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. 1993 Mar;31(3):584–587. doi: 10.1128/jcm.31.3.584-587.1993

Genetic diversity within isolates of mutans streptococci recognized by an rRNA gene probe.

M Saarela 1, S Alaluusua 1, T Takei 1, S Asikainen 1
PMCID: PMC262824  PMID: 7681438

Abstract

A total of 79 clinical isolates of mutans streptococci and five laboratory strains representing serotypes c, d, e, f, and g were genotyped by a nonradioactive hybridization method with the rrnB rRNA operon of the Escherichia coli chromosome as a probe. The hybridization patterns of chromosomal DNA fragments obtained by digestion with restriction endonucleases HindIII, SmaI, and BamHI revealed genotypic heterogeneity among the serotypes and among isolates of the same serotype recovered from unrelated subjects. Diversity also existed among isolates obtained from a single subject. For 5 of 13 subjects studied, two or three genotypes within serotypes were found, while eight subjects harbored the same number of genotypes as serotypes. The data show that the method utilizing the rRNA gene probe is of value in determining the molecular epidemiology of isolates of mutans streptococci.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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