Abstract
We used 199 Corynebacterium diphtheriae isolated from 1995 to 1997 in Russia to evaluate the ability of random amplified polymorphic DNA (RAPD) to identify the unique clonal group that emerged there in 1990. Our data show that RAPD can reliably, reproducibly, and rapidly screen a large number of strains to identify the epidemic clonal group.
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Selected References
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- Nakao H., Popovic T. Use of random amplified polymorphic DNA for rapid molecular subtyping of Corynebacterium diphtheriae. Diagn Microbiol Infect Dis. 1998 Mar;30(3):167–172. doi: 10.1016/s0732-8893(97)00237-x. [DOI] [PubMed] [Google Scholar]
- Popovic T., Kombarova S. Y., Reeves M. W., Nakao H., Mazurova I. K., Wharton M., Wachsmuth I. K., Wenger J. D. Molecular epidemiology of diphtheria in Russia, 1985-1994. J Infect Dis. 1996 Nov;174(5):1064–1072. doi: 10.1093/infdis/174.5.1064. [DOI] [PubMed] [Google Scholar]
- Regnault B., Grimont F., Grimont P. A. Universal ribotyping method using a chemically labelled oligonucleotide probe mixture. Res Microbiol. 1997 Nov;148(8):649–659. doi: 10.1016/S0923-2508(99)80064-3. [DOI] [PubMed] [Google Scholar]
- Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. doi: 10.1128/aem.51.5.873-884.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]