Abstract
Lyt phenotypes of peritoneal exudate T lymphocytes from Listeria monocytogenes-immune mice were determined with respect to their capacity to confer protection upon local or systemic transfer. It is shown that, locally, Lyt 1 T cells provide protection, whereas only unselected native populations, containing adequate numbers of Lyt 123 T cells, were effective in transferring systemic protection. When Lyt 1 T cells were the crucial cell type (local transfer), admixture of Lyt 123-containing peritoneal exudate T-lymphocyte-enriched cells did not enhance protection, save for slight additive effects. Likewise, when Lyt 123-containing peritoneal exudate T lymphocyte-enriched cells were the crucial cell type (systemic transfer), admixture of various numbers of Lyt 1 cells was without marked effect. A change in Lyt phenotype from Lyt 123 to Lyt 1 paralleled by an increase of protective capacity upon local transfer was observed when sensitivity of early and late exudate T cells to anti-Lyt 2.2 antiserum plus complement treatment was investigated. The data suggest that (i) the propensity of specific T cells to enter exudates is associated with the Lyt 123 phenotype, (ii) the actual effector cell of antibacterial protection is an Lyt 1 T cell, and (iii) a developmental interrelation exists between Lyt 123 T cells and Lyt 1 T cells in T-cell-dependent protection of mice against L. monocytogenes.
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Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Cantor H., Boyse E. A. Functional subclasses of T lymphocytes bearing different Ly antigens. II. Cooperation between subclasses of Ly+ cells in the generation of killer activity. J Exp Med. 1975 Jun 1;141(6):1390–1399. doi: 10.1084/jem.141.6.1390. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cantor H., Boyse E. A. Functional subclasses of T-lymphocytes bearing different Ly antigens. I. The generation of functionally distinct T-cell subclasses is a differentiative process independent of antigen. J Exp Med. 1975 Jun 1;141(6):1376–1389. doi: 10.1084/jem.141.6.1376. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Cantor H., Boyse E. A. Lymphocytes as models for the study of mammalian cellular differentiation. Immunol Rev. 1977 Jan;33:105–124. doi: 10.1111/j.1600-065x.1977.tb00364.x. [DOI] [PubMed] [Google Scholar]
- Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
- Kaufmann S. H. Effective antibacterial protection induced by a Listeria monocytogenes-specific T cell clone and its lymphokines. Infect Immun. 1983 Mar;39(3):1265–1270. doi: 10.1128/iai.39.3.1265-1270.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaufmann S. H., Hahn H. Biological functions of t cell lines with specificity for the intracellular bacterium Listeria monocytogenes in vitro and in vivo. J Exp Med. 1982 Jun 1;155(6):1754–1765. doi: 10.1084/jem.155.6.1754. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaufmann S. H., Hahn H., Simon M. M., Röllinghoff M., Wagner H. Interleukin 2 induction in Lyt 1+ 23- T cells from Listeria monocytogenes-immune mice. Infect Immun. 1982 Sep;37(3):1292–1294. doi: 10.1128/iai.37.3.1292-1294.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kaufmann S. H., Hahn H., Simon M. M. T-cell subsets induced in Listeria monocytogenes-immune mice. Ly phenotypes of T cells interacting with macrophages in vitro. Scand J Immunol. 1982 Dec;16(6):539–542. doi: 10.1111/j.1365-3083.1982.tb00756.x. [DOI] [PubMed] [Google Scholar]
- Kaufmann S. H., Simon M. M., Hahn H. Specific Lyt 123 cells are involved in protection against Listeria monocytogenes and in delayed-type hypersensitivity to listerial antigens. J Exp Med. 1979 Oct 1;150(4):1033–1038. doi: 10.1084/jem.150.4.1033. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koster F. T., McGregor D. D., Mackaness G. B. The mediator of cellular immunity. II. Migration of immunologically committed lymphocytes into inflammatory exudates. J Exp Med. 1971 Feb 1;133(2):400–409. doi: 10.1084/jem.133.2.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ledbetter J. A., Rouse R. V., Micklem H. S., Herzenberg L. A. T cell subsets defined by expression of Lyt-1,2,3 and Thy-1 antigens. Two-parameter immunofluorescence and cytotoxicity analysis with monoclonal antibodies modifies current views. J Exp Med. 1980 Aug 1;152(2):280–295. doi: 10.1084/jem.152.2.280. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lin Y. L., Askonas B. A. Biological properties of an influenza A virus-specific killer T cell clone. Inhibition of virus replication in vivo and induction of delayed-type hypersensitivity reactions. J Exp Med. 1981 Aug 1;154(2):225–234. doi: 10.1084/jem.154.2.225. [DOI] [PMC free article] [PubMed] [Google Scholar]
- MACKANESS G. B. Cellular resistance to infection. J Exp Med. 1962 Sep 1;116:381–406. doi: 10.1084/jem.116.3.381. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McGregor D. D., Logie P. S. The mediator of cellular immunity. VII. Localization of sensitized lymphocytes in inflammatory exudates. J Exp Med. 1974 Jun 1;139(6):1415–1430. doi: 10.1084/jem.139.6.1415. [DOI] [PMC free article] [PubMed] [Google Scholar]
- North R. J., Mackaness G. B., Elliott R. W. The histogenesis of immunologically committed lymphocytes. Cell Immunol. 1972 Apr;3(4):680–694. doi: 10.1016/0008-8749(72)90130-x. [DOI] [PubMed] [Google Scholar]
- North R. J., Spitalny G. Inflammatory lymphocyte in cell-mediated antibacterial immunity: factors governing the accumulation of mediator T cells in peritoneal exudates. Infect Immun. 1974 Sep;10(3):489–498. doi: 10.1128/iai.10.3.489-498.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pavlov H., Hogarth M., McKenzie I. F., Cheers C. In vivo and in vitro effects of monoclonal antibody to Ly antigens on immunity to infection. Cell Immunol. 1982 Jul 15;71(1):127–138. doi: 10.1016/0008-8749(82)90502-0. [DOI] [PubMed] [Google Scholar]
- Shen F. W., McDougal J. S., Bard J., Cort S. P. Developmental and communicative interrelations of Ly123 and Ly1 cell sets. J Exp Med. 1980 Mar 1;151(3):566–572. doi: 10.1084/jem.151.3.566. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Varho M., Lehmann Grube F., Simon M. M. Effector T lymphocytes in lymphocytic choriomeningitis virus-infected mice. Cytolytic activity of Lyt-23 spleen cells in vitro does not correlate with elimination of infectious virus from spleens. J Exp Med. 1981 Apr 1;153(4):992–997. doi: 10.1084/jem.153.4.992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wagner H., Hardt C., Heeg K., Pfizenmaier K., Solbach W., Bartlett R., Stockinger H., Röllinghoff M. T-T cell interactions during cytotoxic T lymphocyte (CTL) responses: T cell derived helper factor (Interleukin 2) as a probe to analyze CTL responsiveness and thymic maturation of CTL progenitors. Immunol Rev. 1980;51:215–255. doi: 10.1111/j.1600-065x.1980.tb00323.x. [DOI] [PubMed] [Google Scholar]