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. 1993 Mar;31(3):528–532. doi: 10.1128/jcm.31.3.528-532.1993

Polymerase chain reaction-amplified nonradioactive probes for identification of Fusobacterium nucleatum.

A I Bolstad 1, H B Jensen 1
PMCID: PMC262814  PMID: 8458947

Abstract

A polymerase chain reaction probe with 100% sequence identity to 120 deoxyribonucleotides of Fusobacterium nucleatum Fev1, coding for a part of the 40-kDa major outer membrane protein, was labeled with the steroid hapten digoxigenin. The probe was compared with various degenerate oligonucleotide probes and found to tolerate much more stringent washing conditions. It was therefore superior in distinguishing, by means of Southern blots and slot blots, F. nucleatum from other oral gram-negative bacteria in the periodontal pocket and from other fusobacterial species and in distinguishing among different strains of F. nucleatum. F. periodonticum was found to be more similar to F. nucleatum than the other fusobacterial species tested.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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