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In vivo growth assay results for 50 single-cysteine mutants within key regions of the NBD of MsbA. WT and C-less MsbA are positive controls, and an empty pET28 plasmid without the MsbA gene serves as a negative control (black bars). Dark gray bars identify 26 sites within known conserved ABC transporter motifs, as identified, and light gray bars correspond to neighboring sites. Error bars represent the standard deviation from the mean.
