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. 2001 Jul-Aug;7(4):748–750. doi: 10.3201/eid0704.010428

Isolation and characterization of West Nile virus from the blood of viremic patients during the 2000 outbreak in Israel.

M Hindiyeh 1, L M Shulman 1, E Mendelson 1, L Weiss 1, Z Grossman 1, H Bin 1
PMCID: PMC2631769  PMID: 11585544

Abstract

We report the isolation of West Nile (WN) virus from four patient serum samples submitted for diagnosis during an outbreak of WN fever in Israel in 2000. Sequencing and phylogenetic analysis revealed two lineages, one closely related to a 1999 New York isolate and the other to a 1999 Russian isolate.

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Selected References

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  1. BERNKOPF H., LEVINE S., NERSON R. Isolation of West Nile virus in Israel. J Infect Dis. 1953 Nov-Dec;93(3):207–218. doi: 10.1093/infdis/93.3.207. [DOI] [PubMed] [Google Scholar]
  2. Berthet F. X., Zeller H. G., Drouet M. T., Rauzier J., Digoutte J. P., Deubel V. Extensive nucleotide changes and deletions within the envelope glycoprotein gene of Euro-African West Nile viruses. J Gen Virol. 1997 Sep;78(Pt 9):2293–2297. doi: 10.1099/0022-1317-78-9-2293. [DOI] [PubMed] [Google Scholar]
  3. Chowers M. Y., Lang R., Nassar F., Ben-David D., Giladi M., Rubinshtein E., Itzhaki A., Mishal J., Siegman-Igra Y., Kitzes R. Clinical characteristics of the West Nile fever outbreak, Israel, 2000. Emerg Infect Dis. 2001 Jul-Aug;7(4):675–678. doi: 10.3201/eid0704.010414. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cohen D., Zaide Y., Karasenty E., Schwarz M., LeDuc J. W., Slepon R., Ksiazek T. G., Shemer J., Green M. S. Prevalence of antibodies to West Nile fever, sandfly fever Sicilian, and sandfly fever Naples viruses in healthy adults in Israel. Public Health Rev. 1999;27(1-3):217–230. [PubMed] [Google Scholar]
  5. Coia G., Parker M. D., Speight G., Byrne M. E., Westaway E. G. Nucleotide and complete amino acid sequences of Kunjin virus: definitive gene order and characteristics of the virus-specified proteins. J Gen Virol. 1988 Jan;69(Pt 1):1–21. doi: 10.1099/0022-1317-69-1-1. [DOI] [PubMed] [Google Scholar]
  6. Flatau E., Kohn D., Daher O., Varsano N. West Nile fever encephalitis. Isr J Med Sci. 1981 Nov;17(11):1057–1059. [PubMed] [Google Scholar]
  7. Hall R. A., Burgess G. W., Kay B. H., Clancy P. Monoclonal antibodies to Kunjin and Kokobera viruses. Immunol Cell Biol. 1991 Feb;69(Pt 1):47–49. doi: 10.1038/icb.1991.7. [DOI] [PubMed] [Google Scholar]
  8. Lanciotti R. S., Kerst A. J., Nasci R. S., Godsey M. S., Mitchell C. J., Savage H. M., Komar N., Panella N. A., Allen B. C., Volpe K. E. Rapid detection of west nile virus from human clinical specimens, field-collected mosquitoes, and avian samples by a TaqMan reverse transcriptase-PCR assay. J Clin Microbiol. 2000 Nov;38(11):4066–4071. doi: 10.1128/jcm.38.11.4066-4071.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lanciotti R. S., Roehrig J. T., Deubel V., Smith J., Parker M., Steele K., Crise B., Volpe K. E., Crabtree M. B., Scherret J. H. Origin of the West Nile virus responsible for an outbreak of encephalitis in the northeastern United States. Science. 1999 Dec 17;286(5448):2333–2337. doi: 10.1126/science.286.5448.2333. [DOI] [PubMed] [Google Scholar]
  10. Lustig S., Halevy M., Fuchs P., Ben-Nathan D., Lachmi B. E., Kobiler D., Israeli E., Olshevsky U. Can West Nile virus outbreaks be controlled? Isr Med Assoc J. 2000 Oct;2(10):733–737. [PubMed] [Google Scholar]
  11. Savage H. M., Ceianu C., Nicolescu G., Karabatsos N., Lanciotti R., Vladimirescu A., Laiv L., Ungureanu A., Romanca C., Tsai T. F. Entomologic and avian investigations of an epidemic of West Nile fever in Romania in 1996, with serologic and molecular characterization of a virus isolate from mosquitoes. Am J Trop Med Hyg. 1999 Oct;61(4):600–611. doi: 10.4269/ajtmh.1999.61.600. [DOI] [PubMed] [Google Scholar]
  12. Shi P. Y., Kauffman E. B., Ren P., Felton A., Tai J. H., Dupuis A. P., 2nd, Jones S. A., Ngo K. A., Nicholas D. C., Maffei J. High-throughput detection of West Nile virus RNA. J Clin Microbiol. 2001 Apr;39(4):1264–1271. doi: 10.1128/JCM.39.4.1264-1271.2001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shulman L. M., Handsher R., Yang C. F., Yang S. J., Manor J., Vonsover A., Grossman Z., Pallansch M., Mendelson E., Kew O. M. Resolution of the pathways of poliovirus type 1 transmission during an outbreak. J Clin Microbiol. 2000 Mar;38(3):945–952. doi: 10.1128/jcm.38.3.945-952.2000. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Weinberger M., Pitlik S. D., Gandacu D., Lang R., Nassar F., Ben David D., Rubinstein E., Izthaki A., Mishal J., Kitzes R. West Nile fever outbreak, Israel, 2000: epidemiologic aspects. Emerg Infect Dis. 2001 Jul-Aug;7(4):686–691. doi: 10.3201/eid0704.010416. [DOI] [PMC free article] [PubMed] [Google Scholar]

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