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. 1984 Apr;44(1):182–187. doi: 10.1128/iai.44.1.182-187.1984

Specific immunoglobulin A antibodies to a peptide subunit sequence of bacterial cell wall peptidoglycan.

N Franken, P H Seidl, T Kuchenbauer, H J Kolb, K H Schleifer, L Weiss, K D Tympner
PMCID: PMC263490  PMID: 6423541

Abstract

Immunoglobulin A (IgA) antibodies in human sera with binding specificity for the C-terminal R-D-Ala-D-Ala sequence of the precursor peptide from bacterial cell wall peptidoglycan were detected by an enzyme-linked immunosorbent assay (ELISA). Specificity of the test system was proved by comparing the high binding of specific IgA to albumin-(D-Ala3) as an antigen with the failure to bind to albumin-(L-Ala3), by binding inhibition studies with L-Ala3, D-Ala3, or peptides with structural analogy to peptidoglycan peptide subunit peptides as inhibitors, and by excluding binding of peroxidase-labeled anti-human IgA to immunoglobulin classes others than IgA. Interference of rheumatoid factors of IgA class was excluded by an ELISA for assaying IgA-rheumatoid factor and by the fact that an IgA fraction essentially free of IgG and IgM was isolated from a serum reacting strongly positive in the ELISA for measuring specific IgA to the peptide subunit of peptidoglycan. This isolated IgA again exhibited binding specificity in the ELISA, thus corroborating the existence of specific IgA in human serum to the C-terminal R-D-Ala-D-Ala sequence of peptidoglycan precursor peptide. The existence of IgA antibodies with specificity for bacterial peptidoglycan was further proved by preadsorption of serum to peptidoglycans and subsequent measurement of specific IgA in the ELISA. Screening of human sera for IgA antibodies with specificity for R-D-Ala-D-Ala peptides revealed that specific antibodies directed against this sequence of bacterial cell wall peptidoglycan may be detected in several human sera.

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Selected References

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  1. Adam A., Petit J. F., Lefrancier P., Lederer E. Muramyl peptides. Chemical structure, biological activity and mechanism of action. Mol Cell Biochem. 1981 Dec 4;41:27–47. doi: 10.1007/BF00225295. [DOI] [PubMed] [Google Scholar]
  2. Bernstein D., Klapper D. G., Krause R. M. Use of radioimmunoassays to determine the concentration of streptococcal group-specific antibodies in rabbit antisera. J Immunol. 1975 Jan;114(1 Pt 1):59–63. [PubMed] [Google Scholar]
  3. Chetty C., Klapper D. G., Schwab J. H. Soluble peptidoglycan-polysaccharide fragments of the bacterial cell wall induce acute inflammation. Infect Immun. 1982 Dec;38(3):1010–1019. doi: 10.1128/iai.38.3.1010-1019.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Christensen P., Johansson B. G., Kronvall G. Interaction of streptococci with the Fc fragment of IgG. Acta Pathol Microbiol Scand C. 1976 Apr;84(2):73–76. doi: 10.1111/j.1699-0463.1976.tb00001.x. [DOI] [PubMed] [Google Scholar]
  5. Davis C. P., Houston C. W., Fader R. C., Goldblum R. M., Weaver E. A., Goldman A. S. Immunoglobulin A and secretory immunoglobulin A antibodies to purified type 1 Klebsiella pneumoniae pili in human colostrum. Infect Immun. 1982 Nov;38(2):496–501. doi: 10.1128/iai.38.2.496-501.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Eisenberg R., Fox A., Greenblatt J. J., Anderle S. K., Cromartie W. J., Schwab J. H. Measurement of bacterial cell wall in tissues by solid-phase radioimmunoassay: correlation of distribution and persistence with experimental arthritis in rats. Infect Immun. 1982 Oct;38(1):127–135. doi: 10.1128/iai.38.1.127-135.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fox A., Schwab J. H., Cochran T. Muramic acid detection in mammalian tissues by gas-liquid chromatography-mass spectrometry. Infect Immun. 1980 Aug;29(2):526–531. doi: 10.1128/iai.29.2.526-531.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Funakoshi S., Doi T., Nakajima T., Suyama T., Tokuda M. Antimicrobial effect of human serum IgA. Microbiol Immunol. 1982;26(3):227–239. doi: 10.1111/j.1348-0421.1982.tb00174.x. [DOI] [PubMed] [Google Scholar]
  9. Greenblatt J. J., Hunter N., Schwab J. H. Antibody response to streptococcal cell wall antigens associated with experimental arthritis in rats. Clin Exp Immunol. 1980 Dec;42(3):450–457. [PMC free article] [PubMed] [Google Scholar]
  10. Greenblatt J., Boackle R. J., Schwab J. H. Activation of the alternate complement pathway by peptidoglycan from streptococcal cell wall. Infect Immun. 1978 Jan;19(1):296–303. doi: 10.1128/iai.19.1.296-303.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Gripenberg M., Wafin F., Isomäki H., Linder E. A simple enzyme immunoassay for the demonstration of rheumatoid factor. J Immunol Methods. 1979;31(1-2):109–118. doi: 10.1016/0022-1759(79)90291-6. [DOI] [PubMed] [Google Scholar]
  12. Heymer B., Bernstein D., Schleifer K. H., Krause R. M. A radioactive hapten-binding assay for measuring antibodies to the pentapeptide determinant of peptidoglycan. J Immunol. 1975 Apr;114(4):1191–1196. [PubMed] [Google Scholar]
  13. Heymer B., Schleifer K. H., Read S., Zabriskie J. B., Krause R. M. Detection of antibodies to bacterial cell wall peptidoglycan in human sera. J Immunol. 1976 Jul;117(1):23–26. [PubMed] [Google Scholar]
  14. Killingsworth L. M., Savory J. Manual nephelometric methods for immunochemical determination of immunoglobulins IgG, IgA, and IgM in human serum. Clin Chem. 1972 Apr;18(4):335–339. [PubMed] [Google Scholar]
  15. Langone J. J., Boyle M. D., Borsos T. Studies on the interaction between protein A and immunoglobulin G. I. Effect of protein A on the functional activity of IgG. J Immunol. 1978 Jul;121(1):327–332. [PubMed] [Google Scholar]
  16. Melsom R., Harboe M., Duncan M. E., Bergsvik H. IgA and IgM antibodies against Mycobacterium leprae in cord sera and in patients with leprosy: an indicator of intrauterine infection in leprosy. Scand J Immunol. 1981 Oct;14(4):343–352. doi: 10.1111/j.1365-3083.1981.tb00574.x. [DOI] [PubMed] [Google Scholar]
  17. Pinku A., Haikin H., Friedman M. G., Sarov I. Detection of human cytomegalovirus- specific IgA antibodies in colostrum by enzyme-linked immunosorbent assay (ELISA). J Med Virol. 1982;9(2):111–116. doi: 10.1002/jmv.1890090205. [DOI] [PubMed] [Google Scholar]
  18. Rietschel E. T., Lüderitz O. Chemical structure of lipopolysaccharides and endotoxin immunity. Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(2-4):201–213. [PubMed] [Google Scholar]
  19. Rotta J. Biological activity of cellular components of group A streptococci in vivo. Curr Top Microbiol Immunol. 1969;48:63–101. doi: 10.1007/978-3-642-46163-7_4. [DOI] [PubMed] [Google Scholar]
  20. Rotta J. Endotoxin-like properties of the peptidoglycan. Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(2-4):230–244. [PubMed] [Google Scholar]
  21. Schleifer K. H. Chemical structure of the peptidoglycan, its modifiability and relation to the biological activity. Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(2-4):104–117. [PubMed] [Google Scholar]
  22. Schleifer K. H., Kandler O. Peptidoglycan types of bacterial cell walls and their taxonomic implications. Bacteriol Rev. 1972 Dec;36(4):407–477. doi: 10.1128/br.36.4.407-477.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Schleifer K. H., Krause R. M. The immunochemistry of peptidoglycan. I. The immunodominant site of the peptide subunit and the contribution of each of the amino acids to the binding properties of the peptides. J Biol Chem. 1971 Feb 25;246(4):986–993. [PubMed] [Google Scholar]
  24. Schleifer K. H., Krause R. M. The immunochemistry of peptidoglycan. Separation and characterization of antibodies to the glycan and to the peptide subunit. Eur J Biochem. 1971 Apr 30;19(4):471–478. doi: 10.1111/j.1432-1033.1971.tb01337.x. [DOI] [PubMed] [Google Scholar]
  25. Schleifer K. H., Seidl P. H. The immunochemistry of peptidoglycan. Antibodies against a synthetic immunogen cross-reacting with peptidoglycan. Eur J Biochem. 1974 Apr 16;43(3):509–519. doi: 10.1111/j.1432-1033.1974.tb03438.x. [DOI] [PubMed] [Google Scholar]
  26. Schopfer K., Douglas S. D., Wilkinson B. J. Immunoglobulin E antibodies against Staphylococcus aureus cell walls in the sera of patients with hyperimmunoglobulinemia E and recurrent staphylococcal infection. Infect Immun. 1980 Feb;27(2):563–568. doi: 10.1128/iai.27.2.563-568.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Seidl P. H., Schleifer K. H. Immunochemical studies with synthetic immunogens chemically related to peptidoglycan. Z Immunitatsforsch Exp Klin Immunol. 1975 Jul;149(2-4):157–164. [PubMed] [Google Scholar]
  28. Verbrugh H. A., Peters R., Rozenberg-Arska M., Peterson P. K., Verhoef J. Antibodies to cell wall peptidoglycan of Staphylococcus aureus in patients with serious staphylococcal infections. J Infect Dis. 1981 Jul;144(1):1–9. doi: 10.1093/infdis/144.1.1. [DOI] [PubMed] [Google Scholar]
  29. Ward J. B. Teichoic and teichuronic acids: biosynthesis, assembly, and location. Microbiol Rev. 1981 Jun;45(2):211–243. doi: 10.1128/mr.45.2.211-243.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Wheat L. J., Wilkinson B. J., Kohler R. B., White A. C. Antibody response to peptidoglycan during staphylococcal infections. J Infect Dis. 1983 Jan;147(1):16–22. doi: 10.1093/infdis/147.1.16. [DOI] [PubMed] [Google Scholar]
  31. Wicken A. J., Knox K. W. Lipoteichoic acids: a new class of bacterial antigen. Science. 1975 Mar 28;187(4182):1161–1167. doi: 10.1126/science.46620. [DOI] [PubMed] [Google Scholar]
  32. Zeiger A. R., Tuazon C. U., Sheagren J. N. Antibody levels to bacterial peptidoglycan in human sera during the time course of endocarditis and bacteremic infections caused by Staphylococcus aureus. Infect Immun. 1981 Sep;33(3):795–800. doi: 10.1128/iai.33.3.795-800.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. von Mayenburg J., Heymer B., Düngemann H., Schleifer K. H., Seidl P. H., Neiss A., Borelli S. Studies on bacterial hypersensitivity in man. Interrelationship between skin reactions to bacterial peptidoglycan and serum peptidoglycan antibody titers. Allergy. 1982 May;37(4):249–258. doi: 10.1111/j.1398-9995.1982.tb01907.x. [DOI] [PubMed] [Google Scholar]

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