Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Jul;32(7):1674–1679. doi: 10.1128/jcm.32.7.1674-1679.1994

Competitive inhibition enzyme-linked immunosorbent assay for antibody in sheep and other ruminants to a conserved epitope of malignant catarrhal fever virus.

H Li 1, D T Shen 1, D P Knowles 1, J R Gorham 1, T B Crawford 1
PMCID: PMC263759  PMID: 7523438

Abstract

Malignant catarrhal fever (MCF) is a severe, usually fatal, acute systemic disease syndrome of certain domestic and wild ruminants caused by members of the family Gammaherpesvirinae. Two distinct but closely related viruses cause clinically indistinguishable syndromes: one that is indigenous to the widebeest and the other that apparently is indigenous to domestic sheep. Neither the pathogenesis nor the epidemiology of sheep-associated MCF (SA-MCF) is understood, primarily because of a lack of adequate detection methods for the etiologic agent or antibody against it. No acceptably documented isolates of SA-MCF virus have been reported, and existing antibody assays suffer from significant cross-reactivity with other viruses. As a basis for a specific serologic assay, an attempt was made to identify an epitope conserved among all isolates of MCF viruses, by using a monoclonal antibody (MAb) produced against a previously reported U.S. isolate of MCF virus. A MAb (15-A) which bound a conserved epitope present on all four isolates of MCF virus examined was found. MAb 15-A did not react with eight common sheep and goat viruses or five common bovine viruses. Immunoprecipitation revealed that the 15-A epitope was located on the viral glycoprotein complex, with molecular masses of 115, 110, 105, 78, and 45 kDa. Sera from experimentally and naturally infected animals which yielded a similar glycoprotein complex immunoprecipitation pattern competed with MAb 15-A for its epitope. A competitive inhibition enzyme-linked immunosorbent assay (ELISA) based on MAb 15-A was therefore developed. The assay detected antibody in inapparently infected sheep and in cattle, deer, and bison with clinical MCF. Of the 149 serum samples from sheep associated with MCF outbreaks, 88 (55%) were seropositive by competitive inhibition ELISA.

Full text

PDF
1674

Images in this article

1676Image
on p.1676
1676Image
on p.1676

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson J. Use of monoclonal antibody in a blocking ELISA to detect group specific antibodies to bluetongue virus. J Immunol Methods. 1984 Nov 16;74(1):139–149. doi: 10.1016/0022-1759(84)90375-2. [DOI] [PubMed] [Google Scholar]
  2. Baxter S. I., Pow I., Bridgen A., Reid H. W. PCR detection of the sheep-associated agent of malignant catarrhal fever. Arch Virol. 1993;132(1-2):145–159. doi: 10.1007/BF01309849. [DOI] [PubMed] [Google Scholar]
  3. Bridgen A., Herring A. J., Inglis N. F., Reid H. W. Preliminary characterization of the alcelaphine herpesvirus 1 genome. J Gen Virol. 1989 May;70(Pt 5):1141–1150. doi: 10.1099/0022-1317-70-5-1141. [DOI] [PubMed] [Google Scholar]
  4. Castro A. E., Daley G. G., Zimmer M. A., Whitenack D. L., Jensen J. Malignant catarrhal fever in an Indian gaur and greater kudu: experimental transmission, isolation, and identification of a herpesvirus. Am J Vet Res. 1982 Jan;43(1):5–11. [PubMed] [Google Scholar]
  5. Deinhardt F., Falk L. A., Wolfe L. G. Simian herpesviruses. Cancer Res. 1973 Jun;33(6):1424–1426. [PubMed] [Google Scholar]
  6. Hamdy F. M., Dardiri A. H., Mebus C., Pierson R. E., Johnson D. Etiology of malignant catarrhal fever outbreak in Minnesota. Proc Annu Meet U S Anim Health Assoc. 1978;(82):248–267. [PubMed] [Google Scholar]
  7. Hoffman D., Young M. P. Malignant catarrhal fever. Aust Vet J. 1989 Dec;66(12):405–406. doi: 10.1111/j.1751-0813.1989.tb13560.x. [DOI] [PubMed] [Google Scholar]
  8. Keller R., Peitchel R., Goldman J. N., Goldman M. An IgG-Fc receptor induced in cytomegalovirus-infected human fibroblasts. J Immunol. 1976 Mar;116(3):772–777. [PubMed] [Google Scholar]
  9. Knowles D. P., Jr, Perryman L. E., Goff W. L., Miller C. D., Harrington R. D., Gorham J. R. A monoclonal antibody defines a geographically conserved surface protein epitope of Babesia equi merozoites. Infect Immun. 1991 Jul;59(7):2412–2417. doi: 10.1128/iai.59.7.2412-2417.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Li H., Shen D. T., Burger D., Davis W. C., Gorham J. R. Analysis of bovine herpesvirus 4 (DN 599) major antigens with monoclonal antibodies and polyclonal immune serum. Arch Virol. 1991;119(3-4):225–238. doi: 10.1007/BF01310672. [DOI] [PubMed] [Google Scholar]
  11. Metzler A. E. The malignant catarrhal fever complex. Comp Immunol Microbiol Infect Dis. 1991;14(2):107–124. doi: 10.1016/0147-9571(91)90125-w. [DOI] [PubMed] [Google Scholar]
  12. Muluneh A., Liebermann H. Studies concerning etiology of sheep-associated malignant catarrhal fever in Europe. Dtsch Tierarztl Wochenschr. 1992 Sep;99(9):384–386. [PubMed] [Google Scholar]
  13. Mushi E. Z., Jessett D. M., Rurangirwa F. R., Rossiter P. B., Karstad L. Neutralising antibodies to malignant catarrhal fever herpesvirus in wildebeest nasal secretions. Trop Anim Health Prod. 1981 Feb;13(1):55–56. doi: 10.1007/BF02237890. [DOI] [PubMed] [Google Scholar]
  14. Mushi E. Z., Karstad L., Jessett D. M. Isolation of bovine malignant catarrhal fever virus from ocular and nasal secretions of wildebeest calves. Res Vet Sci. 1980 Sep;29(2):168–171. [PubMed] [Google Scholar]
  15. Mushi E. Z., Rurangirwa F. R. Malignant catarrhal fever virus infectivity in rabbit macrophages and monocytes. Vet Res Commun. 1981 Sep;5(1):51–56. doi: 10.1007/BF02214967. [DOI] [PubMed] [Google Scholar]
  16. PLOWRIGHT W., FERRIS R. D., SCOTT G. R. Blue wildebeest and the aetiological agent of bovine malignant catarrhal fever. Nature. 1960 Dec 31;188:1167–1169. doi: 10.1038/1881167a0. [DOI] [PubMed] [Google Scholar]
  17. PLOWRIGHT W., MACADAM R. F., ARMSTRONG J. A. GROWTH AND CHARACTERIZATION OF THE VIRUS OF BOVINE MALIGNANT CATARRHAL FEVER IN EAST AFRICA. J Gen Microbiol. 1965 May;39:253–266. doi: 10.1099/00221287-39-2-253. [DOI] [PubMed] [Google Scholar]
  18. Pierson R. E., Thake D., McChesney A. E., Storz J. An epizootic of malignant catarrhal fever in feedlot cattle. J Am Vet Med Assoc. 1973 Aug 15;163(4):349–350. [PubMed] [Google Scholar]
  19. Rahman A. A., Teschner M., Sethi K. K., Brandis H. Appearance of IgG (Fc) receptor(s) on cultured human fibroblasts infected with human cytomegalovirus. J Immunol. 1976 Jul;117(1):253–258. [PubMed] [Google Scholar]
  20. Reid H. W., Buxton D., Corrigall W., Hunter A. R., McMartin D. A., Rushton R. An outbreak of malignant catarrhal fever in red deer (Cervus elephus). Vet Rec. 1979 Feb 10;104(6):120–123. doi: 10.1136/vr.104.6.120. [DOI] [PubMed] [Google Scholar]
  21. Roizman B., Carmichael L. E., Deinhardt F., de-The G., Nahmias A. J., Plowright W., Rapp F., Sheldrick P., Takahashi M., Wolf K. Herpesviridae. Definition, provisional nomenclature, and taxonomy. The Herpesvirus Study Group, the International Committee on Taxonomy of Viruses. Intervirology. 1981;16(4):201–217. doi: 10.1159/000149269. [DOI] [PubMed] [Google Scholar]
  22. Rossiter P. B. Antibodies to malignant catarrhal fever virus in sheep sera. J Comp Pathol. 1981 Apr;91(2):303–311. doi: 10.1016/0021-9975(81)90036-0. [DOI] [PubMed] [Google Scholar]
  23. Schuller W., Cerny-Reiterer S., Silber R. Evidence that the sheep associated form of malignant catarrhal fever is caused by a herpes virus. Zentralbl Veterinarmed B. 1990 Aug;37(6):442–447. doi: 10.1111/j.1439-0450.1990.tb01081.x. [DOI] [PubMed] [Google Scholar]
  24. Shek W. R., Calnek B. W., Schat K. A., Chen C. H. Characterization of Marek's disease virus-infected lymphocytes: discrimination between cytolytically and latently infected cells. J Natl Cancer Inst. 1983 Mar;70(3):485–491. [PubMed] [Google Scholar]
  25. WATKINS J. F. ADSORPTION OF SENSITIZED SHEEP ERYTHROCYTES TO HELA CELLS INFECTED WITH HERPES SIMPLEX VIRUS. Nature. 1964 Jun 27;202:1364–1365. doi: 10.1038/2021364a0. [DOI] [PubMed] [Google Scholar]
  26. Westmoreland D., Watkins J. F. The IgG receptor induced by herpes simplex virus: studies using radioiodinated IgG. J Gen Virol. 1974 Jul;24(1):167–178. doi: 10.1099/0022-1317-24-1-167. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES