Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Infection and Immunity logoLink to Infection and Immunity
. 1984 Jul;45(1):82–86. doi: 10.1128/iai.45.1.82-86.1984

Biphasic effects of muramyl dipeptide or lipopolysaccharide on superoxide anion-generating activities of macrophages.

K Yagawa, M Kaku, Y Ichinose, S Nagao, A Tanaka, A Tomoda
PMCID: PMC263271  PMID: 6329960

Abstract

The superoxide anion (O2-)-generating activity of guinea pig macrophages stimulated by wheat germ agglutinin (WGA), immune complexes, or phorbol myristate acetate (PMA) was studied after short- and long-term exposures of the cells to muramyl dipeptide (MDP) or lipopolysaccharide (LPS). Neither MDP nor LPS alone induced O2- release in macrophages. Short-term (30 min) exposure to these agents caused the enhanced release of O2- in response to WGA or immune complexes, though the PMA-induced O2- generation was not affected. On the other hand, long-term exposure (more than 24 h) to MDP or LPS progressively enhanced O2- generation of the cells induced by WGA, immune complexes, or even PMA. These results suggest that the mechanism for O2- generation of macrophages stimulated by WGA or immune complexes differs from that stimulated by PMA and that the differences also exist between short- and long-term exposure to MDP or LPS.

Full text

PDF
82

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andrews P. C., Babior B. M. Endogenous protein phosphorylation by resting and activated human neutrophils. Blood. 1983 Feb;61(2):333–340. [PubMed] [Google Scholar]
  2. Baxter M. A., Leslie R. G., Reeves W. G. The stimulation of superoxide anion production in guinea-pig peritoneal macrophages and neutrophils by phorbol myristate acetate, opsonized zymosan and IgG2-containing soluble immune complexes. Immunology. 1983 Apr;48(4):657–665. [PMC free article] [PubMed] [Google Scholar]
  3. Castagna M., Takai Y., Kaibuchi K., Sano K., Kikkawa U., Nishizuka Y. Direct activation of calcium-activated, phospholipid-dependent protein kinase by tumor-promoting phorbol esters. J Biol Chem. 1982 Jul 10;257(13):7847–7851. [PubMed] [Google Scholar]
  4. Dewald B., Baggiolini M., Curnutte J. T., Babior B. M. Subcellular localization of the superoxide-forming enzyme in human neutrophils. J Clin Invest. 1979 Jan;63(1):21–29. doi: 10.1172/JCI109273. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Drath D. B., Karnovsky M. L. Superoxide production by phagocytic leukocytes. J Exp Med. 1975 Jan 1;141(1):257–262. doi: 10.1084/jem.141.1.257. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fantone J. C., Kinnes D. A. Prostaglandin E1 and prostaglandin I2 modulation of superoxide production by human neutrophils. Biochem Biophys Res Commun. 1983 Jun 15;113(2):506–512. doi: 10.1016/0006-291x(83)91754-0. [DOI] [PubMed] [Google Scholar]
  7. Goodwin B. J., Weinberg J. B. Receptor-mediated modulation of human monocyte, neutrophil, lymphocyte, and platelet function by phorbol diesters. J Clin Invest. 1982 Oct;70(4):699–706. doi: 10.1172/JCI110665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Johnston R. B., Jr, Godzik C. A., Cohn Z. A. Increased superoxide anion production by immunologically activated and chemically elicited macrophages. J Exp Med. 1978 Jul 1;148(1):115–127. doi: 10.1084/jem.148.1.115. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kakinuma K., Yamaguchi T., Kaneda M., Shimada K., Tomita Y., Chance B. A determination of H2O2 release by the treatment of human blood polymorphonuclear leukocytes with myristate. J Biochem. 1979 Jul;86(1):87–95. [PubMed] [Google Scholar]
  10. Kaku M., Yagawa K., Nagao S., Tanaka A. Enhanced superoxide anion release from phagocytes by muramyl dipeptide or lipopolysaccharide. Infect Immun. 1983 Feb;39(2):559–564. doi: 10.1128/iai.39.2.559-564.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Nakamura M., Baxter C. R., Masters B. S. Simultaneous demonstration of phagocytosis-connected oxygen consumption and corresponding NAD(P)H oxidase activity: direct evidence for NADPH as the predominant electron donor to oxygen in phagocytizing human neutrophils. Biochem Biophys Res Commun. 1981 Feb 12;98(3):743–751. doi: 10.1016/0006-291x(81)91175-x. [DOI] [PubMed] [Google Scholar]
  13. Niedel J. E., Kuhn L. J., Vandenbark G. R. Phorbol diester receptor copurifies with protein kinase C. Proc Natl Acad Sci U S A. 1983 Jan;80(1):36–40. doi: 10.1073/pnas.80.1.36. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pabst M. J., Hedegaard H. B., Johnston R. B., Jr Cultured human monocytes require exposure to bacterial products to maintain an optimal oxygen radical response. J Immunol. 1982 Jan;128(1):123–128. [PubMed] [Google Scholar]
  15. Pabst M. J., Johnston R. B., Jr Increased production of superoxide anion by macrophages exposed in vitro to muramyl dipeptide or lipopolysaccharide. J Exp Med. 1980 Jan 1;151(1):101–114. doi: 10.1084/jem.151.1.101. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Pick E., Mizel D. Role of transmethylation in the elicitation of an oxidative burst in macrophages. Cell Immunol. 1982 Sep 15;72(2):277–285. doi: 10.1016/0008-8749(82)90475-0. [DOI] [PubMed] [Google Scholar]
  17. Yagawa K., Itoh T., Tomoda A. Effect of transmethylation-reaction and increased levels of cAMP on superoxide generation of guinea-pig macrophages induced with wheat germ agglutinin and phorbor myristate. FEBS Lett. 1983 Apr 18;154(2):383–386. doi: 10.1016/0014-5793(83)80187-2. [DOI] [PubMed] [Google Scholar]
  18. Yagawa K., Okamura J. Role of adenosine deaminase in activation of macrophages. Infect Immun. 1981 Apr;32(1):394–397. doi: 10.1128/iai.32.1.394-397.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Yagawa K., Onoue K., Aida Y. Structural studies of Fc receptors. I. Binding properties, solubilization, and partial characterization of fc receptors of macrophages. J Immunol. 1979 Jan;122(1):366–373. [PubMed] [Google Scholar]
  20. Young J. D., Unkeless J. C., Kaback H. R., Cohn Z. A. Mouse macrophage Fc receptor for IgG gamma 2b/gamma 1 in artificial and plasma membrane vesicles functions as a ligand-dependent ionophore. Proc Natl Acad Sci U S A. 1983 Mar;80(6):1636–1640. doi: 10.1073/pnas.80.6.1636. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES