Figure 2.
The GFPC and GFPN104 proteins form morphologically distinct aggregates. Cells were grown to log phase in raffinose-containing media, and the expression of GFPC or GFPN104 proteins were induced by addition of galactose. Cells were harvested right before the galactose addition (0 hrs), and at the indicated time points after induction. GFP signals were visualized microscopically in live cells, which were grouped into four categories based on their GFP patterns: (i) no GFP signal, (ii) diffuse GFP signal, (iii) intermediate aggregates (arrowhead), and (iv) mature aggregates (arrow). For each sample at least 150 cells were examined. (A) Percentage of cells that have either intermediate or mature aggregates. (B) Percentage of cells that have mature aggregates. (C) Images of GFP and DAPI signals in formaldehyde-fixed cells that were harvested four hours after induction. **p value ≤ 0.005, ***p value ≤ 0.001