Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1993 May;31(5):1342–1344. doi: 10.1128/jcm.31.5.1342-1344.1993

New latex reagent using monoclonal antibodies to capsular polysaccharide for reliable identification of both oxacillin-susceptible and oxacillin-resistant Staphylococcus aureus.

J M Fournier 1, A Bouvet 1, D Mathieu 1, F Nato 1, A Boutonnier 1, R Gerbal 1, P Brunengo 1, C Saulnier 1, N Sagot 1, B Slizewicz 1, et al.
PMCID: PMC262934  PMID: 8501240

Abstract

A new latex agglutination test (Pastorex Staph-Plus, Sanofi Diagnostics Pasteur), consisting of a mixture of latex particles coated with fibrinogen and immunoglobulin G for the detection of clumping factor and protein A and latex particles sensitized with monoclonal antibodies directed to Staphylococcus aureus serotype 5 and 8 capsular polysaccharides, was compared with three commercially available rapid agglutination methods for the identification of 220 isolates of S. aureus (61 oxacillin resistant) and 128 isolates of coagulase-negative staphylococci. The sensitivity for identification of S. aureus was high with the Pastorex Staph-Plus test (98.6%) compared with those of the other tests, which ranged from 91.8 to 84.5%. Test sensitivities for the identification of oxacillin-resistant S. aureus were as follows: Pastorex Staph-Plus, 95.1%; Pastorex Staph, 73.8%; Staphyslide, 72.1%; and StaphAurex, 49.2%.

Full text

PDF
1342

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aldridge K. E., Kogos C., Sanders C. V., Marier R. L. Comparison of rapid identification assays for Staphylococcus aureus. J Clin Microbiol. 1984 May;19(5):703–704. doi: 10.1128/jcm.19.5.703-704.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Arbeit R. D., Karakawa W. W., Vann W. F., Robbins J. B. Predominance of two newly described capsular polysaccharide types among clinical isolates of Staphylococcus aureus. Diagn Microbiol Infect Dis. 1984 Apr;2(2):85–91. doi: 10.1016/0732-8893(84)90002-6. [DOI] [PubMed] [Google Scholar]
  3. Berke A., Tilton R. C. Evaluation of rapid coagulase methods for the identification of Staphylococcus aureus. J Clin Microbiol. 1986 May;23(5):916–919. doi: 10.1128/jcm.23.5.916-919.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Boutonnier A., Nato F., Bouvet A., Lebrun L., Audurier A., Mazie J. C., Fournier J. M. Direct testing of blood culture for detection of the serotype 5 and 8 capsular polysaccharides of Staphylococcus aureus. J Clin Microbiol. 1989 May;27(5):989–993. doi: 10.1128/jcm.27.5.989-993.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dassy B., Stringfellow W. T., Lieb M., Fournier J. M. Production of type 5 capsular polysaccharide by Staphylococcus aureus grown in a semi-synthetic medium. J Gen Microbiol. 1991 May;137(5):1155–1162. doi: 10.1099/00221287-137-5-1155. [DOI] [PubMed] [Google Scholar]
  6. Essers L., Radebold K. Rapid and reliable identification of Staphylococcus aureus by a latex agglutination test. J Clin Microbiol. 1980 Nov;12(5):641–643. doi: 10.1128/jcm.12.5.641-643.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Flandrois J. P., Carret G. Study of the staphylococcal affinity to fibrinogen by passive hemagglutination: a tool for the Staphylococcus aureus identification. Zentralbl Bakteriol Mikrobiol Hyg A. 1981 Dec;251(2):171–176. [PubMed] [Google Scholar]
  8. Fournier J. M., Boutonnier A., Bouvet A. Staphylococcus aureus strains which are not identified by rapid agglutination methods are of capsular serotype 5. J Clin Microbiol. 1989 Jun;27(6):1372–1374. doi: 10.1128/jcm.27.6.1372-1374.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Fournier J. M., Bouvet A., Boutonnier A., Audurier A., Goldstein F., Pierre J., Bure A., Lebrun L., Hochkeppel H. K. Predominance of capsular polysaccharide type 5 among oxacillin-resistant Staphylococcus aureus. J Clin Microbiol. 1987 Oct;25(10):1932–1933. doi: 10.1128/jcm.25.10.1932-1933.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gregson D. B., Low D. E., Skulnick M., Simor A. E. Problems with rapid agglutination methods for identification of Staphylococcus aureus when Staphylococcus saprophyticus is being tested. J Clin Microbiol. 1988 Jul;26(7):1398–1399. doi: 10.1128/jcm.26.7.1398-1399.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Hochkeppel H. K., Braun D. G., Vischer W., Imm A., Sutter S., Staeubli U., Guggenheim R., Kaplan E. L., Boutonnier A., Fournier J. M. Serotyping and electron microscopy studies of Staphylococcus aureus clinical isolates with monoclonal antibodies to capsular polysaccharide types 5 and 8. J Clin Microbiol. 1987 Mar;25(3):526–530. doi: 10.1128/jcm.25.3.526-530.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Karakawa W. W., Fournier J. M., Vann W. F., Arbeit R., Schneerson R. S., Robbins J. B. Method for the serological typing of the capsular polysaccharides of Staphylococcus aureus. J Clin Microbiol. 1985 Sep;22(3):445–447. doi: 10.1128/jcm.22.3.445-447.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lindmark R., Movitz J., Sjöquist J. Extracellular protein A from a methicillin-resistant strain of Staphylococcus aureus. Eur J Biochem. 1977 Apr 15;74(3):623–628. doi: 10.1111/j.1432-1033.1977.tb11431.x. [DOI] [PubMed] [Google Scholar]
  14. Mathieu D., Picard V. Comparative evaluation of five agglutination techniques and a new miniaturized system for rapid identification of methicillin-resistant strains of Staphylococcus aureus. Zentralbl Bakteriol. 1991 Dec;276(1):46–53. doi: 10.1016/s0934-8840(11)80217-3. [DOI] [PubMed] [Google Scholar]
  15. Maxim P. E., Mathews H. L., Mengoli H. F. Single-tube mixed agglutination test for the detection of staphylococcal protein A. J Clin Microbiol. 1976 Nov;4(5):418–422. doi: 10.1128/jcm.4.5.418-422.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Poutrel B., Mendolia C., Sutra L., Fournier J. M. Reactivity of coagulase-negative staphylococci isolated from cow and goat milk with monoclonal antibodies to Staphylococcus aureus capsular polysaccharide types 5 and 8. J Clin Microbiol. 1990 Feb;28(2):358–360. doi: 10.1128/jcm.28.2.358-360.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sompolinsky D., Samra Z., Karakawa W. W., Vann W. F., Schneerson R., Malik Z. Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types. J Clin Microbiol. 1985 Nov;22(5):828–834. doi: 10.1128/jcm.22.5.828-834.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stringfellow W. T., Dassy B., Lieb M., Fournier J. M. Staphylococcus aureus growth and type 5 capsular polysaccharide production in synthetic media. Appl Environ Microbiol. 1991 Feb;57(2):618–621. doi: 10.1128/aem.57.2.618-621.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Wanger A. R., Morris S. L., Ericsson C., Singh K. V., LaRocco M. T. Latex agglutination-negative methicillin-resistant Staphylococcus aureus recovered from neonates: epidemiologic features and comparison of typing methods. J Clin Microbiol. 1992 Oct;30(10):2583–2588. doi: 10.1128/jcm.30.10.2583-2588.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Winblad S., Ericson C. Sensitized sheep red cells as a reactant for Staphylococcus aureus protein A. Methodology and epidemiology with special reference to weakly reacting methicillin-resistant strains. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Feb;81(1):150–156. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES