, PB2, and PB3, which are defined on the basis of strains NCDV, UK, and B223, respectively). Plaque reduction neutralization assays with hyperimmune antisera disclosed two-way antigenic relationships of strain KN-4 with strain KK-3 (G10, PB3) as well as with strains NCDV (G6, PB1) and 0510 (G6, PB2). Neutralization assays with monoclonal antibodies specific for G6, G10, and PB3 revealed that KK-3 and KN-4 had the same P serotype (PB3) but that neither G6- nor G10-specific monoclonal antibody neutralized KN-4. Comparison of the VP7 gene sequence of KN-4 with those of other bovine rotavirus strains indicated that KN-4 was more similar to G6 bovine strains than to KK-3 and other G10 strains, suggesting that the G serotype of KN-4 was G6. From these results, we concluded that the two-way cross-neutralization between KN-4 and NCDV or 0510 was mediated by shared G6 serotype specificity, whereas the two-way cross-neutralization between KN-4 and KK-3 was mediated by shared P serotype specificity (PB3). Thus, KN-4 and KK-3 represent the first reported example of a two-way antigenic relationship mediated only by the P serotype. This article emphasizes the need for adopting a binary serotyping system and development of reagents which will enable classification of rotaviruses based on their G and P serotype specificities.

Full text

PDF
354

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Browning G. F., Chalmers R. M., Fitzgerald T. A., Snodgrass D. R. Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype. J Gen Virol. 1991 May;72(Pt 5):1059–1064. doi: 10.1099/0022-1317-72-5-1059. [DOI] [PubMed] [Google Scholar]
  2. Browning G. F., Fitzgerald T. A., Chalmers R. M., Snodgrass D. R. A novel group A rotavirus G serotype: serological and genomic characterization of equine isolate FI23. J Clin Microbiol. 1991 Sep;29(9):2043–2046. doi: 10.1128/jcm.29.9.2043-2046.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Clark H. F., Hoshino Y., Bell L. M., Groff J., Hess G., Bachman P., Offit P. A. Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol. 1987 Sep;25(9):1757–1762. doi: 10.1128/jcm.25.9.1757-1762.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dyall-Smith M. L., Lazdins I., Tregear G. W., Holmes I. H. Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proc Natl Acad Sci U S A. 1986 May;83(10):3465–3468. doi: 10.1073/pnas.83.10.3465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Elleman T. C., Hoyne P. A., Dyall-Smith M. L., Holmes I. H., Azad A. A. Nucleotide sequence of the gene encoding the serotype-specific glycoprotein of UK bovine rotavirus. Nucleic Acids Res. 1983 Jul 25;11(14):4689–4701. doi: 10.1093/nar/11.14.4689. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Estes M. K., Cohen J. Rotavirus gene structure and function. Microbiol Rev. 1989 Dec;53(4):410–449. doi: 10.1128/mr.53.4.410-449.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Glass R. I., Keith J., Nakagomi O., Nakagomi T., Askaa J., Kapikian A. Z., Chanock R. M., Flores J. Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhea virus rotavirus: comparison with homologous genes from four strains of human and animal rotaviruses. Virology. 1985 Mar;141(2):292–298. doi: 10.1016/0042-6822(85)90260-0. [DOI] [PubMed] [Google Scholar]
  8. Hardy M. E., Woode G. N., Xu Z. C., Gorziglia M. Comparative amino acid sequence analysis of VP4 for VP7 serotype 6 bovine rotavirus strains NCDV, B641, and UK. J Virol. 1991 Oct;65(10):5535–5538. doi: 10.1128/jvi.65.10.5535-5538.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hoshino Y., Sereno M. M., Midthun K., Flores J., Chanock R. M., Kapikian A. Z. Analysis by plaque reduction neutralization assay of intertypic rotaviruses suggests that gene reassortment occurs in vivo. J Clin Microbiol. 1987 Feb;25(2):290–294. doi: 10.1128/jcm.25.2.290-294.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Hoshino Y., Sereno M. M., Midthun K., Flores J., Kapikian A. Z., Chanock R. M. Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8701–8704. doi: 10.1073/pnas.82.24.8701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hoshino Y., Wyatt R. G., Greenberg H. B., Flores J., Kapikian A. Z. Serotypic similarity and diversity of rotaviruses of mammalian and avian origin as studied by plaque-reduction neutralization. J Infect Dis. 1984 May;149(5):694–702. doi: 10.1093/infdis/149.5.694. [DOI] [PubMed] [Google Scholar]
  12. Mackow E. R., Shaw R. D., Matsui S. M., Vo P. T., Benfield D. A., Greenberg H. B. Characterization of homotypic and heterotypic VP7 neutralization sites of rhesus rotavirus. Virology. 1988 Aug;165(2):511–517. doi: 10.1016/0042-6822(88)90595-8. [DOI] [PubMed] [Google Scholar]
  13. Matsuda Y., Nakagomi O., Offit P. A. Presence of three P types (VP4 serotypes) and two G types (VP7 serotypes) among bovine rotavirus strains. Arch Virol. 1990;115(3-4):199–207. doi: 10.1007/BF01310530. [DOI] [PubMed] [Google Scholar]
  14. Matsuno S., Hasegawa A., Mukoyama A., Inouye S. A candidate for a new serotype of human rotavirus. J Virol. 1985 May;54(2):623–624. doi: 10.1128/jvi.54.2.623-624.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Mebus C. A., Kono M., Underdahl N. R., Twiehaus M. J. Cell culture propagation of neonatal calf diarrhea (scours) virus. Can Vet J. 1971 Mar;12(3):69–72. [PMC free article] [PubMed] [Google Scholar]
  16. Murakami T., Hirano N., Chitose K., Tsuchiya K., Ono K., Sato F., Suzuki Y., Murakami Y. A survey on bovine rotavirus type 1-associated neonatal calf diarrhea in a beef herd. Nihon Juigaku Zasshi. 1987 Feb;49(1):23–30. doi: 10.1292/jvms1939.49.23. [DOI] [PubMed] [Google Scholar]
  17. Murakami Y., Nishioka N., Hashiguchi Y., Kuniyasu C. Serotypes of bovine rotaviruses distinguished by serum neutralization. Infect Immun. 1983 Jun;40(3):851–855. doi: 10.1128/iai.40.3.851-855.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nakagomi O., Nakagomi T., Akatani K., Ikegami N. Identification of rotavirus genogroups by RNA-RNA hybridization. Mol Cell Probes. 1989 Sep;3(3):251–261. doi: 10.1016/0890-8508(89)90006-6. [DOI] [PubMed] [Google Scholar]
  19. Offit P. A., Blavat G. Identification of the two rotavirus genes determining neutralization specificities. J Virol. 1986 Jan;57(1):376–378. doi: 10.1128/jvi.57.1.376-378.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Qian Y., Green K. Y. Human rotavirus strain 69M has a unique VP4 as determined by amino acid sequence analysis. Virology. 1991 May;182(1):407–412. doi: 10.1016/0042-6822(91)90691-4. [DOI] [PubMed] [Google Scholar]
  21. Ruiz A. M., López I. V., López S., Espejo R. T., Arias C. F. Molecular and antigenic characterization of porcine rotavirus YM, a possible new rotavirus serotype. J Virol. 1988 Nov;62(11):4331–4336. doi: 10.1128/jvi.62.11.4331-4336.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Snodgrass D. R., Fitzgerald T., Campbell I., Scott F. M., Browning G. F., Miller D. L., Herring A. J., Greenberg H. B. Rotavirus serotypes 6 and 10 predominate in cattle. J Clin Microbiol. 1990 Mar;28(3):504–507. doi: 10.1128/jcm.28.3.504-507.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Taniguchi K., Hoshino Y., Nishikawa K., Green K. Y., Maloy W. L., Morita Y., Urasawa S., Kapikian A. Z., Chanock R. M., Gorziglia M. Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies. J Virol. 1988 Jun;62(6):1870–1874. doi: 10.1128/jvi.62.6.1870-1874.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Taniguchi K., Pongsuwanna Y., Choonthanom M., Urasawa S. Nucleotide sequence of the VP7 gene of a bovine rotavirus (strain 61A) with different serotype specificity from serotype 6. Nucleic Acids Res. 1990 Aug 11;18(15):4613–4613. doi: 10.1093/nar/18.15.4613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Taniguchi K., Urasawa T., Pongsuwanna Y., Choonthanom M., Jayavasu C., Urasawa S. Molecular and antigenic analyses of serotypes 8 and 10 of bovine rotaviruses in Thailand. J Gen Virol. 1991 Dec;72(Pt 12):2929–2937. doi: 10.1099/0022-1317-72-12-2929. [DOI] [PubMed] [Google Scholar]
  27. Urasawa S., Urasawa T., Wakasugi F., Kobayashi N., Taniguchi K., Lintag I. C., Saniel M. C., Goto H. Presumptive seventh serotype of human rotavirus. Arch Virol. 1990;113(3-4):279–282. doi: 10.1007/BF01316680. [DOI] [PubMed] [Google Scholar]
  28. Wyatt R. G., Greenberg H. B., James W. D., Pittman A. L., Kalica A. R., Flores J., Chanock R. M., Kapikian A. Z. Definition of human rotavirus serotypes by plaque reduction assay. Infect Immun. 1982 Jul;37(1):110–115. doi: 10.1128/iai.37.1.110-115.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Xu L., Harbour D., McCrae M. A. Sequence of the gene encoding the major neutralization antigen (VP7) of serotype 10 rotavirus. J Gen Virol. 1991 Jan;72(Pt 1):177–180. doi: 10.1099/0022-1317-72-1-177. [DOI] [PubMed] [Google Scholar]
  30. Zheng S. L., Woode G. N., Melendy D. R., Ramig R. F. Comparative studies of the antigenic polypeptide species VP4, VP6, and VP7 of three strains of bovine rotavirus. J Clin Microbiol. 1989 Sep;27(9):1939–1945. doi: 10.1128/jcm.27.9.1939-1945.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES