Abstract
Incubation of Escherichia coli (10(4) organisms per ml) in cell-free rabbit lung lavage for 30 min at 37 degrees C resulted in a 70% reduction in colony counts on deoxycholate agar. A low-molecular-weight peptide (about 3,400 daltons), with zinc as a cofactor, was responsible for this activity. The peptide was isolated by Sephadex G-15 separation of lyophilized rabbit lung lavage which had been centrifuged to remove macrophages and suspended phospholipids and passed through a 10,000-dalton (pore size) membrane filter. Peptide activity against E. coli was inhibited by phosphate buffer but not by borate, Tris, or barbital buffer. Bacteria incubated in phosphate buffer and then washed in saline were resistant to peptide activity. Antibacterial activity was also inhibited when peptide-exposed bacteria were incubated in phosphate buffer before deoxycholate treatment. 32P-radiolabeled E. coli cells lost about 20% of their radiolabel after 15 min of incubation with peptide.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Coonrod J. D., Yoneda K. Detection and partial characterization of antibacterial factor(s) in alveolar lining material of rats. J Clin Invest. 1983 Jan;71(1):129–141. doi: 10.1172/JCI110741. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Green G. M., Jakab G. J., Low R. B., Davis G. S. Defense mechanisms of the respiratory membrane. Am Rev Respir Dis. 1977 Mar;115(3):479–514. doi: 10.1164/arrd.1977.115.3.479. [DOI] [PubMed] [Google Scholar]
- Jalowayski A. A., Giammona S. T. The interaction of bacteria with pulmonary surfactant. Am Rev Respir Dis. 1972 Feb;105(2):236–241. doi: 10.1164/arrd.1972.105.2.236. [DOI] [PubMed] [Google Scholar]
- LaForce F. M. Effect of alveolar lining material on phagocytic and bactericidal activity of lung macrophages against Staphylococcus aureau. J Lab Clin Med. 1976 Nov;88(5):691–699. [PubMed] [Google Scholar]
- Laforce F. M., Boose D. S. Sublethal damage of Escherichia coli by lung lavage. Am Rev Respir Dis. 1981 Dec;124(6):733–737. doi: 10.1164/arrd.1981.124.6.733. [DOI] [PubMed] [Google Scholar]
- MCGILVERY R. W., MOKRASCH L. C. Purification and properties of fructose-1, 6-diphosphatase. J Biol Chem. 1956 Aug;221(2):909–917. [PubMed] [Google Scholar]
- Patterson-Delafield J., Martinez R. J., Lehrer R. I. Microbicidal cationic proteins in rabbit alveolar macrophages: a potential host defense mechanism. Infect Immun. 1980 Oct;30(1):180–192. doi: 10.1128/iai.30.1.180-192.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Patterson-Delafield J., Szklarek D., Martinez R. J., Lehrer R. I. Microbicidal cationic proteins of rabbit alveolar macrophages: amino acid composition and functional attributes. Infect Immun. 1981 Feb;31(2):723–731. doi: 10.1128/iai.31.2.723-731.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sachs B. P., Stern C. M. Activity and characterization of a low molecular fraction present in human amniotic fluid with broad spectrum antibacterial activity. Br J Obstet Gynaecol. 1979 Feb;86(2):81–86. doi: 10.1111/j.1471-0528.1979.tb10572.x. [DOI] [PubMed] [Google Scholar]
- Schlievert P., Johnson W., Galask R. P. Bacterial growth inhibition by amniotic fluid. V. Phosphate-to-zinc ratio as a predictor of bacterial growth-inhibitory activity. Am J Obstet Gynecol. 1976 Aug 1;125(7):899–905. doi: 10.1016/0002-9378(76)90485-3. [DOI] [PubMed] [Google Scholar]
- Schlievert P., Johnson W., Galask R. P. Isolation of a low-molecular-weight antibacterial system from human amniotic fluid. Infect Immun. 1976 Nov;14(5):1156–1166. doi: 10.1128/iai.14.5.1156-1166.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]