Figure 7.

Predicted secondary structures of Sup35NM. Gothic and italic letters indicate amino acids in the N and M domains of Sup35, respectively; underscores indicate six times repeat of PQGGYQQYN present in the N domain of Sup35. Secondary structures within Sup35NM predicted by the method of Chou and Fasman (1978) are notated below the corresponding amino acid sequence; circle, square and triangle represent amino acids composing α helix, β Sheet and coil, respectively. Numbers indicate positions where amino acid replacements were determined in the present study.