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. 2001 Jan-Feb;7(1):49–56. doi: 10.3201/eid0701.700049

Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene.

S Pedraza-Díaz 1, C Amar 1, G L Nichols 1, J McLauchlin 1
PMCID: PMC2631669  PMID: 11266294

Abstract

We developed a sensitive nested polymerase chain reaction procedure for the Cryptosporidium oocyst wall protein (COWP) gene. Amplification and genotyping were successful in 95.2% of 1,680 fecal samples, 77.6% by the unnested and 17.6% by the nested COWP procedure. The COWP gene was amplified from 2,128 fecal samples: 71 from livestock animals and 2,057 from humans. This series included 706 cases from seven drinking water-associated outbreaks and 51 cases from five swimming pool-associated outbreaks, as well as 1,300 sporadic cases.

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Selected References

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