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. 1994 May;32(5):1333–1337. doi: 10.1128/jcm.32.5.1333-1337.1994

VP4 typing of bovine and porcine group A rotaviruses by PCR.

V Gouvea 1, N Santos 1, M do C Timenetsky 1
PMCID: PMC263688  PMID: 8051262

Abstract

A PCR typing assay was developed to identify rotavirus P types (VP4 specificity) of bovine NCDV, UK, and B223 strains and porcine OSU and Gottfried strains. Thirty-nine human and animal strains representing all known, and some undefined, rotavirus P types were used to develop and evaluate the specificity of the method. No cross-amplification was observed. The PCR results agreed with previous characterizations by monoclonal antibodies, sequence analysis, and hybridization assays, except for the Gottfried strain, which showed a P type distinct from the human asymptomatic strains. Analysis of a small number of field specimens suggested a high level of VP4 polymorphism among porcine strains. The assay should be of value in typing field isolates and tracing interspecies infections.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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