Abstract
Of 20 blood specimens testing positive for cytomegalovirus antigen after immediate processing, 19 (95%) remained positive when kept at room temperature for 24 h before processing. Quantitative antigenemia decreased by an average of 44% after storage. Compared with acetone fixation, formaldehyde fixation showed improved readability, fewer artifacts, and a higher degree of sensitivity.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bein G., Bitsch A., Hoyer J., Kirchner H. The detection of human cytomegalovirus immediate early antigen in peripheral blood leucocytes. J Immunol Methods. 1991 Mar 21;137(2):175–180. doi: 10.1016/0022-1759(91)90022-8. [DOI] [PubMed] [Google Scholar]
- Boeckh M., Bowden R. A., Goodrich J. M., Pettinger M., Meyers J. D. Cytomegalovirus antigen detection in peripheral blood leukocytes after allogeneic marrow transplantation. Blood. 1992 Sep 1;80(5):1358–1364. [PubMed] [Google Scholar]
- Gerna G., Revello M. G., Percivalle E., Morini F. Comparison of different immunostaining techniques and monoclonal antibodies to the lower matrix phosphoprotein (pp65) for optimal quantitation of human cytomegalovirus antigenemia. J Clin Microbiol. 1992 May;30(5):1232–1237. doi: 10.1128/jcm.30.5.1232-1237.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gerna G., Zipeto D., Parea M., Revello M. G., Silini E., Percivalle E., Zavattoni M., Grossi P., Milanesi G. Monitoring of human cytomegalovirus infections and ganciclovir treatment in heart transplant recipients by determination of viremia, antigenemia, and DNAemia. J Infect Dis. 1991 Sep;164(3):488–498. doi: 10.1093/infdis/164.3.488. [DOI] [PubMed] [Google Scholar]
- Koskinen P. K., Nieminen M. S., Mattila S. P., Häyry P. J., Lautenschlager I. T. The correlation between symptomatic CMV infection and CMV antigenemia in heart allograft recipients. Transplantation. 1993 Mar;55(3):547–551. doi: 10.1097/00007890-199303000-00017. [DOI] [PubMed] [Google Scholar]
- Landry M. L., Ferguson D. Comparison of quantitative cytomegalovirus antigenemia assay with culture methods and correlation with clinical disease. J Clin Microbiol. 1993 Nov;31(11):2851–2856. doi: 10.1128/jcm.31.11.2851-2856.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Miller H., Rossier E., Milk R., Thomas C. Prospective study of cytomegalovirus antigenemia in allograft recipients. J Clin Microbiol. 1991 May;29(5):1054–1055. doi: 10.1128/jcm.29.5.1054-1055.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- The T. H., van der Bij W., van den Berg A. P., van der Giessen M., Weits J., Sprenger H. G., van Son W. J. Cytomegalovirus antigenemia. Rev Infect Dis. 1990 Sep-Oct;12 (Suppl 7):S734–S744. [PubMed] [Google Scholar]
- The T. H., van der Ploeg M., van den Berg A. P., Vlieger A. M., van der Giessen M., van Son W. J. Direct detection of cytomegalovirus in peripheral blood leukocytes--a review of the antigenemia assay and polymerase chain reaction. Transplantation. 1992 Aug;54(2):193–198. doi: 10.1097/00007890-199208000-00001. [DOI] [PubMed] [Google Scholar]
- van den Berg A. P., Klompmaker I. J., Haagsma E. B., Scholten-Sampson A., Bijleveld C. M., Schirm J., van der Giessen M., Slooff M. J., The T. H. Antigenemia in the diagnosis and monitoring of active cytomegalovirus infection after liver transplantation. J Infect Dis. 1991 Aug;164(2):265–270. doi: 10.1093/infdis/164.2.265. [DOI] [PubMed] [Google Scholar]
- van den Berg A. P., van Son W. J., Haagsma E. B., Klompmaker I. J., Tegzess A. M., Schirm J., Dijkstra G., van der Giessen M., Slooff M. J., The T. H. Prediction of recurrent cytomegalovirus disease after treatment with ganciclovir in solid-organ transplant recipients. Transplantation. 1993 Apr;55(4):847–851. doi: 10.1097/00007890-199304000-00031. [DOI] [PubMed] [Google Scholar]
- van den Berg A. P., van der Bij W., van Son W. J., Anema J., van der Giessen M., Schirm J., Tegzess A. M., The T. H. Cytomegalovirus antigenemia as a useful marker of symptomatic cytomegalovirus infection after renal transplantation--a report of 130 consecutive patients. Transplantation. 1989 Dec;48(6):991–995. doi: 10.1097/00007890-198912000-00019. [DOI] [PubMed] [Google Scholar]
- van der Bij W., Schirm J., Torensma R., van Son W. J., Tegzess A. M., The T. H. Comparison between viremia and antigenemia for detection of cytomegalovirus in blood. J Clin Microbiol. 1988 Dec;26(12):2531–2535. doi: 10.1128/jcm.26.12.2531-2535.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van der Giessen M., The T. H., van Son W. J. Cytomegalovirus antigenemia assay. J Clin Microbiol. 1991 Dec;29(12):2909–2910. doi: 10.1128/jcm.29.12.2909-2910.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]