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. 1984 Sep;45(3):618–624. doi: 10.1128/iai.45.3.618-624.1984

Protective levels of human immunoglobulin G antibody to group B streptococcus type Ib.

K M Boyer, L S Kendall, C K Papierniak, M E Klegerman, S P Gotoff
PMCID: PMC263339  PMID: 6381309

Abstract

We studied the concentration of circulating human immunoglobulin G (IgG) antibody to the native capsular polysaccharide of group B streptococcus (GBS) type Ib necessary to protect mice against lethal challenge by laboratory and clinical GBS Ib strains. Antibody was measured by an enzyme-linked immunosorbent assay in which native polysaccharide antigen coupled to human serum albumin was used. The assay was standardized by a quantitative precipitation test, using native antigen and specific human IgG antibody purified by affinity chromatography. IgG anti-GBS Ib antibody level-protection curves for 90% lethal dose challenge of mice were sigmoidal. The curves of whole serum and affinity-chromatographed IgG anti-GBS Ib were superimposable. The serum concentrations of human antibody required for complete protection of mice varied with the infecting strain and ranged from 0.038 to 0.175 microgram/ml. Protective levels of human IgG anti-GBS Ib were lower than those we found previously for homologous protection against GBS Ia challenge (range, 0.25 to 1.0 microgram/ml).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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