Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1993 Apr;31(4):986–989. doi: 10.1128/jcm.31.4.986-989.1993

Comparison of nucleic acid hybridization and nucleic acid amplification using conserved sequences from the 5' noncoding region for detection of bovine viral diarrhea virus.

J F Ridpath 1, S R Bolin 1, J Katz 1
PMCID: PMC263600  PMID: 8385157

Abstract

Primers and probes derived from conserved sequences located in the 5' noncoding region of pestiviruses were evaluated for detection of bovine viral diarrhea virus. With these reagents, hybridization and polymerase chain reaction tests detected 62 of 90 and 90 of 90 bovine viral diarrhea virus isolates, respectively. A quick lysis method for preparing RNA for use in polymerase chain reaction amplification also was evaluated.

Full text

PDF
986

Images in this article

987Image
on p.987

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bolin S. R., Littledike E. T., Ridpath J. F. Serologic detection and practical consequences of antigenic diversity among bovine viral diarrhea viruses in a vaccinated herd. Am J Vet Res. 1991 Jul;52(7):1033–1037. [PubMed] [Google Scholar]
  2. Bolin S. R., Matthews P. J., Ridpath J. F. Methods for detection and frequency of contamination of fetal calf serum with bovine viral diarrhea virus and antibodies against bovine viral diarrhea virus. J Vet Diagn Invest. 1991 Jul;3(3):199–203. doi: 10.1177/104063879100300302. [DOI] [PubMed] [Google Scholar]
  3. Bolin S., Moennig V., Kelso Gourley N. E., Ridpath J. Monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhea virus into groups. Brief report. Arch Virol. 1988;99(1-2):117–123. doi: 10.1007/BF01311029. [DOI] [PubMed] [Google Scholar]
  4. Boye M., Kamstrup S., Dalsgaard K. Specific sequence amplification of bovine virus diarrhea virus (BVDV) and hog cholera virus and sequencing of BVDV nucleic acid. Vet Microbiol. 1991 Sep;29(1):1–13. doi: 10.1016/0378-1135(91)90106-p. [DOI] [PubMed] [Google Scholar]
  5. Brock K. V., Potgieter L. N. Detection of bovine viral diarrhea virus in serum from cattle by dot blot hybridization assay. Vet Microbiol. 1990 Sep;24(3-4):297–306. doi: 10.1016/0378-1135(90)90179-Y. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Colett M. S., Larson R., Gold C., Strick D., Anderson D. K., Purchio A. F. Molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. Virology. 1988 Jul;165(1):191–199. doi: 10.1016/0042-6822(88)90672-1. [DOI] [PubMed] [Google Scholar]
  7. Corapi W. V., Donis R. O., Dubovi E. J. Monoclonal antibody analyses of cytopathic and noncytopathic viruses from fatal bovine viral diarrhea virus infections. J Virol. 1988 Aug;62(8):2823–2827. doi: 10.1128/jvi.62.8.2823-2827.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Hafez S. M., Liess B. Studies on bovine viral diarrhea-mucosal disease virus. I. Cultural behaviour and antigenic relationship of some strains. Acta Virol. 1972 Sep;16(5):388–398. [PubMed] [Google Scholar]
  9. Hertig C., Pauli U., Zanoni R., Peterhans E. Detection of bovine viral diarrhea (BVD) virus using the polymerase chain reaction. Vet Microbiol. 1991 Jan;26(1-2):65–76. doi: 10.1016/0378-1135(91)90042-e. [DOI] [PubMed] [Google Scholar]
  10. Kelling C. L., Kennedy J. E., Rump K. K., Stine L. C., Paul P. S., Partridge J. E. Monitoring bovine viral diarrhea virus vaccines for adventitious virus, using T1 ribonuclease viral RNA oligonucleotide fingerprinting. Am J Vet Res. 1991 Aug;52(8):1237–1244. [PubMed] [Google Scholar]
  11. King A. A., Harkness J. W. Viral contamination of foetal bovine serum. Vet Rec. 1975 Jul 5;97(1):16–16. doi: 10.1136/vr.97.1.16. [DOI] [PubMed] [Google Scholar]
  12. Kwang J., Littledike E. T., Bolin S., Collett M. S. Efficiency of various cloned DNA probes for detection of bovine viral diarrhea viruses. Vet Microbiol. 1991 Aug 15;28(3):279–288. doi: 10.1016/0378-1135(91)90082-Q. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lewis T. L., Ridpath J. F., Bolin S. R., Berry E. S. Detection of BVD viruses using synthetic oligonucleotides. Arch Virol. 1991;117(3-4):269–278. doi: 10.1007/BF01310770. [DOI] [PubMed] [Google Scholar]
  14. Lopez O. J., Osorio F. A., Donis R. O. Rapid detection of bovine viral diarrhea virus by polymerase chain reaction. J Clin Microbiol. 1991 Mar;29(3):578–582. doi: 10.1128/jcm.29.3.578-582.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Meyers G., Rümenapf T., Thiel H. J. Molecular cloning and nucleotide sequence of the genome of hog cholera virus. Virology. 1989 Aug;171(2):555–567. doi: 10.1016/0042-6822(89)90625-9. [DOI] [PubMed] [Google Scholar]
  16. Molander C. W., Kniazeff A. J., Boone C. W., Paley A., Imagawa D. T. Isolation and characterization of viruses from fetal calf serum. In Vitro. 1971 Nov-Dec;7(3):168–173. doi: 10.1007/BF02617962. [DOI] [PubMed] [Google Scholar]
  17. Moormann R. J., Warmerdam P. A., van der Meer B., Schaaper W. M., Wensvoort G., Hulst M. M. Molecular cloning and nucleotide sequence of hog cholera virus strain Brescia and mapping of the genomic region encoding envelope protein E1. Virology. 1990 Jul;177(1):184–198. doi: 10.1016/0042-6822(90)90472-4. [DOI] [PubMed] [Google Scholar]
  18. Nuttall P. A., Luther P. D., Stott E. J. Viral contamination of bovine foetal serum and cell cultures. Nature. 1977 Apr 28;266(5605):835–837. doi: 10.1038/266835a0. [DOI] [PubMed] [Google Scholar]
  19. Osburn B. I., Clarke G. L., Stewart W. C., Sawyer M. Border disease-like syndrome in lambs: antibodies to hog cholera and bovine viral diarrhea viruses. J Am Vet Med Assoc. 1973 Nov 15;163(10):1165–1167. [PubMed] [Google Scholar]
  20. Peppel K., Baglioni C. A simple and fast method to extract RNA from tissue culture cells. Biotechniques. 1990 Dec;9(6):711–713. [PubMed] [Google Scholar]
  21. Potgieter L. N., Brock K. V. Detection of bovine viral diarrhea virus by spot hybridization with probes prepared from cloned cDNA sequences. J Vet Diagn Invest. 1989 Jan;1(1):29–33. doi: 10.1177/104063878900100110. [DOI] [PubMed] [Google Scholar]
  22. Potts B. J., Sawyer M., Shekarchi I. C., Wismer T., Huddleston D. Peroxidase-labeled primary antibody method for detection of pestivirus contamination in cell cultures. J Virol Methods. 1989 Oct;26(1):119–124. doi: 10.1016/0166-0934(89)90080-3. [DOI] [PubMed] [Google Scholar]
  23. Qi F., Ridpath J. F., Lewis T., Bolin S. R., Berry E. S. Analysis of the bovine viral diarrhea virus genome for possible cellular insertions. Virology. 1992 Jul;189(1):285–292. doi: 10.1016/0042-6822(92)90704-s. [DOI] [PubMed] [Google Scholar]
  24. Renard A., Guiot C., Schmetz D., Dagenais L., Pastoret P. P., Dina D., Martial J. A. Molecular cloning of bovine viral diarrhea viral sequences. DNA. 1985 Dec;4(6):429–438. doi: 10.1089/dna.1985.4.429. [DOI] [PubMed] [Google Scholar]
  25. Ridpath J. F., Bolin S. R. Hybridization analysis of genomic variability among isolates of bovine viral diarrhoea virus using cDNA probes. Mol Cell Probes. 1991 Aug;5(4):291–298. doi: 10.1016/0890-8508(91)90052-l. [DOI] [PubMed] [Google Scholar]
  26. Ridpath J. F., Lewis T. L., Bolin S. R., Berry E. S. Antigenic and genomic comparison between non-cytopathic and cytopathic bovine viral diarrhoea viruses isolated from cattle that had spontaneous mucosal disease. J Gen Virol. 1991 Mar;72(Pt 3):725–729. doi: 10.1099/0022-1317-72-3-725. [DOI] [PubMed] [Google Scholar]
  27. Roberts K. L., Collins J. K., Carman J., Blair C. D. Detection of cattle infected with bovine viral diarrhea virus using nucleic acid hybridization. J Vet Diagn Invest. 1991 Jan;3(1):10–15. doi: 10.1177/104063879100300103. [DOI] [PubMed] [Google Scholar]
  28. Rossi C. R., Bridgman C. R., Kiesel G. K. Viral contamination of bovine fetal lung cultures and bovine fetal serum. Am J Vet Res. 1980 Oct;41(10):1680–1681. [PubMed] [Google Scholar]
  29. Schroeder B. A., Balassu-Chan T. C. Specific sequence amplification of bovine viral diarrhoea virus nucleic acid. Arch Virol. 1990;111(3-4):239–246. doi: 10.1007/BF01311057. [DOI] [PubMed] [Google Scholar]
  30. Ward P., Misra V. Detection of bovine viral diarrhea virus, using degenerate oligonucleotide primers and the polymerase chain reaction. Am J Vet Res. 1991 Aug;52(8):1231–1236. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES