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. 1994 May;32(5):1349–1353. doi: 10.1128/jcm.32.5.1349-1353.1994

Comparison of monoclonal antibody-based sandwich enzyme-linked immunosorbent assay and virus isolation for detection of peste des petits ruminants virus in goat tissues and secretions.

J T Saliki 1, J A House 1, C A Mebus 1, E J Dubovi 1
PMCID: PMC263698  PMID: 8051266

Abstract

A monoclonal antibody-based sandwich enzyme-linked immunosorbent assay (S-ELISA) was developed for specific detection of peste des petits ruminants virus. Compared with virus isolation in Vero cell cultures using 89 paired tissue and secretion samples from six experimentally infected goats, S-ELISA was significantly more sensitive (71.9% versus 65.2%; P < 0.05). The S-ELISA is a suitable alternative to virus isolation.

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Selected References

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