Abstract
The liver is a key organ for numerous metabolic pathways and involves many inherited diseases that, although being different in their pathology, are often caused by lack or overproduction of a critical gene product in the diseased cells. In principle, a straightforward method to fix such problem is to introduce into these cells with a gene-coding sequence to provide the missing gene product or with the nucleic acid sequence to inhibit production of the excessive gene product. Practically, however, success of nucleic acid-based pharmaceutics is dependent on the availability of a method capable of delivering nucleic acid sequence in the form of DNA or RNA to liver cells. In this review, we will summarize the progress toward the development of physical methods for nucleic acid delivery to the liver. Emphasis is placed on the mechanism of action, pros, and cons of each method developed so far. We hope the information provided will encourage new endeavor to improve the current methodologies or develop new strategies that will lead to safe and effective delivery of nucleic acids to the liver.
Key words: gene delivery, liver, nonviral vectors, physical method, transfection
INTRODUCTION
The liver is the largest organ in the body with functions crucial to sustaining life and is responsible for many genetic and metabolic disorders. Significant efforts have been made in the past toward elucidation of mechanisms underlying different liver diseases. Progress made in both basic and clinical research in recent years has led to the development of gene therapy as an alternative to orthotropic liver transplantation, which is the only effective therapy currently available for many liver diseases. The challenge for liver gene therapy, however, is the development of a method to allow safe and effective delivery of therapeutic gene to liver cells. The following sections summarize the fundamental aspects of hepatic gene delivery with the emphasis on physical approaches.
STRUCTURE AND PHYSIOLOGY OF THE LIVER AND BARRIERS OF NUCLEIC ACID DELIVERY
The liver is situated just below the diaphragm and the upper right side of the stomach. In human, it comprises about 2% of the total adult body weight and consists of four lobes (right, left, quadrate, and caudate lobe). At the microscopic and functional level, the liver is composed of lobules each of which ranges from 1 to 2.5 mm in diameter and contains a mass of cells (2 × 105 cells per milligram) (1). The outline of the lobules in human liver are irregular, but in some of the lower animal species (for example, the pig), they are well-defined and have hexagonal shape (2). The base of the lobule is clustered around the smallest hepatic vein (central vein). The remaining part of each lobule is imperfectly isolated from the surrounding lobules by a thin stratum of connective tissue in which a plexus of blood vessels and ducts is contained (1,3). In some animals, as in the pig, the lobules are completely isolated from one another by the interlobular connective tissue (2).
The liver consists of plural types of cells. The hepatocytes are in polyhedral shape. They vary in size from 12 to 25 μm in diameter and contain one or sometimes two distinct nuclei in each cell. The hepatocytes face, the perisinusoidal space called space of Disse. The neighboring hepatocytes are connected by tight junctions, gap junctions, and desmosomes. The sinusoids are made of endothelial cells, phagocytic Kupffer cells, stellate cells (Ito cells), and pit cells. The Kupffer cell is the macrophage attached to the sinusoidal endothelium and responsible for the removal of invading particles into the blood. Ito cells lie in the space of Disse and have a function of storage of retinoids, and with hepatic injury, they transform to myofibroblast-like cells and produce fibrous tissue. Pit cells are one of the natural killer cells types that are attached to the sinusoidal surface of the endothelium (1).
The liver has an unusual blood supply system. Approximately 1,300 mL of blood flow into the liver every minute, representing about 25% of total cardiac output. About 80% of the liver blood is transported via the portal vein carrying nutrients or digested food from the digestive tract. The other 20% come via hepatic artery carrying oxygen-enriched blood from the heart. The hepatic artery and the portal vein branch into a network of small blood vessels that empty into the sinusoids where the venous and arterial blood mix (1). The endothelial wall of the sinusoids is discontinuous (or fenestrated) with pores of about 100 nm in diameter, which brings blood substances or particles below 100 nm into direct contact with the liver cells beyond the endothelium. The sinusoids drain into the central veins which join to form the hepatic vein, from which blood leaves the liver, enters the inferior vena cava, and returns to the heart (1).
The bile ducts begin at little passages in the liver cells that communicate with bile capillaries. These passages are merely little channels or spaces left between the contiguous surfaces of two or more hepatocytes. These channel-like bile ducts are always separated from the blood capillaries by at least half the width of a liver cell and open into the interlobular bile ducts which run in Glisson’s capsule accompanying with the portal vein and hepatic artery. The walls of the bile ducts consist of a connective tissue coat in which there are muscle cells arranged both circularly and longitudinally and an epithelial layer consisting of short columnar cells resting on a distinct basement membrane. The exterior coats of the large bile ducts is composed of strong fibrotic tissue with a certain amount of muscular tissue arranged for the most part in a circular manner around the duct. The interior mucous coat of the bile ducts is continuous with the lining membrane of the bile ducts and gallbladder and also with that of the duodenum. The bile juice enters the duodenum through papilla (1).
The functions of the liver are numerous, working closely with nearly every system and process in the human body. The hepatic parenchymal cells have a broad range of synthetic and catabolic functions. The liver is the primary organ responsible for the metabolism of carbohydrates, lipids, proteins, and heme and for removal of toxins, hormones, and aged red blood cells. The liver is responsible for synthesizing most plasma proteins (with the exception of immunoglobulins), bile acids, cholesterol, and heparin and serves as the principal site for storage of iron, glycogen, lipids, and vitamins. The liver also plays an important role in the detoxification of many drugs and excretion of metabolic end products such as bilirubin, ammonia, and urea.
Because of its sophisticated and important function in regulating metabolism and maintaining homeostasis, the liver is a key organ for most metabolic pathways, and therefore, numerous inherited diseases have their origin in this organ. Candidate diseases include genetic disorders such as hemochromatosis, hemophilia A and B, alpha 1 antitrypsin deficiency, Wilson’s disease, Crigler–Najjar syndrome type I, ornithine transcarbamylase deficiency, type IIa familial hypercholesterolemia, and afibrinogenemia. Therefore, the medical significance and afferent and efferent pathways to the liver have made the liver an ideal target for gene therapy studies.
The primary barrier for nucleic acid delivery to liver cells is the plasma membrane. If DNA molecules are larger than 100 nm, the endothelium also serves as the barrier for intrahepatocyte delivery. As far as gene delivery to the liver is concerned, the true challenge is to deliver nucleic acids to most hepatocytes in the liver, if not all, without causing tissue damage.
CONSIDERATIONS ON NUCLEIC ACID DELIVERY FOR TREATMENT OF LIVER DISEASES
The most crucial aspect of nucleic acid-based therapy for liver-associated diseases is the availability of a suitable delivery system. The subject of gene delivery and gene therapy has been well studied in the past 30 years primarily with viral vectors (4,5). Analogous to liver transplantation, early work on liver gene therapy employed ex vivo strategy involving re-implantation of the patient’s hepatocytes after viral gene transfer. In fact, this strategy was used for liver-directed gene therapy against hypercholesterolemia (6). Due to the invasiveness and high cost of the ex vivo approach, much efforts have been made to find an alternative and easier in vivo method. To target the liver specifically, researchers have opted to perform injections of viral vectors directly into the afferent vessels of the liver (portal vein) or the bile duct instead of the peripheral circulation. However, viral vectors employed in these studies entail some disadvantages. For example, retroviral vector-mediated delivery necessitates partial hepatectomy to trigger hepatocyte division (7). Adenoviral vectors induce immune response that causes the destruction of transduced hepatocytes (8) and prevents repeated administration. Adeno-associated viral vector with small loading capacity suffers from the same problem of immune response. While immune suppression of the host improved transgene expression and decreased liver destruction (9), the necessity of continued suppression to maintain the level of gene expression renders this approach less ideal for human gene therapies.
Another approach for liver-specific gene delivery is the use of synthetic compounds called synthetic vectors. Much work for gene delivery to the liver using synthetic vectors has focused on targeted gene delivery through asialoglycoprotein receptor (ASGP-R) (10) and subsequently the transferrin receptor (11). ASGP-R is a membrane protein localized to the sinusoidal surface of hepatocytes. It is a prototypic member of the C-type lectin family in mammals. Its ligand-binding affinity is dictated by the number (3 > 2 > 1), type (GalNAc > Gal), and conformation of terminal residues on N-glycans. A natural triantennary N-glycan possesses relatively high affinity (Kd = 4 nM) due to a precise geometric fit between three terminal galactose residues and complementary binding pockets on the ASGP-R (10,12). The receptor can recognize a variety of other galactosylated polymers, many of which have been used in gene delivery. The ASGP-R internalizes its ligand via coated pits, de-associates from ligand in a prelysosomal compartment, and then recycles to the cell surface (10). Early studies by Wu and colleagues (13) and a more recent study by our laboratory (14) demonstrated in vivo gene delivery by targeting the ASGP-R following intravenous injection of plasmid DNA/carrier complex. The work by Kwoh and coworkers (15), Hashida and coworkers (16), and Rice and coworkers (17) have also demonstrated the utility of ASGP-R targeted gene delivery as a model system for studying the relationship between carrier structures and transient gene expression. Studies from Kwoh et al. and Rice’s group indicate that specific targeting of electropositive DNA complexes to hepatocytes requires the use of targeting ligand and a large volume of solution for injection (15,17). Unfortunately, the gene expression achieved in each of these studies has been low and short in duration.
Concerns on virus-induced toxicity and low delivery efficiency of synthetic vectors including those of cationic lipids (18), cationic polymers (19), and naturally occurring compounds (20) have inspired significant effort in recent years to find new method for nucleic acid delivery in its original or “naked” form. An obvious advantage of naked DNA is its simplicity and safety. As part of composition in biological system, nucleic acids as a chemical identity are not toxic, readily biodegradable, and impermeable across the cell membrane. The following sections will summarize the progress in the area of naked DNA delivery. Although discussion was centered on gene delivery, the techniques and the underlying principles are applicable to other forms of nucleic acids such as DNA, RNA, and nucleic acid-based derivatives. The overall emphasis of our discussion is on the rationale, pros, and cons of each method for liver-based nucleic acid delivery.
PROGRESS IN DEVELOPMENT OF PHYSICAL METHODS FOR NUCLEIC ACID DELIVERY TO LIVER
At the molecular level, the objective of delivery of nucleic acid sequence to the liver is to accomplish one of the following: (a) replacement of a missing gene product; (b) overproduction of a gene product; (c) DNA vaccine; (d) generation of hormonal proteins to regulate cell growth (e.g., growth hormone), cell differentiation (e.g., cytokines), or metabolism (e.g., insulin); (e) inhibition of gene expression (delivery of oligonucleotides, siRNA, shRNA); or (f) DNA repair (single-strand DNA or DNA/RNA hybrid). Because nucleic acids are nonpermeable across cell membrane due to their large size and hydrophilicity, the physical method of intrahepatic nucleic acid delivery aims at overcoming the plasma membrane barrier of liver cells. The routes of delivery could be direct injection into the liver, intraportal or intrahepatic vein, intrahepatic artery, intrabile duct, or systemic. The physical forces employed include pressure, shock wave, electric pulse, and ultrasound wave. Table I summarizes various aspects of currently available physical methods for nucleic acid delivery to the liver.
Table I.
Method | Force | Application | Advantages | Limitation/problem |
---|---|---|---|---|
Needle injection | Mechanic | Intratissue | Simple | Low efficiency, limited to needle track |
Gene gun | Momentum | Topical | Good efficiency | Limited to small area, need surgical procedure for internal organs |
Electroporation | Electric pulse | Topical, intratissue | High efficiency | Limited to small area, need surgical procedure, tissue damage |
Sonoporation | Shock wave | Intratissue | Region specific | Low efficiency, tissue damage |
Hydrodynamic delivery | Hydrodynamic pressure | Intravascular | Simplicity, high efficiency, region-specific | Need catheterization in large animals |
Intrahepatic Gene Delivery by Needle Injection
Inspired by the success of gene delivery to muscle cells through direct injection of plasmid DNA into muscle in mice (21), Hickman et al. injected reporter plasmids into mouse liver and demonstrated gene expression in liver cells, primarily near the needle track (22). A similar procedure was also performed as the treatment of hepatocellular carcinoma involving intratumor injection of plasmid DNA containing p53 gene (23). Although three of the five patients who received percutaneous injection of wild-type p53 showed objective tumor response with reduction of the tumor size and decreasing of serum alpha-fetoprotein, gene delivery was limited to cells on and near the needle track and the overall delivery efficiency was extremely low. The mechanism of intracellular gene delivery by needle injection appears to involve the penetration of sharp needle across the cells on its path, breaking cell membrane and allowing plasmid DNA to enter the cells before the broken cell membrane reseals. Consequently, tissue damage is always produced with this procedure and the total number of cells with successful nucleic acid delivery is limited.
Intrahepatic Gene Delivery by Gene Gun (Ballistic Bombardment)
Gene gun-based gene delivery was first employed in 1987 for gene delivery to plant cells (24), and since the 1990s, has been used in cultured cells and in vivo for transfection (25). It involves propelling the DNA-coated gold particles against cells. Intracellular gene transfer is achieved by adjusting the propelling pressure, particle size, and distance between the gene gun and target cells. Yang et al. (26) and Kuriyama et al. (27) have shown in mouse that gene transfer to liver cells can be achieved using the gene gun approach. However, the procedure requires a surgical procedure to expose the liver and gene expression was found only in cells near the exterior surface of the targeted area. Because of the shallow penetration of DNA, particle bombardment through a gene gun appears more appropriate for gene delivery to skin for vaccination and immune therapy (28–32). Roberts et al. have demonstrated the safe and effective particle-mediated epidermal delivery of DNA vaccine against hepatitis B virus (HBV) (30). Chang et al. have reported enhancement in efficiency by repeated bombardment (33).
Intrahepatic Gene Delivery by Electroporation
Electroporation was first utilized for gene transfer to mammalian cells by Neumann et al. in 1982 (34) and has been extensively studied in recent years as an effective method for gene delivery not only in vitro but also in vivo (35). As evidenced by its name, gene transfer by this technique is achieved by generating membrane pores on cells through electric pulse, normally requiring a high voltage applied to a relatively small area of tissue. The delivery efficiency is determined by the pulse intensity, duration and frequency, and the type of cells as well (36). Using this method, Heller et al. (37) and Suzuki et al. (38) reported the successful naked DNA transfer to the liver in rats in the late 1990s. Liu et al. reported efficient gene transfer to mouse liver by electroporation following tail vein administration of the naked DNA (39). Sakai et al. showed that electric pulses applied to the mouse left lateral lobe after intravenous injection of naked plasmid DNA results in regional gene expression centered on the area where the pulses were applied (40). Transfected cells were more broadly distributed with the systemic injection compared to a local injection. These reports suggest that systemic injection is preferable to the regional injection of DNA to the liver when electroporation was used. By employing this procedure, Jaichandran et al. demonstrated the phenotypic correction in hemophilic mice when plasmid containing cDNA of factor VIII was transferred into the mouse liver (41). The major drawback of the electroporation-mediated gene transfer to liver cells in vivo is the involvement of surgical procedure to expose and allow insertion of the electrodes into the liver or placement of the plate electrodes onto the liver surface. In addition, the area impacted by each procedure is rather limited. While effective in increasing the number of transfected cells, high voltage often results in significant tissue damage. It appears that a new device with optimal electrical parameters and specifically designed electrodes is needed before electroporation can be clinically useful for liver gene transfer.
Ultrasound-Mediated Gene Transfer
This technique was developed in the 1990s to facilitate gene transfer to mammalian cells in vitro (42–44) and later in tissue (45–63). A more popular name reflecting the mechanism of action for this technique is sonoporation, indicating the creation of acoustic membrane pores on the cells through which nucleic acids diffuse into cells. Gene transfer efficiency appears to be controlled by pulse intensity, frequency, and duration (64). Sonoporation in vitro often results in high cell mortality with a small fraction of surviving cells showing gene transfer. The potential for in vivo use has been a subject of intensive research. Sonoporation-enhanced gene transfer has been explored in the cornea (46), brain (47), central nervous system (48,49), spinal cord (50), bone (51), peritoneal cavity (52), kidney (53), pancreas (54), liver (55,56), embryonic tissue (57), dental pulp (58), solid tumor (59), muscle (60,61), and heart (62,63). More recent studies have shown in mouse liver that phase contrast medium consisting of gas-filled microbubbles enhances gene delivery efficiency by increasing the cavitation of cell membrane (55,56). Since ultrasound is clinically used as a noninvasive diagnostic imaging tool and as a low-invasive method for shock wave treatment for urolithiasis, cholecystolithiasis, and choledocholithiasis, the technology would be extremely useful for nucleic acid delivery to the liver if the problem of low delivery efficiency and tissue damage associated with the procedure can be solved.
Hydrodynamic Delivery
In 1999, Liu et al. (65) and Zhang et al. (66) reported that a rapid injection of a large volume of DNA solution via the mouse tail vein delivered reporter gene into hepatocytes with high efficiency. This technique, more commonly called hydrodynamic delivery, is the simplest method for intrahepatic gene delivery. It involves an injection in 5–8 s of 8–10% of body weight in volume of isotonic DNA solution into the tail vein of a mouse. The mechanism of action underlying this procedure includes: (a) induction of cardiac congestion and elevation of pressure in the inferior vena cava; (b) retrograde flow of DNA solution into the liver; (c) enlargement of the fenestrae and generation of transient membrane defect on plasma membrane of hepatocytes; and (d) gene transfer into hepatocytes (67). Suda et al. have shown that the hydrodynamic impact on the liver is transient and reversible. It takes about 24–36 h for the liver endothelium to recover functionally and less than 1 min for the plasma membrane of hepatocytes to reseal (68). Using this method, 30–40% of the hepatocytes in the liver are transfected by a single tail vein injection of less than 50 μg of plasmid DNA into a mouse (65). Because of its simplicity, high efficiency, and reproducibility, hydrodynamic delivery has become a routine method for the delivery of DNA, siRNA, proteins, small compounds, and even viral vectors into the hepatocytes in vivo (69–73). Since its development in 1999, this procedure has been widely used for gene expression, gene knockdown, functional analysis of genetic elements, and establishing disease models in research animals (69,73).
While effective in rodents, hydrodynamics-based procedure has not been considered favorably for gene delivery in large animals or humans because injection of approximately 10% of body weight in volume (∼7 L for a 70-kg man) is considered impractical and unsafe. However, the volume required can be reduced if a localized injection directly into the liver vasculature can be performed. For example, Eastman et al. have explored the possibility of hydrodynamic gene delivery through a catheter inserted into the hepatic vein under fluoroscopic guidance (74). They demonstrated in rabbits that a volume of 15 mL/kg can be safely injected to an isolated rabbit liver. Similarly, Kabayashi’s group reported delivery of green fluorescent protein-containing plasmid DNA to the left lateral lobe of pig liver by catheterization and occlusion of the portal vein (75). With slight modification in procedure, Alino et al. (76), Fabre et al. (77), and Brunetti-Pierri (78) also demonstrated the feasibility for localized hydrodynamic delivery to the liver.
Realizing the intravascular pressure as the key parameter for hydrodynamic gene delivery to the liver, Suda et al. have recently developed a computer-controlled injection device aimed at the application of hydrodynamic delivery to humans (79). With a real-time feedback system based on vascular pressure to control the injection, the volume necessary for maximum effect of intrahepatic delivery was reduced from the original 10% of body weight to the current 5% for the entire liver of a mouse (79). By combining the computer-controlled device with image-guided catheterization technique, Kamimura et al. (submitted for publication) have recently established a procedure in swine for regional gene delivery to the liver. Assessments on cardiac function, tissue damage, and homeostasis revealed no abnormality on the animals tested (Kamimura et al., submitted for publication). This image-guided, lobe-specific hydrodynamic procedure has great potential to become the method of gene delivery to the liver for human gene therapy.
FUTURE PERSPECTIVES
Nucleic acid-based therapeutics has been developed for the treatment of inherited and acquired diseases. The challenge, however, has been to develop a safe and effective method to bring therapeutic molecules into cells where treatment is needed. Biological, chemical, and physical principles are being utilized for the development of viral, synthetic, and physical methods, respectively. Significant progress has been made since the first gene therapy trial in 1990 (80) with respect to each type of vectors or a particular method. As far as the physical methods are concerned, the mechanisms of gene delivery by needle injection, ballistic bombardment (gene gun), electroporation, sonoporation, and hydrodynamics-based procedure have been fairly well-understood and all of these techniques are commonly used as a tool in research laboratories. With respect to their potential for liver-based clinic applications, however, hydrodynamic gene delivery appears to be most effective. Since its development in the late 1990s, this technique has become the routine method for liver transfection and been employed for the delivery of genes for gene therapy studies, siRNA for target validation, and viral genome for establishing HBV infection in mouse (69,73). The recent development of the computer-controlled injection device has made it possible for applying the hydrodynamic principle to gene delivery in large animals and possibly humans (79). Employing the image-guided catheterization technology to place the catheter to a specific site in a hepatic vein, one can now follow a standard procedure and perform site-specific hydrodynamic delivery not only to the liver but also to other organs (Kamimura et al., submitted for publication). To develop a clinically viable procedure for the liver, we will need participations of hepatologists who are trained to deal with liver diseases for establishing the optimal hydrodynamic parameters (injection speed, injection volume, pressure profiles) and for further improvements. With continuing efforts and the significant progress made in the past, it is foreseeable in the near future that the challenge for the development of a safe, effective, and clinically applicable method for nucleic acid delivery will be met. Consequently, the true value and benefit of nucleic acid-based therapy may be fully realized.
Acknowledgment
The authors’ research was supported in part by National Institutes of Health grants EB002946, EB007357, and HL075542.
References
- 1.Sheila S., James D. Diseases of the Liver and Biliary System. 11. Oxford: Blackwell Science; 2002. [Google Scholar]
- 2.Dyce K. M., Sack W. O., Wensing C. J. G. Textbook of Veterinary Anatomy. Philadelphia: Saunders; 1987. [Google Scholar]
- 3.Rappaport A. M. The microcirculatory acinar concept of normal and pathological hepatic structure. Beitr. Pathol. 1976;157:215–243. doi: 10.1016/s0005-8165(76)80083-2. [DOI] [PubMed] [Google Scholar]
- 4.Thomas C. E., Ehrhardt A., Kay M. A. Progress and problems with the use of viral vectors for gene therapy. Nat. Rev. Genet. 2003;4:346–358. doi: 10.1038/nrg1066. [DOI] [PubMed] [Google Scholar]
- 5.Kootstra N. A., Verma I. M. Gene therapy with viral vectors. Ann. Rev. Pharmacol. Toxicol. 2003;43:413–439. doi: 10.1146/annurev.pharmtox.43.100901.140257. [DOI] [PubMed] [Google Scholar]
- 6.Wilson J. M., Grossman M., Raper S. E., Baker J. R. J., Newton R. S., Thoene J. G. Ex vivo gene therapy of familial hypercholesterolemia. Hum. Gene Ther. 1992;3:179–222. doi: 10.1089/hum.1992.3.2-179. [DOI] [PubMed] [Google Scholar]
- 7.Branchereau S., Calise D., Ferry N. Factors influencing retroviral-mediated gene transfer into hepatocytes in vivo. Hum. Gene Ther. 1994;5:803–808. doi: 10.1089/hum.1994.5.7-803. [DOI] [PubMed] [Google Scholar]
- 8.Marshall E. Gene therapy death prompts review of adenovirus vector. Science. 1999;286:2244–2245. doi: 10.1126/science.286.5448.2244. [DOI] [PubMed] [Google Scholar]
- 9.Bessis N., GarciaCozar F. J., Boissier M. C. Immune responses to gene therapy vectors: influence on vector function and effector mechanisms. Gene Ther. 2004;11:S10–S17. doi: 10.1038/sj.gt.3302364. [DOI] [PubMed] [Google Scholar]
- 10.Lodish H. F. Recognition of complex oligosaccharides by the multi-subunit asialoglycoprotein receptor. Trends Biochem. Sci. 1991;16:374–377. doi: 10.1016/0968-0004(91)90154-N. [DOI] [PubMed] [Google Scholar]
- 11.Yu W., Pirollo K. F., Rait A., Yu B., Xiang L. M., Huang W. Q., Zhou Q., Ertem G., Chang E. H. A sterically stabilized immunolipoplex for systemic administration of a therapeutic gene. Gene Ther. 2004;11:1434–1440. doi: 10.1038/sj.gt.3302304. [DOI] [PubMed] [Google Scholar]
- 12.Rice K. G., Weisz O. A., Barthel T., Lee R. T., Lee Y. C. Defined geometry of binding between triantennary glycopeptide and the asialoglycoprotein receptor of rat heptocytes. J. Biol. Chem. 1990;265:18429–18434. [PubMed] [Google Scholar]
- 13.Wu G. Y., Wilson J. M., Shalaby F., Grossman M., Shafritz D. A., Wu C. H. Receptor-mediated gene delivery in vivo. Partial correction of genetic an albuminemia in Nagase rats. J. Biol. Chem. 1991;266:14338–14342. [PubMed] [Google Scholar]
- 14.Kim K. S., Lei Y., Stolz D. B., Liu D. Bifunctional compounds for targeted hepatic gene delivery. Gene Ther. 2007;14:704–708. doi: 10.1038/sj.gt.3302917. [DOI] [PubMed] [Google Scholar]
- 15.Kwoh D. Y., Coffin C. C., Lollo C. P., Jovenal J., Banaszczyk M. G., Mullen P., Phillips A., Amini A., Fabrycki J., Bartholomew R. M., Brostoff S. W., Carlo D. J. Stabilization of poly-L-lysine/DNA polyplexes for in vivo gene delivery to the liver. Biochim. Biophys. Acta. 1999;1444:171–190. doi: 10.1016/s0167-4781(98)00274-7. [DOI] [PubMed] [Google Scholar]
- 16.Nishikawa M., Yamauchi M., Morimoto K., Ishida E., Takakura Y., Hashida M. Hepatocyte-targeted in vivo gene expression by intravenous injection of plasmid DNA complexed with synthetic multi-functional gene delivery system. Gene Ther. 2000;7:548–555. doi: 10.1038/sj.gt.3301140. [DOI] [PubMed] [Google Scholar]
- 17.Wadhwa M. S., Knoell D. L., Young A. P., Rice K. G. Targeted gene delivery with a low molecular weight glycopeptide carrier. Bioconjug. Chem. 1995;6:283–291. doi: 10.1021/bc00033a008. [DOI] [PubMed] [Google Scholar]
- 18.Liu D., Ren T., Gao X. Cationic transfection lipids. Curr. Med. Chem. 2003;10:1307–1315. doi: 10.2174/0929867033457386. [DOI] [PubMed] [Google Scholar]
- 19.Schaffert D., Wagner E. Gene therapy progress and prospects: synthetic polymer-based systems. Gene Ther. 2008;15:1131–1138. doi: 10.1038/gt.2008.105. [DOI] [PubMed] [Google Scholar]
- 20.Dang J. M., Leong K. W. Natural polymers for gene delivery and tissue engineering. Adv. Drug Deliv. Rev. 2006;58:487–499. doi: 10.1016/j.addr.2006.03.001. [DOI] [PubMed] [Google Scholar]
- 21.Wolff J. A., Malone R. W., Williams P., Chong W., Acsadi G., Jani A., Felgner P. L. Direct gene transfer into mouse muscle in vivo. Science. 1990;247:1465–1468. doi: 10.1126/science.1690918. [DOI] [PubMed] [Google Scholar]
- 22.Hickman M. A., Malone R. W., Lehmann-Bruinsma K., Sih T. R., Knoell D., Szoka F. C., Walzem R., Carlson D. M., Powell J. S. Gene expression following direct injection of DNA into liver. Hum. Gene Ther. 1994;5:1477–1483. doi: 10.1089/hum.1994.5.12-1477. [DOI] [PubMed] [Google Scholar]
- 23.Habib N. A., Ding S. F., el-Masry R., Mitry R. R., Honda K., Michail N. E., Dalla Serra G., Izzi G., Greco L., Bassyouni M., el-Toukhy M., Abdel-Gaffar Y. Preliminary report: the short-term effects of direct p53 DNA injection in primary hepatocellular carcinomas. Cancer Detect. Prev. 1996;20:103–107. [PubMed] [Google Scholar]
- 24.Klein R. M., Wolf E. D., Wu R., Sanford J. C. High-velocity microprojectiles for delivering nucleic acids into living cells. Biotechnology. 1992;24:384–386. [PubMed] [Google Scholar]
- 25.O’Brien J., Lummis S. C. R. An improved method of preparing microcarriers for biolistic transfection. Brain Res. Brain. Res. Protoc. 2002;10:12–15. doi: 10.1016/s1385-299x(02)00175-7. [DOI] [PubMed] [Google Scholar]
- 26.Yang N. S., Burkholder J., Roberts B., Martinell B., McCabe D. In vivo and in vitro gene transfer to mammalian somatic cells by particle bombardment. Proc. Natl. Acad. Sci. U. S. A. 1990;87:9568–9572. doi: 10.1073/pnas.87.24.9568. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 27.Kuriyama S., Mitoro A., Tsujinoue H., Nakatani T., Yoshiji H., Tsujimoto T., Yamazaki M., Fukui H. Particle-mediated gene transfer into murine livers using a newly developed gene gun. Gene Ther. 2000;7:1132–1136. doi: 10.1038/sj.gt.3301192. [DOI] [PubMed] [Google Scholar]
- 28.Wang S., Joshi S., Lu S. Delivery of DNA to skin by particle bombardment. Methods Mol. Biol. 2004;245:185–196. doi: 10.1385/1-59259-649-5:185. [DOI] [PubMed] [Google Scholar]
- 29.Kim D., Hoory T., Monie A., Ting J. P. Y., Hung C. F., Wu T. C. Enhancement of DNA vaccine potency through coadministration of CIITA DNA with DNA vaccines via gene gun. J. Immunol. 2008;180:7019–7027. doi: 10.4049/jimmunol.180.10.7019. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 30.Roberts L. K., Barr L. J., Fuller D. H., McMahon C. W., Leese P. T., Jones S. Clinical safety and efficacy of a powdered Hepatitis B nucleic acid vaccine delivered to the epidermis by a commercial prototype device. Vaccine. 2005;23:4867–4878. doi: 10.1016/j.vaccine.2005.05.026. [DOI] [PubMed] [Google Scholar]
- 31.Drape R. J., Macklin M. D., Barr L. J., Jones S., Haynes J. R., Dean H. J. Epidermal DNA vaccine for influenza is immunogenic in humans. Vaccine. 2006;24:4475–4481. doi: 10.1016/j.vaccine.2005.08.012. [DOI] [PubMed] [Google Scholar]
- 32.Cassaday R. D., Sondel P. M., King D. M., Macklin M. D., Gan J., Warner T. F., Zuleger C. L., Bridges A. J., Schalch H. G., Kim K. M., Hank J. A., Mahvi D. M., Albertini M. R. A phase I study of immunization using particle-mediated epidermal delivery of genes for gp100 and GM-CSF into uninvolved skin of melanoma patients. Clin. Cancer Res. 2007;13:540–549. doi: 10.1158/1078-0432.CCR-06-2039. [DOI] [PubMed] [Google Scholar]
- 33.Chang M. L., Chen J. C., Yeh C. T., Chang M. Y., Liang C. K., Chiu C. T., Lin D. Y., Liaw Y. F. Gene gun bombardment with DNA-coated gold particles is a potential alternative to hydrodynamics-based transfection for delivering genes into superficial hepatocytes. Hum. Gene Ther. 2008;19:391–395. doi: 10.1089/hum.2007.152. [DOI] [PubMed] [Google Scholar]
- 34.Neumann E., Schaefer-Ridder M., Wang Y., Hofschneider P. H. Gene transfer into mouse lymphoma cells by electroporation in high electric fields. EMBO J. 1982;1:841–845. doi: 10.1002/j.1460-2075.1982.tb01257.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 35.Favard C., Dean D. S., Rols M. P. Electrotransfer as a nonviral method of gene delivery. Curr. Gene Ther. 2007;7:67–77. doi: 10.2174/156652307779940207. [DOI] [PubMed] [Google Scholar]
- 36.Heller L. C., Ugen K., Heller R. Electroporation for targeted gene transfer. Expert Opin. Drug Deliv. 2005;2:255–268. doi: 10.1517/17425247.2.2.255. [DOI] [PubMed] [Google Scholar]
- 37.Heller R., Jaroszeski M., Atkin A., Moradpour D., Gilbert R., Wands J., Nicolau C. In vivo gene electroinjection and expression in rat liver. FEBS Lett. 1996;389:225–228. doi: 10.1016/0014-5793(96)00590-X. [DOI] [PubMed] [Google Scholar]
- 38.Suzuki T., Shin B. C., Fujikura K., Matsuzaki T., Takata K. Direct gene transfer into rat liver cells by in vivo electroporation. FEBS Lett. 1998;425:436–440. doi: 10.1016/S0014-5793(98)00284-1. [DOI] [PubMed] [Google Scholar]
- 39.Liu F., Huang L. Electric gene transfer to the liver following systemic administration of plasmid DNA. Gene Ther. 2002;9:1116–1119. doi: 10.1038/sj.gt.3301733. [DOI] [PubMed] [Google Scholar]
- 40.Sakai M., Nishikawa M., Thanaketpaisarn O., Yamashita F., Hashida M. Hepatocyte-targeted gene transfer by combination of vascularly delivered plasmid DNA and in vivo electroporation. Gene Ther. 2005;12:607–616. doi: 10.1038/sj.gt.3302435. [DOI] [PubMed] [Google Scholar]
- 41.Jaichandran S., Yap S. T. B., Khoo A. B. M., Ho L. P., Tien S. L., Kon O. L. In vivo liver electroporation: optimization and demonstration of therapeutic efficacy. Hum. Gene Ther. 2006;17:362–375. doi: 10.1089/hum.2006.17.362. [DOI] [PubMed] [Google Scholar]
- 42.Kim H. J., Greenleaf J. F., Kinnick R. R., Bronk J. T., Bolander M. E. Ultrasound-mediated transfection of mammalian cells. Hum. Gene Ther. 1996;7:1339–1346. doi: 10.1089/hum.1996.7.11-1339. [DOI] [PubMed] [Google Scholar]
- 43.Tata D. B., Dunn F., Tindall D. J. Selective clinical ultrasound signals mediate differential gene transfer and expression in two human prostate cancer cell lines: LnCap and PC-3. Biochem. Biophys. Res. Commun. 1997;234:64–67. doi: 10.1006/bbrc.1997.6578. [DOI] [PubMed] [Google Scholar]
- 44.Bao S., Thrall B. D., Miller D. L. Transfection of a reporter plasmid into cultured cells by sonoporation in vitro. Ultrasound Med. Biol. 1997;23:953–959. doi: 10.1016/S0301-5629(97)00025-2. [DOI] [PubMed] [Google Scholar]
- 45.Suzuki R., Takizawa T., Negishi Y., Utoguchi N., Maruyama K. Effective gene delivery with novel liposomal bubbles and ultrasonic destruction technology. Int. J. Pharm. 2008;354:49–55. doi: 10.1016/j.ijpharm.2007.10.034. [DOI] [PubMed] [Google Scholar]
- 46.Sonoda S., Tachibana K., Uchino E., Okubo A., Yamamoto M., Sakoda K., Hisatomi T., Sonoda K.-H., Negishi Y., Izumi Y., Takao S., Sakamoto T. Gene transfer to corneal epithelium and keratocytes mediated by ultrasound with microbubbles. Invest. Ophthalmol. Vis. Sci. 2006;47:558–564. doi: 10.1167/iovs.05-0889. [DOI] [PubMed] [Google Scholar]
- 47.Hynynen K., McDannold N., Martin H., Jolesz F. A., Vykhodtseva N. The threshold for brain damage in rabbits induced by bursts of ultrasound in the presence of an ultrasound contrast agent (Optison) Ultrasound Med. Biol. 2003;29:473–481. doi: 10.1016/S0301-5629(02)00741-X. [DOI] [PubMed] [Google Scholar]
- 48.Manome Y., Nakayama N., Nakayama K., Furuhata H. Insonation facilitates plasmid DNA transfection into the central nervous system and microbubbles enhance the effect. Ultrasound Med. Biol. 2005;31:693–702. doi: 10.1016/j.ultrasmedbio.2005.01.015. [DOI] [PubMed] [Google Scholar]
- 49.Shimamura M., Sato N., Taniyama Y., Yamamoto S., Endoh M., Kurinami H., Aoki M., Ogihara T., Kaneda Y., Morishita R. Development of efficient plasmid DNA transfer into adult rat central nervous system using microbubble-enhanced ultrasound. Gene Ther. 2004;11:1532–1539. doi: 10.1038/sj.gt.3302323. [DOI] [PubMed] [Google Scholar]
- 50.Shimamura M., Sato N., Taniyama Y., Kurinami H., Tanaka H., Takami T., Ogihara T., Tohyama M., Kaneda Y., Morishita R. Gene transfer into adult rat spinal cord using naked plasmid DNA and ultrasound microbubbles. J. Gene Med. 2005;7:1468–1474. doi: 10.1002/jgm.793. [DOI] [PubMed] [Google Scholar]
- 51.Sheyn D., Kimelman-Bleich N., Pelled G., Zilberman Y., Gazit D., Gazit Z. Ultrasound-based nonviral gene delivery induces bone formation in vivo. Gene Ther. 2007;15:257–266. doi: 10.1038/sj.gt.3303070. [DOI] [PubMed] [Google Scholar]
- 52.Guo H., Leung J. C. K., Chan L. Y. Y., Tsang A. W. L., Lam M. F., Lan H. Y., Lai K. N. Ultrasound-contrast agent mediated naked gene delivery in the peritoneal cavity of adult rat. Gene Ther. 2007;14:1712–1720. doi: 10.1038/sj.gt.3303040. [DOI] [PubMed] [Google Scholar]
- 53.Hou C. C., Wang W., Huang X. R., Fu P., Chen T. H., Sheikh-Hamad D., Lan H. Y. Ultrasound-microbubble-mediated gene transfer of inducible Smad7 blocks transforming growth factor-beta signaling and fibrosis in rat remnant kidney. Am. J. Pathol. 2005;166:761–771. doi: 10.1016/s0002-9440(10)62297-3. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 54.Chen S., Ding J., Bekeredjian R., Yang B., Shohet R. V., Johnston S. A., Hohmeier H. E., Newgard C. B., Grayburn P. A. Efficient gene delivery to pancreatic islets with ultrasonic microbubble destruction technology. Proc. Natl. Acad. Sci. U. S. A. 2006;103:8469–8474. doi: 10.1073/pnas.0602921103. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 55.Shen Z. P., Brayman A. A., Chen L., Miao C. H. Ultrasound with microbubbles enhances gene expression of plasmid DNA in the liver via intraportal delivery. Gene Ther. 2008;15:1147–1155. doi: 10.1038/gt.2008.51. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 56.Miao C. H., Brayman A. A., Loeb K. R., Ye P., Zhou L., Mourad P., Crum L. A. Ultrasound enhances gene delivery of human factor IX plasmid. Hum. Gene Ther. 2005;16:893–905. doi: 10.1089/hum.2005.16.893. [DOI] [PubMed] [Google Scholar]
- 57.Ohta S., Suzuki K., Tachibana K., Yamada G. Microbubble-enhanced sonoporation: efficient gene transduction technique for chick embryos. Genesis. 2003;37:91–101. doi: 10.1002/gene.10232. [DOI] [PubMed] [Google Scholar]
- 58.Nakashima M., Tachibana K., Iohara K., Ito M., Ishikawa M., Akamine A. Induction of reparative dentin formation by ultrasound-mediated gene delivery of growth/differentiation factor 11. Hum. Gene Ther. 2003;14:591–597. doi: 10.1089/104303403764539369. [DOI] [PubMed] [Google Scholar]
- 59.Suzuki R., Takizawa T., Negishi Y., Utoguchi N., Sawamura K., Tanaka K., Namai E., Oda Y., Matsumura Y., Maruyama K. Tumor specific ultrasound enhanced gene transfer in vivo with novel liposomal bubbles. J. Control. Release. 2008;125:137–144. doi: 10.1016/j.jconrel.2007.08.025. [DOI] [PubMed] [Google Scholar]
- 60.Taniyama Y., Tachibana K., Hiraoka K., Aoki M., Yamamoto S., Matsumoto K., Nakamura T., Ogihara T., Kaneda Y., Morishita R. Development of safe and efficient novel nonviral gene transfer using ultrasound: enhancement of transfection efficiency of naked plasmid DNA in skeletal muscle. Gene Ther. 2002;9:372–380. doi: 10.1038/sj.gt.3301678. [DOI] [PubMed] [Google Scholar]
- 61.Lu Q. L., Liang H. D., Partridge T., Blomley M. J. K. Microbubble ultrasound improves the efficiency of gene transduction in skeletal muscle in vivo with reduced tissue damage. Gene Ther. 2003;10:396–405. doi: 10.1038/sj.gt.3301913. [DOI] [PubMed] [Google Scholar]
- 62.Bekeredjian R., Chen S., Frenkel P. A., Grayburn P. A., Shohet R. V. Ultrasound-targeted microbubble destruction can repeatedly direct highly specific plasmid expression to the heart. Circulation. 2003;108:1022–1026. doi: 10.1161/01.CIR.0000084535.35435.AE. [DOI] [PubMed] [Google Scholar]
- 63.Bekeredjian R., Grayburn P. A., Shohet R. V. Use of ultrasound contrast agents for gene or drug delivery in cardiovascular medicine. J. Am. Coll. Cardiol. 2005;45:329–335. doi: 10.1016/j.jacc.2004.08.067. [DOI] [PubMed] [Google Scholar]
- 64.Huber P. E., Jenne J., Debus J., Wannenmacher M. F., Pfisterer P. A comparison of shock wave and sinusoidal-focused ultrasound-induced localized transfection of HeLa cells. Ultrasound Med Biol. 1999;25:1451–1457. doi: 10.1016/S0301-5629(99)00099-X. [DOI] [PubMed] [Google Scholar]
- 65.Liu F., Song Y., Liu D. Hydrodynamics-based transfection in animals by systemic administration of plasmid DNA. Gene Ther. 1999;6:1258–1266. doi: 10.1038/sj.gt.3300947. [DOI] [PubMed] [Google Scholar]
- 66.Zhang G., Budker V., Wolff J. A. High levels of foreign gene expression in hepatocytes after tail vein injections of naked plasmid DNA. Hum. Gene Ther. 1999;10:1735–1737. doi: 10.1089/10430349950017734. [DOI] [PubMed] [Google Scholar]
- 67.Zhang G., Gao X., Song Y. K., Vollmer R., Stolz D. B., Gasiorowski J. Z., Dean D. A., Liu D. Hydroporation as the mechanism of hydrodynamic delivery. Gene Ther. 2004;11:675–682. doi: 10.1038/sj.gt.3302210. [DOI] [PMC free article] [PubMed] [Google Scholar]
- 68.Suda T., Gao X., Stolz D. B., Liu D. Structural impact of hydrodynamic injection on mouse liver. Gene Ther. 2007;14:129–137. doi: 10.1038/sj.gt.3302865. [DOI] [PubMed] [Google Scholar]
- 69.Suda T., Liu D. Hydrodynamic gene delivery: its principles and applications. Mol. Ther. 2007;15:2063–2069. doi: 10.1038/sj.mt.6300314. [DOI] [PubMed] [Google Scholar]
- 70.Brunetti-Pierri N., Palmer D. J., Mane V., Finegold M., Beaudet A. L., Ng P. Increased hepatic transduction with reduced systemic dissemination and proinflammatory cytokines following hydrodynamic injection of helper-dependent adenoviral vectors. Mol. Ther. 2005;12:99–106. doi: 10.1016/j.ymthe.2005.03.001. [DOI] [PubMed] [Google Scholar]
- 71.Condiotti R., Curran M. A., Nolan G. P., Giladi H., Ketzinel-Gilad M., Gross E., Galun E. Prolonged liver-specific transgene expression by a non-primate lentiviral vector. Biochem. Biophys. Res. Commun. 2004;320:998–1006. doi: 10.1016/j.bbrc.2004.06.044. [DOI] [PubMed] [Google Scholar]
- 72.Arad U., Zeira E., El-Latif M. A., Mukherjee S., Mitchell L., Pappo O., Galun E., Oppenheim A. Liver-targeted gene therapy by SV40-based vectors using the hydrodynamic injection method. Hum. Gene Ther. 2005;16:361–371. doi: 10.1089/hum.2005.16.361. [DOI] [PubMed] [Google Scholar]
- 73.Herweijer H., Wolff J. A. Gene therapy progress and prospects: hydrodynamic gene delivery. Gene Ther. 2007;14:99–107. doi: 10.1038/sj.gt.3302891. [DOI] [PubMed] [Google Scholar]
- 74.Eastman S. J., Baskin K. M., Hodges B. L., Chu Q., Gates A., Dreusicke R., Anderson S., Scheule R. K. Development of catheter-based procedures for transducing the isolated rabbit liver with plasmid DNA. Hum. Gene Ther. 2002;13:2065–2077. doi: 10.1089/10430340260395910. [DOI] [PubMed] [Google Scholar]
- 75.Yoshino H., Hashizume K., Kobayashi E. Naked plasmid DNA transfer to the porcine liver using rapid injection with large volume. Gene Ther. 2006;13:1696–1702. doi: 10.1038/sj.gt.3302833. [DOI] [PubMed] [Google Scholar]
- 76.Alino S. F., Herrero M. J., Noguera I., Dasi F., Sanchez M. Pig liver gene therapy by noninvasive interventionist catheterism. Gene Ther. 2007;14:334–343. doi: 10.1038/sj.gt.3302873. [DOI] [PubMed] [Google Scholar]
- 77.Fabre J. W., Grehan A., Whitehorne M., Sawyer G. J., Dong X., Salehi S., Eckley L., Zhang X., Seddon M., Shah A. M., Davenport M., Rela M. Hydrodynamic gene delivery to the pig liver via an isolated segment of the inferior vena cava. Gene Ther. 2008;15:452–462. doi: 10.1038/sj.gt.3303079. [DOI] [PubMed] [Google Scholar]
- 78.Brunetti-Pierri N., Stapleton G. E., Palmer D. J., Zuo Y., Mane V. P., Finegold M. J., Beaudet A. L., Leland M. M., Mullins C. E., Ng P. Pseudo-hydrodynamic delivery of helper-dependent adenoviral vectors into non-human primates for liver-directed gene therapy. Mol Ther. 2007;15:732–740. doi: 10.1038/sj.mt.6300102. [DOI] [PubMed] [Google Scholar]
- 79.Suda T., Suda K., Liu D. Computer-assisted hydrodynamic gene delivery. Mol Ther. 2008;16:1098–1104. doi: 10.1038/mt.2008.66. [DOI] [PubMed] [Google Scholar]
- 80.Blaese R. M., Culver K. W., Miller A. D., Carter C. S., Fleisher T., Clerici M., Shearer G., Chang L., Chiang Y., Tolstoshev P., Greenblatt J. J., Rosenberg S. A., Klein H., Berger M., Mullen C. A., Ramsey W. J., Muul L., Morgan R. A., Anderson W. F. T lymphocyte-directed gene therapy for ADA-SCID: initial trial results after 4 years. Science. 1995;270:475–480. doi: 10.1126/science.270.5235.475. [DOI] [PubMed] [Google Scholar]