Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1993 Apr;31(4):979–981. doi: 10.1128/jcm.31.4.979-981.1993

Evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to bovine leukemia virus in serum and milk.

V K Nguyen 1, R F Maes 1
PMCID: PMC263598  PMID: 8385156

Abstract

The results of examination of 375 bovine serum and 150 bovine milk samples for detection of bovine leukemia virus infection by the immunodiffusion technique were compared with those of an enzyme-linked immunosorbent assay (ELISA). It was concluded that the ELISA is another useful method for the detection of antibodies to bovine leukemia virus in serum and milk. The ELISA provides a quantitative result and has the advantage of being more sensitive and less time-consuming than the conventional immunodiffusion technique.

Full text

PDF
979

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baumgartener L. E., Olson C., Miller J. M., Van Der Maaten M. J. Survey for antibodies to leukemia (C-type) virus in cattle. J Am Vet Med Assoc. 1975 Feb 1;166(3):249–251. [PubMed] [Google Scholar]
  2. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  3. Castellano G. A., Hazzard G. T., Madden D. L., Sever J. L. Comparison of the enzyme-linked immunosorbent assay and the indirect hemagglutination test for detection of antibody to cytomegalovirus. J Infect Dis. 1977 Oct;136 (Suppl):S337–S340. doi: 10.1093/infdis/136.supplement_2.s337. [DOI] [PubMed] [Google Scholar]
  4. Daniel R. C. Induced hypocalcaemia in cows and sheep. III. Changes in plasma magnesium levels. Br Vet J. 1980 Jan-Feb;136(1):51–56. doi: 10.1016/s0007-1935(17)32387-4. [DOI] [PubMed] [Google Scholar]
  5. Levy D., Deshayes L., Parodi A. L., Levy J. P., Stephenson J. R., Devare S. G., Gilden R. V. Bovine leukemia virus specific antibodies among French cattle. II. Radioimmunoassay with the major structural protein (BLV p24). Int J Cancer. 1977 Oct 15;20(4):543–550. doi: 10.1002/ijc.2910200411. [DOI] [PubMed] [Google Scholar]
  6. Mammerickx M., Portetelle D., Kettmann P., Ghysdael J., Burny A., Dekegel D. Diagnostic tests of bovine leukemia: comparison between an hematological test and the serological diagnosis. Eur J Cancer. 1976 Jun;12(6):433–439. doi: 10.1016/0014-2964(76)90032-3. [DOI] [PubMed] [Google Scholar]
  7. Poli G., Balsari A., Toniolo A., Ponti W., Vacirca G. Microplate enzyme-linked immunosorbent assay for bovine leukemia virus antibody. J Clin Microbiol. 1981 Jan;13(1):46–48. doi: 10.1128/jcm.13.1.46-48.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Portetelle D., Bruck C., Mammerickx M., Burny A. Use of monoclonal antibody in an ELISA test for the detection of antibodies to bovine leukaemia virus. J Virol Methods. 1983 Jan;6(1):19–29. doi: 10.1016/0166-0934(83)90064-2. [DOI] [PubMed] [Google Scholar]
  9. Straub O. C. Diagnosis of enzootic bovine leukosis: a comparison of haematological and immunodiffusion tests. Res Vet Sci. 1978 Jul;25(1):13–15. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES