Table 1.
Reaction conditions for real-time RT-PCR
| Target | Primer sequences (5′–3′) | Amplified cDNA (bp) | Published human sequence | % Identity between monkey and human sequence | Monkey amplified fragment sequence |
|---|---|---|---|---|---|
| tPA | |||||
| Forward | GCTACGTCTTTAAGGCG | 365 | NM_000930 | 96 | EU427935 |
| Reverse | GATGCGAAACTGAGGC | ||||
| uPA | |||||
| Forward | ACCACAACGACATTGC | 244 | NM_002658 | 97 | EU427936 |
| Reverse | GAGCCGTAGTAGTGGG | ||||
| PAI-1 | |||||
| Forward | GCCATTACTACGACATCCT | 347 | NM_000602 | 97 | EU427934 |
| Reverse | CGTGCCACTCTCGTTC | ||||
| β-Actin | |||||
| Forward | ATCCGCAAAGACCTGT | 270 | NM_001101 | 97 | AY765990 |
| Reverse | GTCCGCTAGAAGCAT |