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. 1994 Apr;32(4):912–917. doi: 10.1128/jcm.32.4.912-917.1994

C-reactive protein and alpha 1-acid glycoprotein levels in dogs infected with Ehrlichia canis.

Y Rikihisa 1, S Yamamoto 1, I Kwak 1, Z Iqbal 1, G Kociba 1, J Mott 1, W Chichanasiriwithaya 1
PMCID: PMC263162  PMID: 8027343

Abstract

To elucidate whether acute-phase protein responses occur in dogs infected with Ehrlichia canis, C-reactive protein (CRP) and alpha 1-acid glycoprotein (AAG) levels were serially measured in the plasma of five dogs experimentally inoculated with E. canis and 10 sham-inoculated or noninoculated control dogs. The CRP concentration was measured by a canine-specific capture enzyme-linked immunosorbent assay, and the AAG concentration was measured by a canine-specific radial immunodiffusion method. In all E. canis-inoculated dogs, a 3.3- to 6.5-fold increase in the plasma CRP concentration and a 1.9- to 8.6-fold increase in the plasma AAG concentration over the preinoculation level occurred at days 4 to 6 postexposure. Despite the persistence of E. canis and high antibody titers, both CRP and AAG concentrations gradually declined to preexposure levels by day 34 postexposure. E. canis-infected dogs had mild and transient clinical signs which resolved without treatment by day 14 postexposure. The CRP and AAG concentrations in control inoculated or nontreated dogs remained within the normal range throughout the experimental period. Of 12 dogs naturally infected with E. canis, 75% had greater than 50 micrograms of CRP per ml and 83% had greater than 500 micrograms of AAG per ml. All of these 12 dogs had chronic and severe clinical signs of canine ehrlichiosis. Thus, elevations in the levels of acute-phase proteins occur in both acute and chronic canine ehrlichiosis. Determination of CRP and AAG concentrations may help in assessing the severity of inflammatory damage in dogs with E. canis infections.

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Selected References

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  1. Belpaire F. M., De Rick A., Dello C., Fraeyman N., Bogaert M. G. Alpha 1-acid glycoprotein and serum binding of drugs in healthy and diseased dogs. J Vet Pharmacol Ther. 1987 Mar;10(1):43–48. doi: 10.1111/j.1365-2885.1987.tb00075.x. [DOI] [PubMed] [Google Scholar]
  2. Bennett M., Schmid K. Immunosuppression by human plasma alpha 1-acid glycoprotein: importance of the carbohydrate moiety. Proc Natl Acad Sci U S A. 1980 Oct;77(10):6109–6113. doi: 10.1073/pnas.77.10.6109. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Caspi D., Snel F. W., Batt R. M., Bennett D., Rutteman G. R., Hartman E. G., Baltz M. L., Gruys E., Pepys M. B. C-reactive protein in dogs. Am J Vet Res. 1987 Jun;48(6):919–921. [PubMed] [Google Scholar]
  4. Cheresh D. A., Haynes D. H., Distasio J. A. Interaction of an acute phase reactant, alpha 1-acid glycoprotein (orosomucoid), with the lymphoid cell surface: a model for non-specific immune suppression. Immunology. 1984 Mar;51(3):541–548. [PMC free article] [PubMed] [Google Scholar]
  5. Conner J. G., Eckersall P. D., Ferguson J., Douglas T. A. Acute phase response in the dog following surgical trauma. Res Vet Sci. 1988 Jul;45(1):107–110. [PubMed] [Google Scholar]
  6. Dello C. P., Belpaire F. M., De Rick A., Fraeyman N. H. Influence of inflammation on serum concentration, molecular heterogeneity and drug binding properties of canine alpha-1-acid glycoprotein. J Vet Pharmacol Ther. 1988 Mar;11(1):71–76. doi: 10.1111/j.1365-2885.1988.tb00123.x. [DOI] [PubMed] [Google Scholar]
  7. Dello C. P., Belpaire F. M., Kint J. A., Fraeyman N. H. Dog alpha-1-acid glycoprotein: purification and biochemical characterization. J Pharmacol Methods. 1987 Dec;18(4):335–345. doi: 10.1016/0160-5402(87)90065-9. [DOI] [PubMed] [Google Scholar]
  8. Dillman R. C., Coles E. H. A canine serum fraction analogous to human C-reactive protein. Am J Vet Res. 1966 Nov;27(121):1769–1775. [PubMed] [Google Scholar]
  9. Ewing S. A. Canine ehrlichiosis. Adv Vet Sci Comp Med. 1969;13:331–353. [PubMed] [Google Scholar]
  10. Kawahara M., Suto C., Rikihisa Y., Yamamoto S., Tsuboi Y. Characterization of ehrlichial organisms isolated from a wild mouse. J Clin Microbiol. 1993 Jan;31(1):89–96. doi: 10.1128/jcm.31.1.89-96.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kuehn N. F., Gaunt S. D. Clinical and hematologic findings in canine ehrlichiosis. J Am Vet Med Assoc. 1985 Feb 15;186(4):355–358. [PubMed] [Google Scholar]
  12. Kushner I., Mackiewicz A. Acute phase proteins as disease markers. Dis Markers. 1987 Mar;5(1):1–11. [PubMed] [Google Scholar]
  13. Lainé E., Couderc R., Roch-Arveiller M., Vasson M. P., Giroud J. P., Raichvarg D. Modulation of human polymorphonuclear neutrophil functions by alpha 1-acid glycoprotein. Inflammation. 1990 Feb;14(1):1–9. doi: 10.1007/BF00914025. [DOI] [PubMed] [Google Scholar]
  14. Lindbäck S., Hellgren U., Julander I., Hansson L. O. The value of C-reactive protein as a marker of bacterial infection in patients with septicaemia/endocarditis and influenza. Scand J Infect Dis. 1989;21(5):543–549. doi: 10.3109/00365548909037883. [DOI] [PubMed] [Google Scholar]
  15. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  16. Morley J. J., Kushner I. Serum C-reactive protein levels in disease. Ann N Y Acad Sci. 1982;389:406–418. doi: 10.1111/j.1749-6632.1982.tb22153.x. [DOI] [PubMed] [Google Scholar]
  17. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  18. Ndung'u J. M., Eckersall P. D., Jennings F. W. Elevation of the concentration of acute phase proteins in dogs infected with Trypanosoma brucei. Acta Trop. 1991 Jun;49(2):77–86. doi: 10.1016/0001-706x(91)90055-o. [DOI] [PubMed] [Google Scholar]
  19. Reardon M. J., Pierce K. R. Acute experimental canine ehrlichiosis. I. Sequential reaction of the hemic and lymphoreticular systems. Vet Pathol. 1981 Jan;18(1):48–61. doi: 10.1177/030098588101800106. [DOI] [PubMed] [Google Scholar]
  20. Rikihisa Y., Ewing S. A., Fox J. C., Siregar A. G., Pasaribu F. H., Malole M. B. Analyses of Ehrlichia canis and a canine granulocytic Ehrlichia infection. J Clin Microbiol. 1992 Jan;30(1):143–148. doi: 10.1128/jcm.30.1.143-148.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Rikihisa Y. The tribe Ehrlichieae and ehrlichial diseases. Clin Microbiol Rev. 1991 Jul;4(3):286–308. doi: 10.1128/cmr.4.3.286. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Riley R. F., Zontine W. Further observations on the properties of dog C-reactive protein and the C-reactive protein response in the dog. J Lab Clin Med. 1972 Nov;80(5):698–703. [PubMed] [Google Scholar]
  23. Schofield K. P., Voulgari F., Gozzard D. I., Leyland M. J., Beeching N. J., Stuart J. C-reactive protein concentration as a guide to antibiotic therapy in acute leukaemia. J Clin Pathol. 1982 Aug;35(8):866–869. doi: 10.1136/jcp.35.8.866. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Walker J. S., Rundquist J. D., Taylor R., Wilson B. L., Andrews M. R., Barck J., Hogge A. L., Jr, Huxsoll D. L., Hildebrandt P. K., Nims R. M. Clinical and clinicopathologic findings in tropical canine pancytopenia. J Am Vet Med Assoc. 1970 Jul 1;157(1):43–55. [PubMed] [Google Scholar]
  25. Yamamoto S., Tagata K., Nagahata H., Ishikawa Y., Morimatsu M., Naiki M. Isolation of canine C-reactive protein and characterization of its properties. Vet Immunol Immunopathol. 1992 Jan 31;30(4):329–339. doi: 10.1016/0165-2427(92)90103-w. [DOI] [PubMed] [Google Scholar]
  26. Zahedi K., Mortensen R. F. Macrophage tumoricidal activity induced by human C-reactive protein. Cancer Res. 1986 Oct;46(10):5077–5083. [PubMed] [Google Scholar]

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