Abstract
An enzyme-linked immunosorbent assay was developed to enumerate Treponema pallidum cells. The assay could detect from 2 X 10(7) to 4 X 10(8) treponemes per ml. Reactive rabbit serum and goat anti-rabbit immunoglobulin G (peroxidase conjugate) were used in the assay. Optimum results were obtained when 2,2'-azino-di(ethylbenzthiazolinesulfonic acid) was used as the dye for the enzyme reaction and the reactions were allowed to run for 45 min. Interestingly, assays in which in vivo-cultivated T. pallidum was used produced lower absorbance values than those in which T. pallidum was cultivated in vitro.
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Selected References
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- Alderete J. F., Baseman J. B. Surface characterization of virulent Treponema pallidum. Infect Immun. 1980 Dec;30(3):814–823. doi: 10.1128/iai.30.3.814-823.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Alderete J. F., Baseman J. B. Surface-associated host proteins on virulent Treponema pallidum. Infect Immun. 1979 Dec;26(3):1048–1056. doi: 10.1128/iai.26.3.1048-1056.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Austin F. E., Barbieri J. T., Corin R. E., Grigas K. E., Cox C. D. Distribution of superoxide dismutase, catalase, and peroxidase activities among Treponema pallidum and other spirochetes. Infect Immun. 1981 Aug;33(2):372–379. doi: 10.1128/iai.33.2.372-379.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fieldsteel A. H., Cox D. L., Moeckli R. A. Cultivation of virulent Treponema pallidum in tissue culture. Infect Immun. 1981 May;32(2):908–915. doi: 10.1128/iai.32.2.908-915.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matthews H. M., Yang T. K., Jenkin H. M. Unique lipid composition of Treponema pallidum (Nichols virulent strain). Infect Immun. 1979 Jun;24(3):713–719. doi: 10.1128/iai.24.3.713-719.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morrison-Plummer J., Alderete J. F., Baseman J. B. Enzyme-linked immunosorbent assay for the detection of serum antibody to outer membrane proteins of Treponema pallidum. Br J Vener Dis. 1983 Apr;59(2):75–79. doi: 10.1136/sti.59.2.75. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sandok P. L., Knight S. T., Jenkin H. M. Examination of various cell culture techniques for co-incubation of virulent Treponema pallidum (Nichols I strain) under anaerobic conditions. J Clin Microbiol. 1976 Oct;4(4):360–371. doi: 10.1128/jcm.4.4.360-371.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]