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. 1993 May;31(5):1136–1142. doi: 10.1128/jcm.31.5.1136-1142.1993

Intracellular bacteria of porcine proliferative enteropathy: cultivation and maintenance in vitro.

G H Lawson 1, S McOrist 1, S Jasni 1, R A Mackie 1
PMCID: PMC262892  PMID: 8501214

Abstract

An obligate intracellular bacterium was isolated from the intestines of all 10 cases of porcine proliferative enteropathy from four different pig farms. The organism grew in a rat enterocyte cell line (IEC-18) and was maintained over 20 passages. The growth of the bacteria was assessed by immunostaining of cells exposed to infection. Infection was not associated with morphological cell change, and growth was confined to cells infected at the time of each transfer of infection and the progeny of these cells. The bacterium is a microaerophilic, cell dependent, curved or rod-shaped, gram-negative bacillus that multiplies freely in the enterocyte cytoplasm. Cell cultures containing the intracellular bacteria appear to be free of other microorganisms, including chlamydiae and viruses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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