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. 1984 May;44(2):434–438. doi: 10.1128/iai.44.2.434-438.1984

In vitro synthesis of the delta-lysin of Staphylococcus aureus.

K Y Lee, T H Birkbeck
PMCID: PMC263537  PMID: 6201445

Abstract

The stability of mRNA for the delta-lysin of Staphylococcus aureus was determined by measuring the residual lysin synthesis after inhibition of DNA-dependent RNA polymerase activity with rifampin. At the late logarithmic-early stationary phase of growth the delta-lysin mRNA was very stable, with a half-life of ca. 20 min. Total cellular RNA was extracted from S. aureus and translated with a modified Escherichia coli S-30 system; delta-lysin was identified amongst the translation products by immunoprecipitation and immunoabsorption. The delta-lysin synthesized in vitro was of a size similar to mature delta-lysin and did not require a signal sequence for secretion from the cell.

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