Abstract
Standard broth microdilution (with and without bovine serum albumin [BSA] supplementation), tube dilution, and agar dilution susceptibility tests were compared for determining ramoplanin MICs. With a data base of 246 clinical isolates of gram-positive bacteria from 33 U.S. sites, it was shown that (i) agar and tube dilution susceptibility tests gave essentially the same results (93.9% of the test results were within 1 doubling dilution of equivalence), (ii) broth microdilution susceptibility tests gave results up to 5 doubling dilutions higher than agar or tube assays, and (iii) this data skewing could be reversed by BSA supplementation (final concentration, 0.02%) of the broth microdilution test medium.
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- Barry A. L., Pfaller M. A., Fuchs P. C. Ramoplanin susceptibility testing criteria. J Clin Microbiol. 1993 Jul;31(7):1932–1935. doi: 10.1128/jcm.31.7.1932-1935.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ciabatti R., Kettenring J. K., Winters G., Tuan G., Zerilli L., Cavalleri B. Ramoplanin (A-16686), a new glycolipodepsipeptide antibiotic. III. Structure elucidation. J Antibiot (Tokyo) 1989 Feb;42(2):254–267. doi: 10.7164/antibiotics.42.254. [DOI] [PubMed] [Google Scholar]
- Collins L. A., Eliopoulos G. M., Wennersten C. B., Ferraro M. J., Moellering R. C., Jr In vitro activity of ramoplanin against vancomycin-resistant gram-positive organisms. Antimicrob Agents Chemother. 1993 Jun;37(6):1364–1366. doi: 10.1128/aac.37.6.1364. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jones R. N., Barry A. L. In vitro evaluation of ramoplanin (A16686 or MDL62198). A new depsipeptide complex for potential topical use. Diagn Microbiol Infect Dis. 1989 May-Jun;12(3):279–282. doi: 10.1016/0732-8893(89)90029-1. [DOI] [PubMed] [Google Scholar]
- Lawrence T., Rotstein C., Beam T. R., Jr, Gorzynski E. A., Amsterdam D. In vitro activities of ramoplanin, selected glycopeptides, fluoroquinolones, and other antibiotics against clinical bloodstream isolates of gram-positive cocci. Antimicrob Agents Chemother. 1993 Apr;37(4):896–900. doi: 10.1128/aac.37.4.896. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Scotti R., Dulworth J. K., Kenny M. T., Goldstein B. P. Effect of protein on ramoplanin broth microdilution minimum inhibitory concentrations. Diagn Microbiol Infect Dis. 1993 Oct;17(3):209–211. doi: 10.1016/0732-8893(93)90098-r. [DOI] [PubMed] [Google Scholar]