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. 1993 May;31(5):1358–1361. doi: 10.1128/jcm.31.5.1358-1361.1993

Evaluation of intraspecies genetic variation within the 16S rRNA gene of Mycoplasma hominis and detection by polymerase chain reaction.

A Blanchard 1, A Yáñez 1, K Dybvig 1, H L Watson 1, G Griffiths 1, G H Cassell 1
PMCID: PMC262938  PMID: 7684753

Abstract

Mycoplasma hominis is a heterogeneous species with DNA-DNA hybridization values ranging from 51 to 100%. We report here the sequencing of the 16S rRNA gene of a strain (183) that greatly differs from the type strain (PG21) of this species. Comparison of 16S rDNA sequences from these two strains showed limited differences, indicating that the two strains belong to the same rRNA species complex. Using these nucleotide sequence data, we established a rapid method for the detection of M. hominis by using polymerase chain reaction. This method was shown to be sensitive and specific when tested with reference strains and clinical isolates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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