Abstract
Diagnostic and epidemiologic virology laboratories have in large part traded conventional techniques of virus detection and identification for more rapid, novel, and sensitive molecular methods. By doing so, useful phenotypic characteristics are not being determined. We feel that the impact of this shift in emphasis has impaired studies of the biology of viruses. This position paper is a plea to the scientific and administrative communities to reconsider the importance of such information. We also suggest a revised paradigm for virus isolation and characterization and provide a rationale for accumulating biologic (phenotypic) information.
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Selected References
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- CASALS J. Procedures for identification of arthropod-borne viruses. Bull World Health Organ. 1961;24:723–734. [PMC free article] [PubMed] [Google Scholar]
- CLARKE D. H., CASALS J. Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Am J Trop Med Hyg. 1958 Sep;7(5):561–573. doi: 10.4269/ajtmh.1958.7.561. [DOI] [PubMed] [Google Scholar]
- Calisher C. H. Antigenic classification and taxonomy of flaviviruses (family Flaviviridae) emphasizing a universal system for the taxonomy of viruses causing tick-borne encephalitis. Acta Virol. 1988 Sep;32(5):469–478. [PubMed] [Google Scholar]
- Calisher C. H., Shope R. E., Brandt W., Casals J., Karabatsos N., Murphy F. A., Tesh R. B., Wiebe M. E. Proposed antigenic classification of registered arboviruses I. Togaviridae, Alphavirus. Intervirology. 1980;14(5-6):229–232. doi: 10.1159/000149190. [DOI] [PubMed] [Google Scholar]
- Chu Y. K., Rossi C., Leduc J. W., Lee H. W., Schmaljohn C. S., Dalrymple J. M. Serological relationships among viruses in the Hantavirus genus, family Bunyaviridae. Virology. 1994 Jan;198(1):196–204. doi: 10.1006/viro.1994.1022. [DOI] [PubMed] [Google Scholar]
- Hjelle B., Chavez-Giles F., Torrez-Martinez N., Yates T., Sarisky J., Webb J., Ascher M. Genetic identification of a novel hantavirus of the harvest mouse Reithrodontomys megalotis. J Virol. 1994 Oct;68(10):6751–6754. doi: 10.1128/jvi.68.10.6751-6754.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jia X. Y., Briese T., Jordan I., Rambaut A., Chi H. C., Mackenzie J. S., Hall R. A., Scherret J., Lipkin W. I. Genetic analysis of West Nile New York 1999 encephalitis virus. Lancet. 1999 Dec 4;354(9194):1971–1972. doi: 10.1016/s0140-6736(99)05384-2. [DOI] [PubMed] [Google Scholar]
- Lanciotti R. S., Roehrig J. T., Deubel V., Smith J., Parker M., Steele K., Crise B., Volpe K. E., Crabtree M. B., Scherret J. H. Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States. Science. 1999 Dec 17;286(5448):2333–2337. doi: 10.1126/science.286.5448.2333. [DOI] [PubMed] [Google Scholar]
- Levis S., Morzunov S. P., Rowe J. E., Enria D., Pini N., Calderon G., Sabattini M., St Jeor S. C. Genetic diversity and epidemiology of hantaviruses in Argentina. J Infect Dis. 1998 Mar;177(3):529–538. doi: 10.1086/514221. [DOI] [PubMed] [Google Scholar]
- Nichol S. T., Spiropoulou C. F., Morzunov S., Rollin P. E., Ksiazek T. G., Feldmann H., Sanchez A., Childs J., Zaki S., Peters C. J. Genetic identification of a hantavirus associated with an outbreak of acute respiratory illness. Science. 1993 Nov 5;262(5135):914–917. doi: 10.1126/science.8235615. [DOI] [PubMed] [Google Scholar]
- Powers A. M., Brault A. C., Tesh R. B., Weaver S. C. Re-emergence of Chikungunya and O'nyong-nyong viruses: evidence for distinct geographical lineages and distant evolutionary relationships. J Gen Virol. 2000 Feb;81(Pt 2):471–479. doi: 10.1099/0022-1317-81-2-471. [DOI] [PubMed] [Google Scholar]
- Powers A. M., Oberste M. S., Brault A. C., Rico-Hesse R., Schmura S. M., Smith J. F., Kang W., Sweeney W. P., Weaver S. C. Repeated emergence of epidemic/epizootic Venezuelan equine encephalitis from a single genotype of enzootic subtype ID virus. J Virol. 1997 Sep;71(9):6697–6705. doi: 10.1128/jvi.71.9.6697-6705.1997. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sanchez A., Ksiazek T. G., Rollin P. E., Miranda M. E., Trappier S. G., Khan A. S., Peters C. J., Nichol S. T. Detection and molecular characterization of Ebola viruses causing disease in human and nonhuman primates. J Infect Dis. 1999 Feb;179 (Suppl 1):S164–S169. doi: 10.1086/514282. [DOI] [PubMed] [Google Scholar]
- Schmaljohn C., Hjelle B. Hantaviruses: a global disease problem. Emerg Infect Dis. 1997 Apr-Jun;3(2):95–104. doi: 10.3201/eid0302.970202. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tesh R. B., Travassos Da Rosa A. P., Travassos Da Rosa J. S. Antigenic relationship among rhabdoviruses infecting terrestrial vertebrates. J Gen Virol. 1983 Jan;64(Pt 1):169–176. doi: 10.1099/0022-1317-64-1-169. [DOI] [PubMed] [Google Scholar]
- Travassos da Rosa A. P., Tesh R. B., Pinheiro F. P., Travassos da Rosa J. F., Peterson N. E. Characterization of eight new phlebotomus fever serogroup arboviruses (Bunyaviridae: Phlebovirus) from the Amazon region of Brazil. Am J Trop Med Hyg. 1983 Sep;32(5):1164–1171. doi: 10.4269/ajtmh.1983.32.1164. [DOI] [PubMed] [Google Scholar]
- Vincent M. J., Quiroz E., Gracia F., Sanchez A. J., Ksiazek T. G., Kitsutani P. T., Ruedas L. A., Tinnin D. S., Caceres L., Garcia A. Hantavirus pulmonary syndrome in Panama: identification of novel hantaviruses and their likely reservoirs. Virology. 2000 Nov 10;277(1):14–19. doi: 10.1006/viro.2000.0563. [DOI] [PubMed] [Google Scholar]
- Wang E., Ni H., Xu R., Barrett A. D., Watowich S. J., Gubler D. J., Weaver S. C. Evolutionary relationships of endemic/epidemic and sylvatic dengue viruses. J Virol. 2000 Apr;74(7):3227–3234. doi: 10.1128/jvi.74.7.3227-3234.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
