Figure 2.

Role of α4 and α5 in bone marrow homing of uncultured or ex vivo-expanded cord blood CD34⁺ cells. The presence of human CD34⁺ cells was determined in bone marrow (BM) harvested from NOD/SCID β₂m-null mice transplanted 16–24 hours previously with 500×10³ cord blood CD34⁺ cells before (A) or after (B) short-term culture. The function of α4 and α5 was assessed by incubating cells with P4C2 and P1D6 monoclonal prior to infusion in recipient mice antibodies, respectively. Control cells were treated with anti-CD34. p<0.05 compared to transplantation of control cells (n=3 experiments of two or three mice per group).