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. 1993 Mar;31(3):533–539. doi: 10.1128/jcm.31.3.533-539.1993

Molecular typing of Pseudomonas pseudomallei: restriction fragment length polymorphisms of rRNA genes.

A E Lew 1, P M Desmarchelier 1
PMCID: PMC262815  PMID: 7681436

Abstract

The aim of this study was to develop a typing scheme for Pseudomonas pseudomallei by comparison of patterns of restriction fragment length polymorphisms in rRNA genes (ribotyping). BamHI restriction digests of 100 isolates from various animal (34), human (58), and environmental (6) sources, including six reference strains, were hybridized to Escherichia coli 16S and 23S rRNAs. A chemiluminescent labelling and detection system was used to visualize bands. On the basis of patterns, the strains were classified into 22 different groups, with the largest containing 29 isolates. While most of the ribotypes were not exclusive to a particular source, some ribotypes were restricted to a particular geographic area or to either a human or a particular animal species. Application of the typing scheme to isolates of four independent outbreaks among animals showed that certain ribotypes predominated. The study demonstrated ribotyping to be a useful tool in epidemiological investigations of melioidosis.

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Selected References

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  1. Altwegg M., Hickman-Brenner F. W., Farmer J. J., 3rd Ribosomal RNA gene restriction patterns provide increased sensitivity for typing Salmonella typhi strains. J Infect Dis. 1989 Jul;160(1):145–149. doi: 10.1093/infdis/160.1.145. [DOI] [PubMed] [Google Scholar]
  2. Ashdown L. R. An improved screening technique for isolation of Pseudomonas pseudomallei from clinical specimens. Pathology. 1979 Apr;11(2):293–297. doi: 10.3109/00313027909061954. [DOI] [PubMed] [Google Scholar]
  3. BRINDLE C. S., COWAN S. T. Flagellation and toxonomy of Whitmore's bacillus. J Pathol Bacteriol. 1951 Oct;63(4):571–575. doi: 10.1002/path.1700630403. [DOI] [PubMed] [Google Scholar]
  4. Blumberg H. M., Kiehlbauch J. A., Wachsmuth I. K. Molecular epidemiology of Yersinia enterocolitica O:3 infections: use of chromosomal DNA restriction fragment length polymorphisms of rRNA genes. J Clin Microbiol. 1991 Nov;29(11):2368–2374. doi: 10.1128/jcm.29.11.2368-2374.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Blumberg H. M., Rimland D., Kiehlbauch J. A., Terry P. M., Wachsmuth I. K. Epidemiologic typing of Staphylococcus aureus by DNA restriction fragment length polymorphisms of rRNA genes: elucidation of the clonal nature of a group of bacteriophage-nontypeable, ciprofloxacin-resistant, methicillin-susceptible S. aureus isolates. J Clin Microbiol. 1992 Feb;30(2):362–369. doi: 10.1128/jcm.30.2.362-369.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Bruce K. D., Jordens J. Z. Characterization of noncapsulate Haemophilus influenzae by whole-cell polypeptide profiles, restriction endonuclease analysis, and rRNA gene restriction patterns. J Clin Microbiol. 1991 Feb;29(2):291–296. doi: 10.1128/jcm.29.2.291-296.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. CHAMBON L., FOURNIER J. Constitution antigénique de Malleomyces pseudo-Mallei. I. Caractères morphologiques, culturaux, biochimiques et variations de type immunologique. Ann Inst Pasteur (Paris) 1956 Sep;91(3):355–362. [PubMed] [Google Scholar]
  8. COTTEW G. S. Melioidosis in sheep in Queens land; a description of the causal organism. Aust J Exp Biol Med Sci. 1950 Nov;28(6):677–683. [PubMed] [Google Scholar]
  9. Dance D. A. Pseudomonas pseudomallei: danger in the paddy fields. Trans R Soc Trop Med Hyg. 1991 Jan-Feb;85(1):1–3. doi: 10.1016/0035-9203(91)90134-k. [DOI] [PubMed] [Google Scholar]
  10. Dodin A., Fournier J. Antigènes précipitants et antigènes agglutinants de Pseudomonas pseudomallei (B. de Whitmore). I. Complex thermostable et complex thermolabile. Typage sérologique. Ann Inst Pasteur (Paris) 1970 Aug;119(2):211–221. [PubMed] [Google Scholar]
  11. Grimont F., Grimont P. A. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol. 1986 Sep-Oct;137B(2):165–175. doi: 10.1016/s0769-2609(86)80105-3. [DOI] [PubMed] [Google Scholar]
  12. Guard R. W. Melioidosis in Australia. A review. Aust Fam Physician. 1987 Feb;16(2):119–123. [PubMed] [Google Scholar]
  13. Hinojosa-Ahumada M., Swaminathan B., Hunter S. B., Cameron D. N., Kiehlbauch J. A., Wachsmuth I. K., Strockbine N. A. Restriction fragment length polymorphisms in rRNA operons for subtyping Shigella sonnei. J Clin Microbiol. 1991 Nov;29(11):2380–2384. doi: 10.1128/jcm.29.11.2380-2384.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Irino K., Grimont F., Casin I., Grimont P. A. rRNA gene restriction patterns of Haemophilus influenzae biogroup aegyptius strains associated with Brazilian purpuric fever. J Clin Microbiol. 1988 Aug;26(8):1535–1538. doi: 10.1128/jcm.26.8.1535-1538.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Kiehlbauch J. A., Plikaytis B. D., Swaminathan B., Cameron D. N., Wachsmuth I. K. Restriction fragment length polymorphisms in the ribosomal genes for species identification and subtyping of aerotolerant Campylobacter species. J Clin Microbiol. 1991 Aug;29(8):1670–1676. doi: 10.1128/jcm.29.8.1670-1676.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Koblavi S., Grimont F., Grimont P. A. Clonal diversity of Vibrio cholerae O1 evidenced by rRNA gene restriction patterns. Res Microbiol. 1990 Jul-Aug;141(6):645–657. doi: 10.1016/0923-2508(90)90059-y. [DOI] [PubMed] [Google Scholar]
  17. Leelarasamee A., Bovornkitti S. Melioidosis: review and update. Rev Infect Dis. 1989 May-Jun;11(3):413–425. doi: 10.1093/clinids/11.3.413. [DOI] [PubMed] [Google Scholar]
  18. Lertmemongkolchai G., Manmontri W., Leelayuwat C., Romphruk A., Waropastrakul S. Immunoblot analysis to demonstrate antigenic variability of clinical isolated. Pseudomonas pseudomallei. Asian Pac J Allergy Immunol. 1991 Jun;9(1):5–8. [PubMed] [Google Scholar]
  19. O'Farrell P. H., Kutter E., Nakanishi M. A restriction map of the bacteriophage T4 genome. Mol Gen Genet. 1980;179(2):421–435. doi: 10.1007/BF00425473. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Owen R. J. Chromosomal DNA fingerprinting--a new method of species and strain identification applicable to microbial pathogens. J Med Microbiol. 1989 Oct;30(2):89–99. doi: 10.1099/00222615-30-2-89. [DOI] [PubMed] [Google Scholar]
  21. Prevost G., Jaulhac B., Piemont Y. DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates. J Clin Microbiol. 1992 Apr;30(4):967–973. doi: 10.1128/jcm.30.4.967-973.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. RIMINGTON R. A. Melioidosis in north Queensland. Med J Aust. 1962 Jan 13;49(1):50–53. doi: 10.5694/j.1326-5377.1962.tb76106.x. [DOI] [PubMed] [Google Scholar]
  23. Salvi R. J., Ahroon W., Saunders S. S., Arnold S. A. Evoked potentials: computer-automated threshold-tracking procedure using an objective detection criterion. Ear Hear. 1987 Jun;8(3):151–156. [PubMed] [Google Scholar]
  24. Schaffer H. E., Sederoff R. R. Improved estimation of DNA fragment lengths from Agarose gels. Anal Biochem. 1981 Jul 15;115(1):113–122. doi: 10.1016/0003-2697(81)90533-9. [DOI] [PubMed] [Google Scholar]
  25. Stull T. L., LiPuma J. J., Edlind T. D. A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis. 1988 Feb;157(2):280–286. doi: 10.1093/infdis/157.2.280. [DOI] [PubMed] [Google Scholar]
  26. Tee W., Lambert J., Smallwood R., Schembri M., Ross B. C., Dwyer B. Ribotyping of Helicobacter pylori from clinical specimens. J Clin Microbiol. 1992 Jun;30(6):1562–1567. doi: 10.1128/jcm.30.6.1562-1567.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Thomas A. D., Forbes-Faulkner J., Parker M. Isolation of Pseudomonas pseudomallei from clay layers at defined depths. Am J Epidemiol. 1979 Oct;110(4):515–521. doi: 10.1093/oxfordjournals.aje.a112832. [DOI] [PubMed] [Google Scholar]
  28. Woods T. C., Helsel L. O., Swaminathan B., Bibb W. F., Pinner R. W., Gellin B. G., Collin S. F., Waterman S. H., Reeves M. W., Brenner D. J. Characterization of Neisseria meningitidis serogroup C by multilocus enzyme electrophoresis and ribosomal DNA restriction profiles (ribotyping). J Clin Microbiol. 1992 Jan;30(1):132–137. doi: 10.1128/jcm.30.1.132-137.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yogev D., Halachmi D., Kenny G. E., Razin S. Distinction of species and strains of mycoplasmas (mollicutes) by genomic DNA fingerprints with an rRNA gene probe. J Clin Microbiol. 1988 Jun;26(6):1198–1201. doi: 10.1128/jcm.26.6.1198-1201.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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