Apoplastic invertases

Abstract

The mutualistic interaction of plants with arbuscular mycorrhizal (AM) fungi is characterized by an exchange of nutrients. The plant provides sugars in the form of hexoses to the heterotrophic fungus in return for phosphate as well as nitrogen, water, and micronutrients. Plant sucrose-cleaving enzymes are predicted to play a crucial role in hexose mobilization as these enzymes appear to be absent in the fungal partner. Here, recent findings concerning the function of plant apoplastic invertases in the AM symbiosis are discussed. Plants with modulated enzyme activity in roots and leaves provide additional insight on the complexity of the regulation of the AM interaction by apoplastic invertases as mycorrhization could be reduced or stimulated depending on the level of invertase activity and its tissue-specific expression.

Arbuscular mycorrhizas (AMs) are the most common type of mycorrhizas¹ and have been found in roots of more than 80% of the terrestrial plant species. AMs are intimate and, in most cases, mutualistic associations of plant roots and fungi of the phylum Glomeromycota.² They are crucial in the ecology and physiology of terrestrial plants, supporting plants under biotic (e.g., pathogen infection) or abiotic stress (e.g., nutrient or water deficiency and heavy metal stress). AMs have in common at least one feature with all interactions between plants and biotrophic microorganisms: the competition for carbohydrates. In the mutualistic association of AM the plant and the heterotrophic fungal partner have to share the carbohydrates which are assimilated by the plants' source leaves and translocated as sucrose to the sink tissue. Previous work revealed that AM-fungi are using intraradical hexoses, mainly glucose, as their carbon source.^3–5 To date no fungal sucrose-cleaving enzymes have been identified. After evaluating published data about AM-induced activity and/or transcript levels of plant sucrose-cleaving enzymes,^6–11 we suggest distinct roles for the different types of plant enzymes involved in providing the carbohydrate supply during mycorrhization: (i) Cytosolic invertases and sucrose-synthases are favored to supply the increased energy demand of (colonized) root cells to fulfill their higher metabolite activity and structural changes; (ii) vacuolar invertases might, particularly in early stages, release stored sugars for both the plant and the fungus, whereas (iii) extracellular, apoplast-located invertases might directly deliver hexoses to the apoplastic fungal structures and increase the sink function of the AM root where carbohydrate demand is high for both partners (i.e., during high colonization).

In addition to their function as carbon and energy sources, sugars also serve as important signals in the plant, regulating developmental as well as stress- and defense-related processes.¹² Particularly the apoplastic invertases are reported to be pathogenesis-related (PR) proteins.¹³ Thus, to avoid the activation of plant defense mechanisms in the mutualistic interaction, induction of these enzymes by the AM-fungus has to occur in a fine-tuned manner. Indeed, we could show that the induction of the apoplastic invertase LIN6, which is known to be inducible by a whole set of stress-related external stimuli,^14–16 is tightly controlled upon AM. In response to colonization, promoter activation and transcript accumulation of LIN6 was relatively low (compared to wounding of the root) and occurred in a cell-specific manner (near fungal structures and in the root phloem).¹¹

Interestingly, such a strictly controlled induction of apoplastic invertases is apparently sufficient for an optimal carbohydrate supply to the mycorrhizal root. Increased apoplastic invertase activity in the root accompanied by increased root hexose levels cannot improve mycorrhization.¹⁷ There must be other mechanisms overtaking the regulatory control of the symbiont.^18,19 In contrast, reduced apoplastic invertase activity in the root lowers root hexose levels and leads to a diminished mycorrhization, possibly by starvation effects on the fungus (Fig. 1A).¹⁷ This reveals the crucial impact of apoplastic invertases on the formation of an AM symbiosis.

Figure 1.

Regulation of arbuscular mycorrhization by modulated invertase activity in the plant apoplast. (A) A decreased mycorrhization can be achieved either by reduced activity of apoplastic invertase in the root or strongly increased activity in the leaf (up to 25-fold). In both cases, the root is characterized by an undersupply of carbohydrates. Moreover, the strong invertase activity in the source leaves results in sugar accumulation and activation of defense mechanisms in the leaves, that include the accumulation of PR transcript levels and phenolic compounds like chlorogenic acid isomers, scopolin and scopoletin.²² This increased defense status of the plant may contribute additionally to the inhibition of AM. (B) An increased mycorrhization cannot, however, be obtained by increased invertase activity in the root apoplast despite the higher root hexose levels. Instead, stimulated mycorrhization was observed in plants with slightly (2- to 4-fold) enhanced invertase activity in the leaf apoplast. Such plants showed an altered defense and hormone status in the root as characterized by a reduction in phenolic compounds (chlorogenic acid, scopolin and scopoletin), amines and some amino acids as well as increased abscisic acid levels. These changes are potentially triggered by slightly reduced sugar levels in the leaves.

Reduced mycorrhization is also exhibited by plants with drastically elevated levels of apoplastic invertase activity in the leaves.²⁰ The accumulation of hexoses in the source leaves does not only result in an undersupply of carbohydrates to the root, but also leads to an increased defense status.^20,21 Even if transcripts for the analyzed PR proteins were only detected in the leaves, we can not exclude the possibility that some constitutive defense mechanisms have been activated which inhibit mycorrhization (Fig. 1A).

There are limiting data, however, about a putative regulatory role for leaf apoplastic invertases on the root metabolism and/or biotic interactions of the root. However, there is recent evidence for a regulatory role of apoplastic invertases in leaves on the defense status of the root.²⁰ For the first time, we could demonstrate that slightly enhanced invertase activity in the leaf apoplast results in a strongly altered metabolite profile in the root, whereas less metabolic changes occurred in the leaves themselves. Some of the most significant metabolite changes observed in the root were reduced levels of amino acids, amines and phenolic compounds that are potentially involved in plant defense.^22–24 Moreover, the abscisic acid content of the root increases, which can positively affect the susceptibility to biotrophic fungi, including AM fungi.²⁵ These metabolic changes in the root result in a stimulated mycorrhization which could not be achieved by simply increasing the hexose levels as described above (Fig. 1B). Moreover, the steady-state levels of sugars in the roots of plants with slightly enhanced leaf invertase activity are not elevated (but are found to be slightly reduced), supporting a stronger regulatory role of plant secondary metabolism and/or hormone status than that of the pure nutritional benefit of the AM fungus. How such a regulation will also influence other biotic interactions remains to be elucidated.

In conclusion, we propose a more complex role for apoplastic invertases depending on their level of activity and tissue-specific expression in the plant: On the one hand, apoplastic invertases are efficiently regulating the carbon supply of the fungus by acting in the root. On the other hand, enhanced apoplastic invertase activity in the leaves can influence the defense status and the susceptibility of the root to AM fungi in two ways, leading either to a reduced or a stimulated colonization, depending on the level of enzyme activity.

Abbreviations

AM

Arbuscular mycorrhiza

PR

pathogenesis related

Addendum to: Schaarschmidt S, González MC, Roitsch T, Strack D, Sonnewald U, Hause B. Regulation of arbuscular mycorrhization by carbon. The symbiotic interaction cannot be improved by increased carbon availability accomplished by root-specifically enhanced invertase activity. Plant Physiol. 2007;143:1827–1840. doi: 10.1104/pp.106.096446.

Schaarschmidt S, Kopka J, Ludwig-Müller J, Hause B. Regulation of arbuscular mycorrhiza by apoplastic invertases: Increased invertase activity in the leaf apoplast affects the symbiotic interaction. Plant J. 2007;51:390–405. doi: 10.1111/j.1365-313X.2007.03150.x.