Skip to main content
PMC home page
Infection and Immunity logoLink to Infection and Immunity
. 1984 Jan;43(1):189–194. doi: 10.1128/iai.43.1.189-194.1984

Piliated Bacteroides fragilis strains adhere to epithelial cells and are more sensitive to phagocytosis by human neutrophils than nonpiliated strains.

C Pruzzo, B Dainelli, M Ricchetti
PMCID: PMC263408  PMID: 6140223

Abstract

Fifteen unencapsulated B. fragilis strains isolated from human infections were examined for their capability to hemagglutinate erythrocytes of different species. Seven strains were found to hemagglutinate guinea pig and human (A,B,O) erythrocytes. This hemagglutination was resistant to treatment with D-mannose and several other sugars. Hemagglutinating strains were also capable of adhering to human epithelial cells and cultured human cell line (Intestine 407) and were 6- to 20-fold more adhesive than non-hemagglutinating strains. Pilus-like structures were found in negative-stained preparations on the hemagglutinating (and adhesive) strains but not on the others. Hemagglutinating and adhesive bacteria were 3- to 7-fold more sensitive to phagocytosis and 5- to 10-fold more sensitive to killing by human neutrophils than non-hemagglutinating ones.

Full text

PDF
189

Images in this article

192Image
on p.192

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bar-Shavit Z., Ofek I., Goldman R., Mirelman D., Sharon N. Mannose residues on phagocytes as receptors for the attachment of Escherichia coli and Salmonella typhi. Biochem Biophys Res Commun. 1977 Sep 9;78(1):455–460. doi: 10.1016/0006-291x(77)91276-1. [DOI] [PubMed] [Google Scholar]
  2. Beachey E. H. Bacterial adherence: adhesin-receptor interactions mediating the attachment of bacteria to mucosal surface. J Infect Dis. 1981 Mar;143(3):325–345. doi: 10.1093/infdis/143.3.325. [DOI] [PubMed] [Google Scholar]
  3. Blumenstock E., Jann K. Adhesion of piliated Escherichia coli strains to phagocytes: differences between bacteria with mannose-sensitive pili and those with mannose-resistant pili. Infect Immun. 1982 Jan;35(1):264–269. doi: 10.1128/iai.35.1.264-269.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bøyum A. Isolation of lymphocytes, granulocytes and macrophages. Scand J Immunol. 1976 Jun;Suppl 5:9–15. [PubMed] [Google Scholar]
  5. Chabanon G., Hartley C. L., Richmond M. H. In vitro attachment to a human cell line of Escherichia coli strains causing urinary-tract infection: occurrence of fimbriae (pili) in adhesive and non-adhesive strains. Ann Microbiol (Paris) 1982 May-Jun;133(3):357–369. [PubMed] [Google Scholar]
  6. Courtney H., Ofek I., Simpson W. A., Beachey E. H. Characterization of lipoteichoic acid binding to polymorphonuclear leukocytes of human blood. Infect Immun. 1981 May;32(2):625–631. doi: 10.1128/iai.32.2.625-631.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Densen P., Mandell G. L. Gonococcal interactions with polymorphonuclear neutrophils: importance of the phagosome for bactericidal activity. J Clin Invest. 1978 Dec;62(6):1161–1171. doi: 10.1172/JCI109235. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Duerden B. I., Collee J. G., Brown R., Deacon A. G., Holbrook W. P. A scheme for the identification of clinical isolates of Gram-negative anaerobic bacilli by conventional bacteriological tests. J Med Microbiol. 1980 May;13(2):231–245. doi: 10.1099/00222615-13-2-231. [DOI] [PubMed] [Google Scholar]
  9. Eisenstein B. I., Beachey E. H., Solomon S. S. Divergent effects of cyclic adenosine 3',5'-monophosphate on formation of type 1 fimbriae in different K-12 strains of Escherichia coli. J Bacteriol. 1981 Jan;145(1):620–623. doi: 10.1128/jb.145.1.620-623.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Eisenstein B. I. Phase variation of type 1 fimbriae in Escherichia coli is under transcriptional control. Science. 1981 Oct 16;214(4518):337–339. doi: 10.1126/science.6116279. [DOI] [PubMed] [Google Scholar]
  11. Gorbach S. L., Bartlett J. G. Anaerobic infections. 1. N Engl J Med. 1974 May 23;290(21):1177–1184. doi: 10.1056/NEJM197405232902106. [DOI] [PubMed] [Google Scholar]
  12. Kasper D. L., Onderdonk A. B., Polk B. F., Bartlett J. G. Surface antigens as virulence factors in infection with Bacteroides fragilis. Rev Infect Dis. 1979 Mar-Apr;1(2):278–290. doi: 10.1093/clinids/1.2.278. [DOI] [PubMed] [Google Scholar]
  13. Mangan D. F., Snyder I. S. Mannose-sensitive interaction of Escherichia coli with human peripheral leukocytes in vitro. Infect Immun. 1979 Nov;26(2):520–527. doi: 10.1128/iai.26.2.520-527.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Ofek I., Mirelman D., Sharon N. Adherence of Escherichia coli to human mucosal cells mediated by mannose receptors. Nature. 1977 Feb 17;265(5595):623–625. doi: 10.1038/265623a0. [DOI] [PubMed] [Google Scholar]
  15. Ofek I., Mosek A., Sharon N. Mannose-specific adherence of Escherichia coli freshly excreted in the urine of patients with urinary tract infections, and of isolates subcultured from the infected urine. Infect Immun. 1981 Dec;34(3):708–711. doi: 10.1128/iai.34.3.708-711.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Onderdonk A. B., Moon N. E., Kasper D. L., Bartlett J. G. Adherence of Bacteroides fragilis in vivo. Infect Immun. 1978 Mar;19(3):1083–1087. doi: 10.1128/iai.19.3.1083-1087.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Polk B. F., Kasper D. L. Bacteroides fragilis subspecies in clinical isolates. Ann Intern Med. 1977 May;86(5):569–571. doi: 10.7326/0003-4819-86-5-569. [DOI] [PubMed] [Google Scholar]
  18. Silverblatt F. J., Dreyer J. S., Schauer S. Effect of pili on susceptibility of Escherichia coli to phagocytosis. Infect Immun. 1979 Apr;24(1):218–223. doi: 10.1128/iai.24.1.218-223.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Slots J., Gibbons R. J. Attachment of Bacteroides melaninogenicus subsp. asaccharolyticus to oral surfaces and its possible role in colonization of the mouth and of periodontal pockets. Infect Immun. 1978 Jan;19(1):254–264. doi: 10.1128/iai.19.1.254-264.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Woods D. E., Straus D. C., Johanson W. G., Jr, Berry V. K., Bass J. A. Role of pili in adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells. Infect Immun. 1980 Sep;29(3):1146–1151. doi: 10.1128/iai.29.3.1146-1151.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES