Abstract
Twenty-six strains of methicillin-resistant Staphylococcus aureus with different pulsed-field gel electrophoresis fingerprints were tested by random amplified polymorphic DNA assay with three primers, resulting in 15 to 20 different random amplified polymorphic DNA fingerprints. By summing the results for the three primers, the number of different fingerprints increased to 25, but two strains could not be differentiated. We conclude that pulsed-field gel electrophoresis remains the best method of typing methicillin-resistant S. aureus strains.
Full text
PDF



Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Allardet-Servent A., Bouziges N., Carles-Nurit M. J., Bourg G., Gouby A., Ramuz M. Use of low-frequency-cleavage restriction endonucleases for DNA analysis in epidemiological investigations of nosocomial bacterial infections. J Clin Microbiol. 1989 Sep;27(9):2057–2061. doi: 10.1128/jcm.27.9.2057-2061.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Archer G. L., Pennell E. Detection of methicillin resistance in staphylococci by using a DNA probe. Antimicrob Agents Chemother. 1990 Sep;34(9):1720–1724. doi: 10.1128/aac.34.9.1720. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boyce J. M. Methicillin-resistant Staphylococcus aureus. Detection, epidemiology, and control measures. Infect Dis Clin North Am. 1989 Dec;3(4):901–913. [PubMed] [Google Scholar]
- Brumfitt W., Hamilton-Miller J. Methicillin-resistant Staphylococcus aureus. N Engl J Med. 1989 May 4;320(18):1188–1196. doi: 10.1056/NEJM198905043201806. [DOI] [PubMed] [Google Scholar]
- Caetano-Anollés G., Bassam B. J., Gresshoff P. M. DNA amplification fingerprinting using very short arbitrary oligonucleotide primers. Biotechnology (N Y) 1991 Jun;9(6):553–557. doi: 10.1038/nbt0691-553. [DOI] [PubMed] [Google Scholar]
- Carles-Nurit M. J., Christophle B., Broche S., Gouby A., Bouziges N., Ramuz M. DNA polymorphisms in methicillin-susceptible and methicillin-resistant strains of Staphylococcus aureus. J Clin Microbiol. 1992 Aug;30(8):2092–2096. doi: 10.1128/jcm.30.8.2092-2096.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Costas M., Cookson B. D., Talsania H. G., Owen R. J. Numerical analysis of electrophoretic protein patterns of methicillin-resistant strains of Staphylococcus aureus. J Clin Microbiol. 1989 Nov;27(11):2574–2581. doi: 10.1128/jcm.27.11.2574-2581.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- De Buyser M. L., Morvan A., Grimont F., el Solh N. Characterization of Staphylococcus species by ribosomal RNA gene restriction patterns. J Gen Microbiol. 1989 Apr;135(4):989–999. doi: 10.1099/00221287-135-4-989. [DOI] [PubMed] [Google Scholar]
- Doebbeling B. N., Pfaller M. A., Hollis R. J., Boyken L. D., Pignatari A. C., Herwaldt L. A., Wenzel R. P. Restriction endonuclease analysis of Staphylococcus aureus plasmid DNA from three continents. Eur J Clin Microbiol Infect Dis. 1992 Jan;11(1):4–8. doi: 10.1007/BF01971263. [DOI] [PubMed] [Google Scholar]
- Goh S. H., Byrne S. K., Zhang J. L., Chow A. W. Molecular typing of Staphylococcus aureus on the basis of coagulase gene polymorphisms. J Clin Microbiol. 1992 Jul;30(7):1642–1645. doi: 10.1128/jcm.30.7.1642-1645.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Grimont F., Grimont P. A. Ribosomal ribonucleic acid gene restriction patterns as potential taxonomic tools. Ann Inst Pasteur Microbiol. 1986 Sep-Oct;137B(2):165–175. doi: 10.1016/s0769-2609(86)80105-3. [DOI] [PubMed] [Google Scholar]
- Gustaferro C. A., Persing D. H. Chemiluminescent universal probe for bacterial ribotyping. J Clin Microbiol. 1992 Apr;30(4):1039–1041. doi: 10.1128/jcm.30.4.1039-1041.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hall L. M., Jordens J. Z., Wang F. Methicillin-resistant Staphylococcus aureus from China characterized by digestion of total DNA with restriction enzymes. Epidemiol Infect. 1989 Aug;103(1):183–192. doi: 10.1017/s095026880003048x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hartstein A. I., Morthland V. H., Eng S., Archer G. L., Schoenknecht F. D., Rashad A. L. Restriction enzyme analysis of plasmid DNA and bacteriophage typing of paired Staphylococcus aureus blood culture isolates. J Clin Microbiol. 1989 Aug;27(8):1874–1879. doi: 10.1128/jcm.27.8.1874-1879.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ichiyama S., Ohta M., Shimokata K., Kato N., Takeuchi J. Genomic DNA fingerprinting by pulsed-field gel electrophoresis as an epidemiological marker for study of nosocomial infections caused by methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 1991 Dec;29(12):2690–2695. doi: 10.1128/jcm.29.12.2690-2695.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jordens J. Z., Hall L. M. Characterisation of methicillin-resistant Staphylococcus aureus isolates by restriction endonuclease digestion of chromosomal DNA. J Med Microbiol. 1988 Oct;27(2):117–123. doi: 10.1099/00222615-27-2-117. [DOI] [PubMed] [Google Scholar]
- Li W. H. Simple method for constructing phylogenetic trees from distance matrices. Proc Natl Acad Sci U S A. 1981 Feb;78(2):1085–1089. doi: 10.1073/pnas.78.2.1085. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Licitra C. M., Brooks R. G., Terry P. M., Shaw K. J., Hare R. S. Use of plasmid analysis and determination of aminoglycoside-modifying enzymes to characterize isolates from an outbreak of methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 1989 Nov;27(11):2535–2538. doi: 10.1128/jcm.27.11.2535-2538.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mazurier S. I., Wernars K. Typing of Listeria strains by random amplification of polymorphic DNA. Res Microbiol. 1992 Jun;143(5):499–505. doi: 10.1016/0923-2508(92)90096-7. [DOI] [PubMed] [Google Scholar]
- Musser J. M., Kapur V. Clonal analysis of methicillin-resistant Staphylococcus aureus strains from intercontinental sources: association of the mec gene with divergent phylogenetic lineages implies dissemination by horizontal transfer and recombination. J Clin Microbiol. 1992 Aug;30(8):2058–2063. doi: 10.1128/jcm.30.8.2058-2063.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- OEDING P. Serological typing of Staphylococci. Acta Pathol Microbiol Scand Suppl. 1952 Jun;93:356–365. [PubMed] [Google Scholar]
- Prevost G., Jaulhac B., Piemont Y. DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates. J Clin Microbiol. 1992 Apr;30(4):967–973. doi: 10.1128/jcm.30.4.967-973.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Prévost G., Pottecher B., Dahlet M., Bientz M., Mantz J. M., Piémont Y. Pulsed field gel electrophoresis as a new epidemiological tool for monitoring methicillin-resistant Staphylococcus aureus in an intensive care unit. J Hosp Infect. 1991 Apr;17(4):255–269. doi: 10.1016/0195-6701(91)90270-i. [DOI] [PubMed] [Google Scholar]
- Smith C. L., Cantor C. R. Purification, specific fragmentation, and separation of large DNA molecules. Methods Enzymol. 1987;155:449–467. doi: 10.1016/0076-6879(87)55030-3. [DOI] [PubMed] [Google Scholar]
- Sompolinsky D., Samra Z., Karakawa W. W., Vann W. F., Schneerson R., Malik Z. Encapsulation and capsular types in isolates of Staphylococcus aureus from different sources and relationship to phage types. J Clin Microbiol. 1985 Nov;22(5):828–834. doi: 10.1128/jcm.22.5.828-834.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thomson-Carter F. M., Carter P. E., Pennington T. H. Differentiation of staphylococcal species and strains by ribosomal RNA gene restriction patterns. J Gen Microbiol. 1989 Jul;135(7):2093–2097. doi: 10.1099/00221287-135-7-2093. [DOI] [PubMed] [Google Scholar]
- Wei M. Q., Wang F. U., Grubb W. B. Use of contour-clamped homogeneous electric field (CHEF) electrophoresis to type methicillin-resistant Staphylococcus aureus. J Med Microbiol. 1992 Mar;36(3):172–176. doi: 10.1099/00222615-36-3-172. [DOI] [PubMed] [Google Scholar]
- Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 1990 Dec 25;18(24):7213–7218. doi: 10.1093/nar/18.24.7213. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Williams J. G., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S. V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990 Nov 25;18(22):6531–6535. doi: 10.1093/nar/18.22.6531. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zuccarelli A. J., Roy I., Harding G. P., Couperus J. J. Diversity and stability of restriction enzyme profiles of plasmid DNA from methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 1990 Jan;28(1):97–102. doi: 10.1128/jcm.28.1.97-102.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]