Figure 3. Structural effects of Q513 mutations in the dark and lit states monitored by circular dichroism.

The room-temperature far UV region (190 nm to 250 nm) CD spectra of the wildtype dark (black) and lit (grey) states, Q513L dark (orange) and lit (yellow) states, and the Q513N dark (dark blue) and lit (light blue) states are overlaid here for comparison. The differences between the dark and lit states are more pronounced in the wildtype compared to the Q513 mutants.