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. 1984 Jul;45(1):267–272. doi: 10.1128/iai.45.1.267-272.1984

Intestinal mucus trapping in the rapid expulsion of Trichinella spiralis by rats: induction and expression analyzed by quantitative worm recovery.

R G Bell, L S Adams, R W Ogden
PMCID: PMC263311  PMID: 6735469

Abstract

Rats were immunized with a Trichinella spiralis infection restricted by chemotherapy to the intestine (the T/M regime) or with a complete infection that resulted in the deposition of muscle larvae. After an oral challenge infection, rapid expulsion could be demonstrated in both groups within 20 min and with 100% recovery of the infectious dose from the stomachs and intestines of infected animals. Immune and nonimmune groups were distinguished by the large numbers of worms in the intestinal lumens of immune rats and large numbers of worms in the intestinal walls in nonimmune rats. Infectious larvae persisted in the stomach lumens for longer in the immune rats. There was no quantitative difference in worm distribution in the intestine during rapid expulsion in rats immunized with the T/M regime or those given a complete infection. However, in the complete infection group 69% of the luminal worms were trapped in mucus; this did not occur during rapid expulsion in rats immunized with the T/M regime. Mucus trapping was observed only when muscle larvae had matured to the infectious stage in muscle (28 days after the primary infection). Complete infection rats challenged at 14 or 21 days did not display significant mucus trapping of larvae in the intestinal lumen. We conclude that (i) mucus trapping is not essential for rapid expulsion and (ii) mucus trapping is produced by systemic exposure to target antigens of the infectious larvae.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alizadeh H., Wakelin D. Comparison of rapid expulsion of Trichinella spiralis in mice and rats. Int J Parasitol. 1982 Feb;12(1):65–73. doi: 10.1016/0020-7519(82)90097-2. [DOI] [PubMed] [Google Scholar]
  2. Bell R. G., Adams L. S., Gerb J. Strongyloides ratti: dissociation of the rat's protective immunity into systemic and intestinal components. Exp Parasitol. 1981 Dec;52(3):386–395. doi: 10.1016/0014-4894(81)90097-7. [DOI] [PubMed] [Google Scholar]
  3. Bell R. G., McGregor D. D., Adams L. S. Studies on the inhibition of rapid expulsion of Trichinella spiralis in rats. Int Arch Allergy Appl Immunol. 1982;69(1):73–80. doi: 10.1159/000233149. [DOI] [PubMed] [Google Scholar]
  4. Bell R. G., McGregor D. D., Adams L. S. Trichinella spiralis: characterization and strain distribution of rapid expulsion in inbred mice. Exp Parasitol. 1982 Jun;53(3):301–314. doi: 10.1016/0014-4894(82)90073-x. [DOI] [PubMed] [Google Scholar]
  5. Bell R. G., McGregor D. D., Despommier D. D. Trichinella spiralis: mediation of the intestinal component of protective immunity in the rat by multiple, phase-specific, antiparasitic responses. Exp Parasitol. 1979 Apr;47(2):140–157. doi: 10.1016/0014-4894(79)90068-7. [DOI] [PubMed] [Google Scholar]
  6. Bell R. G., McGregor D. D. Rapid expulsion of Trichinella spiralis: coinduction by using antigenic extracts of larvae and intestinal stimulation with an unrelated parasite. Infect Immun. 1980 Jul;29(1):194–199. doi: 10.1128/iai.29.1.194-199.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Bell R. G., McGregor D. D. Requirement for two discrete stimuli for induction of the intestinal rapid expulsion response against Trichinella spiralis in rats. Infect Immun. 1980 Jul;29(1):186–193. doi: 10.1128/iai.29.1.186-193.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Bell R. G., McGregor D. D. Trichinella spiralis: expression of rapid expulsion in rats exposed to an abbreviated enteral infection. Exp Parasitol. 1979 Aug;48(1):42–50. doi: 10.1016/0014-4894(79)90053-5. [DOI] [PubMed] [Google Scholar]
  9. Bell R. G., McGregor D. D. Trichinella spiralis: role of different life cycle phases in induction, maintenance, and expression of rapid expulsion in rats. Exp Parasitol. 1979 Aug;48(1):51–60. doi: 10.1016/0014-4894(79)90054-7. [DOI] [PubMed] [Google Scholar]
  10. Challacombe S. J., Tomasi T. B., Jr Systemic tolerance and secretory immunity after oral immunization. J Exp Med. 1980 Dec 1;152(6):1459–1472. doi: 10.1084/jem.152.6.1459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Despommier D., Aron L., Turgeon L. Trichinella spiralis: growth of the intracellular (muscle) larva. Exp Parasitol. 1975 Feb;37(1):108–116. doi: 10.1016/0014-4894(75)90058-2. [DOI] [PubMed] [Google Scholar]
  12. Elson C. O., Heck J. A., Strober W. T-cell regulation of murine IgA synthesis. J Exp Med. 1979 Mar 1;149(3):632–643. doi: 10.1084/jem.149.3.632. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kagnoff M. F. Induction and paralysis: a conceptual framework from which to examine the intestinal immune system. Gastroenterology. 1974 Jun;66(6):1240–1256. [PubMed] [Google Scholar]
  14. Korenaga M., Nawa Y., Mimori T., Tada I. Effects of preintestinal larval antigenic stimuli on the generation of intestinal immunity in Strongyloides ratti infection in rats. J Parasitol. 1983 Feb;69(1):78–82. [PubMed] [Google Scholar]
  15. Lake A. M., Bloch K. J., Sinclair K. J., Walker W. A. Anaphylactic release of intestinal goblet cell mucus. Immunology. 1980 Feb;39(2):173–178. [PMC free article] [PubMed] [Google Scholar]
  16. Mackenzie C. D., Jungery M., Taylor P. M., Ogilvie B. M. The in-vitro interaction of eosinophils, neutrophils, macrophages and mast cells with nematode surfaces in the presence of complement or antibodies. J Pathol. 1981 Feb;133(2):161–175. doi: 10.1002/path.1711330207. [DOI] [PubMed] [Google Scholar]
  17. Miller H. R., Huntley J. F., Wallace G. R. Immune exclusion and mucus trapping during the rapid expulsion of Nippostrongylus brasiliensis from primed rats. Immunology. 1981 Oct;44(2):419–429. [PMC free article] [PubMed] [Google Scholar]
  18. Rothberg R. M., Kraft S. C., Michalek S. M. Systemic immunity after local antigenic stimulation of the lymphoid tissue of the gastrointestinal tract. J Immunol. 1973 Dec;111(6):1906–1913. [PubMed] [Google Scholar]
  19. Russell D. A., Castro G. A. Physiological characterization of a biphasic immune response to Trichinella spiralis in the rat. J Infect Dis. 1979 Mar;139(3):304–312. doi: 10.1093/infdis/139.3.304. [DOI] [PubMed] [Google Scholar]

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