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Macrophage (MΦ) cytokine expression profile and secretion. (a) MΦ were stimulated for 24 hr with the indicated stimuli and reverse transcription–polymerase chain reaction was performed for the indicated suppressive/tolerogenic molecules and cytokines. (b) MΦ were stimulated for 24 hr, supernatants were collected and tumour necrosis factor-α was analysed by enzyme-linked immunosorbent assay.
