Skip to main content
NCBI home page
Emerging Infectious Diseases logoLink to Emerging Infectious Diseases
. 2001 Jan-Feb;7(1):141–145. doi: 10.3201/eid0701.010121

Tracking Cryptosporidium parvum by sequence analysis of small double-stranded RNA.

L Xiao 1, J Limor 1, C Bern 1, A A Lal 1; Epidemic Working Group1
PMCID: PMC2631692  PMID: 11266306

Abstract

We sequenced a 173-nucleotide fragment of the small double-stranded viruslike RNA of Cryptosporidium parvum isolates from 23 calves and 38 humans. Sequence diversity was detected at 17 sites. Isolates from the same outbreak had identical double-stranded RNA sequences, suggesting that this technique may be useful for tracking Cryptosporidium infection sources.

Full Text

The Full Text of this article is available as a PDF (72.6 KB).

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bonnin A., Fourmaux M. N., Dubremetz J. F., Nelson R. G., Gobet P., Harly G., Buisson M., Puygauthier-Toubas D., Gabriel-Pospisil G., Naciri M. Genotyping human and bovine isolates of Cryptosporidium parvum by polymerase chain reaction-restriction fragment length polymorphism analysis of a repetitive DNA sequence. FEMS Microbiol Lett. 1996 Apr 1;137(2-3):207–211. doi: 10.1111/j.1574-6968.1996.tb08107.x. [DOI] [PubMed] [Google Scholar]
  2. Centers for Disease Control and Prevention (CDC) Outbreak of cryptosporidiosis associated with a water sprinkler fountain--Minnesota, 1997. MMWR Morb Mortal Wkly Rep. 1998 Oct 16;47(40):856–860. [PubMed] [Google Scholar]
  3. Khramtsov N. V., Chung P. A., Dykstra C. C., Griffiths J. K., Morgan U. M., Arrowood M. J., Upton S. J. Presence of double-stranded RNAs in human and calf isolates of Cryptosporidium parvum. J Parasitol. 2000 Apr;86(2):275–282. doi: 10.1645/0022-3395(2000)086[0275:PODSRI]2.0.CO;2. [DOI] [PubMed] [Google Scholar]
  4. Khramtsov N. V., Woods K. M., Nesterenko M. V., Dykstra C. C., Upton S. J. Virus-like, double-stranded RNAs in the parasitic protozoan Cryptosporidium parvum. Mol Microbiol. 1997 Oct;26(2):289–300. doi: 10.1046/j.1365-2958.1997.5721933.x. [DOI] [PubMed] [Google Scholar]
  5. McLauchlin J., Pedraza-Díaz S., Amar-Hoetzeneder C., Nichols G. L. Genetic characterization of Cryptosporidium strains from 218 patients with diarrhea diagnosed as having sporadic cryptosporidiosis. J Clin Microbiol. 1999 Oct;37(10):3153–3158. doi: 10.1128/jcm.37.10.3153-3158.1999. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Morgan U. M., Constantine C. C., O'Donoghue P., Meloni B. P., O'Brien P. A., Thompson R. C. Molecular characterization of Cryptosporidium isolates from humans and other animals using random amplified polymorphic DNA analysis. Am J Trop Med Hyg. 1995 Jun;52(6):559–564. doi: 10.4269/ajtmh.1995.52.559. [DOI] [PubMed] [Google Scholar]
  7. Quiroz E. S., Bern C., MacArthur J. R., Xiao L., Fletcher M., Arrowood M. J., Shay D. K., Levy M. E., Glass R. I., Lal A. An outbreak of cryptosporidiosis linked to a foodhandler. J Infect Dis. 2000 Feb;181(2):695–700. doi: 10.1086/315279. [DOI] [PubMed] [Google Scholar]
  8. Sulaiman I. M., Xiao L., Yang C., Escalante L., Moore A., Beard C. B., Arrowood M. J., Lal A. A. Differentiating human from animal isolates of Cryptosporidium parvum. Emerg Infect Dis. 1998 Oct-Dec;4(4):681–685. doi: 10.3201/eid0404.980424. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Xiao L., Morgan U. M., Limor J., Escalante A., Arrowood M., Shulaw W., Thompson R. C., Fayer R., Lal A. A. Genetic diversity within Cryptosporidium parvum and related Cryptosporidium species. Appl Environ Microbiol. 1999 Aug;65(8):3386–3391. doi: 10.1128/aem.65.8.3386-3391.1999. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Emerging Infectious Diseases are provided here courtesy of Centers for Disease Control and Prevention

RESOURCES