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. 2008 Nov 3;113(4):929–935. doi: 10.1182/blood-2008-07-167775

Figure 6.

Figure 6

Deglycosylation of ADAMTS13 by PNGase F does not affect its VWF cleaving activity. (A) The molecular weight of the WT ADAMTS13 in HEK 293T cell–secretion decreased from 190 kDa in the buffer control (B) to 172 kDa after overnight incubation at 37°C with PNGase F in the presence (+) or absence (−) of EDTA, SDS, and β-mercaptoethanol (β-ME). (B) VWF cleaving activity, expressed in percent of NHP, of the ADAMTS13 protein assayed at varying concentrations after incubation with buffer (♦) or PNGase F (◇) in the absence of EDTA/SDS/β-ME. NHP: normal human plasma.