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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1993 May;31(5):1048–1054. doi: 10.1128/jcm.31.5.1048-1054.1993

Isolation of specific DNA fragments of Mycobacterium avium and their possible use in diagnosis.

D Thierry 1, V Vincent 1, F Clément 1, J L Guesdon 1
PMCID: PMC262878  PMID: 8501206

Abstract

We cloned and sequenced two DNA fragments (DT1 and DT6) from Mycobacterium avium serotype 2 for use in the identification of members of the M. avium-M. intracellulare complex (MAC). Reference strains of MAC belonging to serovars 1 to 28 were examined by using these DNA fragments as probes. The study revealed that the DT6 probe hybridized with DNAs from M. avium strains (serovars 1 to 6, 8 to 11, and 21), while the DT1 probe hybridized with DNAs from serovars 2, 3, 7, 12 to 20, and 23 to 25. DT1- and DT6-derived oligonucleotides were selected for use as primers in a polymerase chain reaction test. Amplification of the DT1 and DT6 sequences may provide the basis for a rapid and reliable assay for the detection of mycobacteria belonging to MAC.

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Selected References

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  1. Butler W. R., Thibert L., Kilburn J. O. Identification of Mycobacterium avium complex strains and some similar species by high-performance liquid chromatography. J Clin Microbiol. 1992 Oct;30(10):2698–2704. doi: 10.1128/jcm.30.10.2698-2704.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Böddinghaus B., Wolters J., Heikens W., Böttger E. C. Phylogenetic analysis and identification of different serovars of Mycobacterium intracellulare at the molecular level. FEMS Microbiol Lett. 1990 Jul;58(2):197–203. doi: 10.1111/j.1574-6968.1990.tb13978.x. [DOI] [PubMed] [Google Scholar]
  3. Daffé M., Lanéelle M. A., Asselineau C., Lévy-Frébault V., David H. Intérêt taxonomique des acides gras des mycobactéries: proposition d'une méthode d'analyse. Ann Microbiol (Paris) 1983 Sep-Oct;134B(2):241–256. [PubMed] [Google Scholar]
  4. Goto M., Oka S., Okuzumi K., Kimura S., Shimada K. Evaluation of acridinium-ester-labeled DNA probes for identification of Mycobacterium tuberculosis and Mycobacterium avium-Mycobacterium intracellulare complex in culture. J Clin Microbiol. 1991 Nov;29(11):2473–2476. doi: 10.1128/jcm.29.11.2473-2476.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Griffais R., André P. M., Thibon M. K-tuple frequency in the human genome and polymerase chain reaction. Nucleic Acids Res. 1991 Jul 25;19(14):3887–3891. doi: 10.1093/nar/19.14.3887. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hohn B., Collins J. A small cosmid for efficient cloning of large DNA fragments. Gene. 1980 Nov;11(3-4):291–298. doi: 10.1016/0378-1119(80)90069-4. [DOI] [PubMed] [Google Scholar]
  7. Hohn B., Murray K. Packaging recombinant DNA molecules into bacteriophage particles in vitro. Proc Natl Acad Sci U S A. 1977 Aug;74(8):3259–3263. doi: 10.1073/pnas.74.8.3259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lebrun L., Espinasse F., Poveda J. D., Vincent-Levy-Frebault V. Evaluation of nonradioactive DNA probes for identification of mycobacteria. J Clin Microbiol. 1992 Sep;30(9):2476–2478. doi: 10.1128/jcm.30.9.2476-2478.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lim S. D., Todd J., Lopez J., Ford E., Janda J. M. Genotypic identification of pathogenic Mycobacterium species by using a nonradioactive oligonucleotide probe. J Clin Microbiol. 1991 Jun;29(6):1276–1278. doi: 10.1128/jcm.29.6.1276-1278.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lévy-Frébault V. V., Portaels F. Proposed minimal standards for the genus Mycobacterium and for description of new slowly growing Mycobacterium species. Int J Syst Bacteriol. 1992 Apr;42(2):315–323. doi: 10.1099/00207713-42-2-315. [DOI] [PubMed] [Google Scholar]
  11. McFadden J. J., Butcher P. D., Chiodini R. J., Hermon-Taylor J. Determination of genome size and DNA homology between an unclassified Mycobacterium species isolated from patients with Crohn's disease and other mycobacteria. J Gen Microbiol. 1987 Jan;133(1):211–214. doi: 10.1099/00221287-133-1-211. [DOI] [PubMed] [Google Scholar]
  12. Musial C. E., Tice L. S., Stockman L., Roberts G. D. Identification of mycobacteria from culture by using the Gen-Probe Rapid Diagnostic System for Mycobacterium avium complex and Mycobacterium tuberculosis complex. J Clin Microbiol. 1988 Oct;26(10):2120–2123. doi: 10.1128/jcm.26.10.2120-2123.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Saito H., Tomioka H., Sato K., Tasaka H., Dawson D. J. Identification of various serovar strains of Mycobacterium avium complex by using DNA probes specific for Mycobacterium avium and Mycobacterium intracellulare. J Clin Microbiol. 1990 Aug;28(8):1694–1697. doi: 10.1128/jcm.28.8.1694-1697.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Saxegaard F., Baess I. Relationship between Mycobacterium avium, Mycobacterium paratuberculosis and "wood pigeon mycobacteria". Determinations by DNA-DNA hybridization. APMIS. 1988 Jan;96(1):37–42. [PubMed] [Google Scholar]
  15. Schaefer W. B. Serologic identification and classification of the atypical mycobacteria by their agglutination. Am Rev Respir Dis. 1965 Dec;92(6):85–93. doi: 10.1164/arrd.1965.92.6P2.85. [DOI] [PubMed] [Google Scholar]
  16. Thorel M. F., Krichevsky M., Lévy-Frébault V. V. Numerical taxonomy of mycobactin-dependent mycobacteria, emended description of Mycobacterium avium, and description of Mycobacterium avium subsp. avium subsp. nov., Mycobacterium avium subsp. paratuberculosis subsp. nov., and Mycobacterium avium subsp. silvaticum subsp. nov. Int J Syst Bacteriol. 1990 Jul;40(3):254–260. doi: 10.1099/00207713-40-3-254. [DOI] [PubMed] [Google Scholar]
  17. Tsang A. Y., Denner J. C., Brennan P. J., McClatchy J. K. Clinical and epidemiological importance of typing of Mycobacterium avium complex isolates. J Clin Microbiol. 1992 Feb;30(2):479–484. doi: 10.1128/jcm.30.2.479-484.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wayne L. G., Sramek H. A. Agents of newly recognized or infrequently encountered mycobacterial diseases. Clin Microbiol Rev. 1992 Jan;5(1):1–25. doi: 10.1128/cmr.5.1.1. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yoshimura H. H., Graham D. Y. Nucleic acid hybridization studies of mycobactin-dependent mycobacteria. J Clin Microbiol. 1988 Jul;26(7):1309–1312. doi: 10.1128/jcm.26.7.1309-1312.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]

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