Abstract
In this work, we show that antibodies to baculovirus recombinant DNA polymerase of human cytomegalovirus are present in human sera during natural infection. Specific antibody was found to be of the immunoglobulin G class, produced at low titers only for a short period of time at the beginning of acute infection and not detectable in sera from patients with a convalescent or a latent phase of infection. These results were compared with those obtained with procaryotically expressed p52, the polymerase accessory protein.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chee M. S., Bankier A. T., Beck S., Bohni R., Brown C. M., Cerny R., Horsnell T., Hutchison C. A., 3rd, Kouzarides T., Martignetti J. A. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169. Curr Top Microbiol Immunol. 1990;154:125–169. doi: 10.1007/978-3-642-74980-3_6. [DOI] [PubMed] [Google Scholar]
- Ertl P. F., Powell K. L. Physical and functional interaction of human cytomegalovirus DNA polymerase and its accessory protein (ICP36) expressed in insect cells. J Virol. 1992 Jul;66(7):4126–4133. doi: 10.1128/jvi.66.7.4126-4133.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ertl P. F., Thomas M. S., Powell K. L. High level expression of DNA polymerases from herpesviruses. J Gen Virol. 1991 Jul;72(Pt 7):1729–1734. doi: 10.1099/0022-1317-72-7-1729. [DOI] [PubMed] [Google Scholar]
- Gönczöl E., Plotkin S. Progress in vaccine development for prevention of human cytomegalovirus infection. Curr Top Microbiol Immunol. 1990;154:255–274. doi: 10.1007/978-3-642-74980-3_10. [DOI] [PubMed] [Google Scholar]
- Landini M. P., Lazzarotto T., Ripalti A., Guan M. X., La Placa M. Antibody response to recombinant lambda gt11 fusion proteins in cytomegalovirus infection. J Clin Microbiol. 1989 Oct;27(10):2324–2327. doi: 10.1128/jcm.27.10.2324-2327.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Landini M. P., Re M. C., Mirolo G., Baldassarri B., La Placa M. Human immune response to cytomegalovirus structural polypeptides studied by immunoblotting. J Med Virol. 1985 Dec;17(4):303–311. doi: 10.1002/jmv.1890170403. [DOI] [PubMed] [Google Scholar]
- Landini M. P., Rossier E., Schmitz H. Antibodies to human cytomegalovirus structural polypeptides during primary infection. J Virol Methods. 1988 Dec;22(2-3):309–317. doi: 10.1016/0166-0934(88)90113-9. [DOI] [PubMed] [Google Scholar]
- Ripalti A., Landini M. P., Mocarski E. S., La Placa M. Identification and preliminary use of recombinant lambda gt11 fusion proteins in human cytomegalovirus diagnosis. J Gen Virol. 1989 May;70(Pt 5):1247–1251. doi: 10.1099/0022-1317-70-5-1247. [DOI] [PubMed] [Google Scholar]
- Rodgers B., Borysiewicz L., Mundin J., Graham S., Sissons P. Immunoaffinity purification of a 72K early antigen of human cytomegalovirus: analysis of humoral and cell-mediated immunity to the purified polypeptide. J Gen Virol. 1987 Sep;68(Pt 9):2371–2378. doi: 10.1099/0022-1317-68-9-2371. [DOI] [PubMed] [Google Scholar]
- Snydman D. R. Cytomegalovirus immunoglobulins in the prevention and treatment of cytomegalovirus disease. Rev Infect Dis. 1990 Sep-Oct;12 (Suppl 7):S839–S848. doi: 10.1093/clinids/12.supplement_7.s839. [DOI] [PubMed] [Google Scholar]