Skip to main content
NCBI home page
Infection and Immunity logoLink to Infection and Immunity
. 1984 Jun;44(3):565–568. doi: 10.1128/iai.44.3.565-568.1984

Proteins IA and IB exhibit different surface exposures and orientations in the outer membranes of Neisseria gonorrhoeae.

O Barrera, J Swanson
PMCID: PMC263626  PMID: 6427111

Abstract

Exposure of whole gonococci to proteinase K resulted in cleavage of protein I (P.I) of the organism in situ. P.I subunits in the P.IB group were cleaved into two membrane-associated fragments, whereas P.IA subunits were cleaved by proteinase K to yield a single membrane-associated fragment slightly smaller in apparent size than the intact P.IA subunit. These data suggest that P.IA and P.IB subunits are quite different in their surface exposures and orientations in the gonococcal outer membrane; P.IB subunits likely have both termini buried in the membrane, whereas P.IA subunits have one of their termini exposed on the surface of the organism.

Full text

PDF
565

Images in this article

566Image
on p.566
566Image
on p.566
566Image
on p.566
567Image
on p.567
567Image
on p.567

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Blake M. S., Gotschlich E. C. Gonococcal membrane proteins: speculation on their role in pathogenesis. Prog Allergy. 1983;33:298–313. [PubMed] [Google Scholar]
  2. Blake M. S., Gotschlich E. C., Swanson J. Effects of proteolytic enzymes on the outer membrane proteins of Neisseria gonorrhoeae. Infect Immun. 1981 Jul;33(1):212–222. doi: 10.1128/iai.33.1.212-222.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cannon J. G., Buchanan T. M., Sparling P. F. Confirmation of association of protein I serotype of Neisseria gonorrhoeae with ability to cause disseminated infection. Infect Immun. 1983 May;40(2):816–819. doi: 10.1128/iai.40.2.816-819.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Johnston K. H., Gotschlich E. C. Isolation and characterization of the outer membrane of Neisseria gonorrhoeae. J Bacteriol. 1974 Jul;119(1):250–257. doi: 10.1128/jb.119.1.250-257.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Johnston K. H., Holmes K. K., Gotschlich E. C. The serological classification of Neisseria gonorrhoeae. I. Isolation of the outer membrane complex responsible for serotypic specificity. J Exp Med. 1976 Apr 1;143(4):741–758. doi: 10.1084/jem.143.4.741. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Joiner K. A., Warren K. A., Brown E. J., Swanson J., Frank M. M. Studies on the mechanism of bacterial resistance to complement-mediated killing. IV. C5b-9 forms high molecular weight complexes with bacterial outer membrane constituents on serum-resistant but not on serum-sensitive Neisseria gonorrhoeae. J Immunol. 1983 Sep;131(3):1443–1451. [PubMed] [Google Scholar]
  7. Judd R. C. 125I-peptide mapping of protein III isolated from four strains of Neisseria gonorrhoeae. Infect Immun. 1982 Aug;37(2):622–631. doi: 10.1128/iai.37.2.622-631.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Lugtenberg B., Van Alphen L. Molecular architecture and functioning of the outer membrane of Escherichia coli and other gram-negative bacteria. Biochim Biophys Acta. 1983 Mar 21;737(1):51–115. doi: 10.1016/0304-4157(83)90014-x. [DOI] [PubMed] [Google Scholar]
  9. McDade R. L., Jr, Johnston K. H. Characterization of serologically dominant outer membrane proteins of Neisseria gonorrhoeae. J Bacteriol. 1980 Mar;141(3):1183–1191. doi: 10.1128/jb.141.3.1183-1191.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nakae T., Ishii J., Tokunaga M. Subunit structure of functional porin oligomers that form permeability channels in the other membrane of Escherichia coli. J Biol Chem. 1979 Mar 10;254(5):1457–1461. [PubMed] [Google Scholar]
  11. Nikaido H., Nakae T. The outer membrane of Gram-negative bacteria. Adv Microb Physiol. 1979;20:163–250. doi: 10.1016/s0065-2911(08)60208-8. [DOI] [PubMed] [Google Scholar]
  12. Sandstrom E. G., Chen K. C., Buchanan T. M. Serology of Neisseria gonorrhoeae: coagglutination serogroups WI and WII/III correspond to different outer membrane protein I molecules. Infect Immun. 1982 Nov;38(2):462–470. doi: 10.1128/iai.38.2.462-470.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Swanson J., Barrera O. Gonococcal pilus subunit size heterogeneity correlates with transitions in colony piliation phenotype, not with changes in colony opacity. J Exp Med. 1983 Nov 1;158(5):1459–1472. doi: 10.1084/jem.158.5.1459. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Swanson J. Colony opacity and protein II compositions of gonococci. Infect Immun. 1982 Jul;37(1):359–368. doi: 10.1128/iai.37.1.359-368.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Swanson J. Studies on gonococcus infection. XVIII. 125I-labeled peptide mapping of the major protein of the gonococcal cell wall outer membrane. Infect Immun. 1979 Mar;23(3):799–810. doi: 10.1128/iai.23.3.799-810.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Swanson J. Surface-exposed protein antigens of the gonococcal outer membrane. Infect Immun. 1981 Dec;34(3):804–816. doi: 10.1128/iai.34.3.804-816.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tam M. R., Buchanan T. M., Sandström E. G., Holmes K. K., Knapp J. S., Siadak A. W., Nowinski R. C. Serological classification of Neisseria gonorrhoeae with monoclonal antibodies. Infect Immun. 1982 Jun;36(3):1042–1053. doi: 10.1128/iai.36.3.1042-1053.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Young J. D., Blake M., Mauro A., Cohn Z. A. Properties of the major outer membrane protein from Neisseria gonorrhoeae incorporated into model lipid membranes. Proc Natl Acad Sci U S A. 1983 Jun;80(12):3831–3835. doi: 10.1073/pnas.80.12.3831. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Infection and Immunity are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES