Figure 4.
RPA phosphorylation in a collection of checkpoint mutants. Western blot analysis of RPA2 from wild-type and checkpoint mutants (A) in exponentially growing (−) or HU-arrested (+) cells, (B) after mock (−) or ionizing radiation (+, 8 krad) exposure (IR), and (C) after mock (−) or UV radiation (+, 60 J/m2) exposure (UVR). TWY397 was the wild-type control in all three experiments. For the mec1-1 samples, TWY308 was used in A and DLY285 was used in B and C. Within each experiment, equal numbers of cells were analyzed.