Skip to main content
PMC home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 May;32(5):1211–1216. doi: 10.1128/jcm.32.5.1211-1216.1994

Development of species-specific enzyme-linked immunosorbent assay for diagnosis of Johne's disease in cattle.

P Vannuffel 1, P Gilot 1, B Limbourg 1, B Naerhuyzen 1, C Dieterich 1, M Coene 1, L Machtelinckx 1, C Cocito 1
PMCID: PMC263648  PMID: 8051246

Abstract

The previously described (M. De Kesel, P. Gilot, M.-C. Misonne, M. Coene, and C. Cocito, J. Clin. Microbiol., 31:947-954, 1993) a362 recombinant polypeptide of Mycobacterium paratuberculosis was used as reagent for an enzyme-linked immunosorbent assay (ELISA). This ELISA, which is endowed with species specificity with respect to the other mycobacteria, was applied to the analysis of bovine paratuberculosis (Johne's disease), an endemic mycobacteriosis of cattle caused by M. paratuberculosis. The distribution of anti-a362 antibodies in the cattle population was analyzed by a computer program (mixture population model) to determine a cutoff value for the test. The prevalence of a362 seropositivity in the Belgian bovine population was estimated to be 12%. The sensitivity of the a362 assay was 70%, as determined with reference sera from the U.S. National Repository of Paratuberculosis Specimens. Some 40% of the animals in the herds with paratuberculosis analyzed were found to be positive by the a362 assay. The latter proved to be 95% specific with respect to both healthy and tuberculous cattle.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bech-Nielsen S., Jorgensen J. B., Ahrens P., Feld N. C. Diagnostic accuracy of a Mycobacterium phlei-absorbed serum enzyme-linked immunosorbent assay for diagnosis of bovine paratuberculosis in dairy cows. J Clin Microbiol. 1992 Mar;30(3):613–618. doi: 10.1128/jcm.30.3.613-618.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Behymer D. E., Riemann H. P., Utterback W., D-Elmi C., Franti C. E. Mass screening of cattle sera against 14 infectious disease agents, using an ELISA system for monitoring health in livestock. Am J Vet Res. 1991 Oct;52(10):1699–1705. [PubMed] [Google Scholar]
  3. Berg Jörgenson J., Jensen P. T. Enzyme-linked immunosorbent assay (ELISA) for detection of antibodies to Mycobacterium paratuberculosis in cattle. Acta Vet Scand. 1978;19(2):310–312. doi: 10.1186/BF03547635. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Chiodini R. J., Van Kruiningen H. J., Merkal R. S. Ruminant paratuberculosis (Johne's disease): the current status and future prospects. Cornell Vet. 1984 Jul;74(3):218–262. [PubMed] [Google Scholar]
  5. Colgrove G. S., Thoen C. O., Blackburn B. O., Murphy C. D. Paratuberculosis in cattle: a comparison of three serologic tests with results of fecal culture. Vet Microbiol. 1989 Feb;19(2):183–187. doi: 10.1016/0378-1135(89)90083-7. [DOI] [PubMed] [Google Scholar]
  6. Collins M. T., Angulo A., Buergelt C. D., Hennager S. G., Hietala S. K., Jacobson R. H., Whipple D. L., Whitlock R. H. Reproducibility of a commercial enzyme-linked immunosorbent assay for bovine paratuberculosis among eight laboratories. J Vet Diagn Invest. 1993 Jan;5(1):52–55. doi: 10.1177/104063879300500112. [DOI] [PubMed] [Google Scholar]
  7. Collins M. T., Sockett D. C., Ridge S., Cox J. C. Evaluation of a commercial enzyme-linked immunosorbent assay for Johne's disease. J Clin Microbiol. 1991 Feb;29(2):272–276. doi: 10.1128/jcm.29.2.272-276.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. De Kesel M., Gilot P., Coene M., Cocito C. Composition and immunological properties of the protein fraction of A36, a major antigen complex of Mycobacterium paratuberculosis. Scand J Immunol. 1992 Aug;36(2):201–212. doi: 10.1111/j.1365-3083.1992.tb03092.x. [DOI] [PubMed] [Google Scholar]
  9. De Kesel M., Gilot P., Misonne M. C., Coene M., Cocito C. Cloning and expression of portions of the 34-kilodalton-protein gene of Mycobacterium paratuberculosis: its application to serological analysis of Johne's disease. J Clin Microbiol. 1993 Apr;31(4):947–954. doi: 10.1128/jcm.31.4.947-954.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gilmour N. J., Goudswaard J. Corynebacterium renale as a cause of reactions to the complement fixation test for Johne's disease. J Comp Pathol. 1972 Jul;82(3):333–336. doi: 10.1016/0021-9975(72)90014-x. [DOI] [PubMed] [Google Scholar]
  11. Gilot P., Cocito C. Comparative analysis of three sensitins used in cutaneous testing for tuberculosis and paratuberculosis in cattle. FEMS Microbiol Lett. 1993 Jul 1;110(3):307–311. doi: 10.1111/j.1574-6968.1993.tb06340.x. [DOI] [PubMed] [Google Scholar]
  12. Gilot P., De Kesel M., Coene M., Cocito C. Induction of cellular immune reactions by A36, an antigen complex of Mycobacterium paratuberculosis: comparison of A36 and johnin components. Scand J Immunol. 1992 Dec;36(6):811–821. doi: 10.1111/j.1365-3083.1992.tb03143.x. [DOI] [PubMed] [Google Scholar]
  13. Gilot P., De Kesel M., Machtelinckx L., Coene M., Cocito C. Isolation and sequencing of the gene coding for an antigenic 34-kilodalton protein of Mycobacterium paratuberculosis. J Bacteriol. 1993 Aug;175(15):4930–4935. doi: 10.1128/jb.175.15.4930-4935.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Körmendy B. Diagnostic value of mammalian, avian and johnin PPD tuberculins in cattle herds infected by Mycobacterium paratuberculosis. Acta Vet Hung. 1988;36(3-4):177–183. [PubMed] [Google Scholar]
  15. McKenzie R. A., Ward W. H. Rhodococcus (Corynebacterium) equi: a possible cause of reactions to the complement fixation test for Johne's disease of cattle. Aust Vet J. 1981 Apr;57(4):200–201. doi: 10.1111/j.1751-0813.1981.tb00517.x. [DOI] [PubMed] [Google Scholar]
  16. Merkal R. S., Curran B. J. Growth and metabolic characteristics of Mycobacterium paratuberculosis. Appl Microbiol. 1974 Aug;28(2):276–279. doi: 10.1128/am.28.2.276-279.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Milner A. R., Lepper A. W., Symonds W. N., Gruner E. Analysis by ELISA and Western blotting of antibody reactivities in cattle infected with Mycobacterium paratuberculosis after absorption of serum with M phlei. Res Vet Sci. 1987 Mar;42(2):140–144. [PubMed] [Google Scholar]
  18. Milner A. R., Mack W. N., Coates K. J., Hill J., Gill I., Sheldrick P. The sensitivity and specificity of a modified ELISA for the diagnosis of Johne's disease from a field trial in cattle. Vet Microbiol. 1990 Nov;25(2-3):193–198. doi: 10.1016/0378-1135(90)90076-8. [DOI] [PubMed] [Google Scholar]
  19. Parker R. A., Erdman D. D., Anderson L. J. Use of mixture models in determining laboratory criterion for identification of seropositive individuals: application to parvovirus B19 serology. J Virol Methods. 1990 Feb;27(2):135–144. doi: 10.1016/0166-0934(90)90130-8. [DOI] [PubMed] [Google Scholar]
  20. Pepin M., Marly J., Pardon P. Corynebacterium pseudotuberculosis infection in sheep and the complement fixation test for paratuberculosis. Vet Rec. 1987 Mar 7;120(10):236–236. doi: 10.1136/vr.120.10.236. [DOI] [PubMed] [Google Scholar]
  21. Poupart P., Coene M., Van Heuverswyn H., Cocito C. Preparation of a specific RNA probe for detection of Mycobacterium paratuberculosis and diagnosis of Johne's disease. J Clin Microbiol. 1993 Jun;31(6):1601–1605. doi: 10.1128/jcm.31.6.1601-1605.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Ridge S. E., Vizard A. L. Determination of the optimal cutoff value for a serological assay: an example using the Johne's Absorbed EIA. J Clin Microbiol. 1993 May;31(5):1256–1261. doi: 10.1128/jcm.31.5.1256-1261.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Sherman D. M., Markham R. J., Bates F. Agar gel immunodiffusion test for diagnosis of clinical paratuberculosis in cattle. J Am Vet Med Assoc. 1984 Jul 15;185(2):179–182. [PubMed] [Google Scholar]
  24. Sockett D. C., Carr D. J., Richards W. D., Collins M. T. A repository of specimens for comparison of diagnostic testing procedures for bovine paratuberculosis. J Vet Diagn Invest. 1992 Apr;4(2):188–191. doi: 10.1177/104063879200400213. [DOI] [PubMed] [Google Scholar]
  25. Sockett D. C., Conrad T. A., Thomas C. B., Collins M. T. Evaluation of four serological tests for bovine paratuberculosis. J Clin Microbiol. 1992 May;30(5):1134–1139. doi: 10.1128/jcm.30.5.1134-1139.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Yokomizo Y., Merkal R. S., Lyle P. A. Enzyme-linked immunosorbent assay for detection of bovine immunoglobulin G1 antibody to a protoplasmic antigen of Mycobacterium paratuberculosis. Am J Vet Res. 1983 Nov;44(11):2205–2207. [PubMed] [Google Scholar]
  27. Yokomizo Y., Yugi H., Merkal R. S. A method for avoiding false-positive reactions in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of bovine paratuberculosis. Nihon Juigaku Zasshi. 1985 Feb;47(1):111–119. doi: 10.1292/jvms1939.47.111. [DOI] [PubMed] [Google Scholar]
  28. van der Giessen J. W., Haring R. M., Vauclare E., Eger A., Haagsma J., van der Zeijst B. A. Evaluation of the abilities of three diagnostic tests based on the polymerase chain reaction to detect Mycobacterium paratuberculosis in cattle: application in a control program. J Clin Microbiol. 1992 May;30(5):1216–1219. doi: 10.1128/jcm.30.5.1216-1219.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES