Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Jul;32(7):1750–1756. doi: 10.1128/jcm.32.7.1750-1756.1994

Development of o.a.s.i.s., a new automated blood culture system in which detection is based on measurement of bottle headspace pressure changes.

C M Stevens 1, D Swaine 1, C Butler 1, A H Carr 1, A Weightman 1, C R Catchpole 1, D E Healing 1, T S Elliott 1
PMCID: PMC263784  PMID: 7929769

Abstract

o.a.s.i.s. (Unipath Ltd., Basingstoke, United Kingdom) is a new automated blood culture system. The metabolism of microorganisms is detected by measuring changes in the pressure of the headspace of blood culture bottles. These changes are measured by monitoring the position of a flexible sealing septum, every 5 min, with a scanning laser sensor. This noninvasive system can detect both gas absorption and production and does not rely solely on measuring increasing carbon dioxide levels. A research prototype instrument was used to carry out an evaluation of the media, the detection system, and its associated detection algorithm. In simulated blood cultures, o.a.s.i.s. supported growth and detected a range of clinical isolates. Times to positivity were significantly shorter in o.a.s.i.s. than in the BACTEC 460 system. Results of a clinical feasibility study, with a manual blood culture system as a control, confirmed that o.a.s.i.s. was able to support the growth and detection of a variety of clinically significant organisms. On the basis of these findings, full-scale comparative clinical trials of o.a.s.i.s. with other automated blood culture systems are warranted.

Full text

PDF
1750

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Appelbaum P. C. World-wide development of antibiotic resistance in pneumococci. Eur J Clin Microbiol. 1987 Aug;6(4):367–377. doi: 10.1007/BF02013089. [DOI] [PubMed] [Google Scholar]
  2. Courcol R. J., Duhamel M., Decoster A., Lemaire V. M., Rastorgoueff M. L., Ochin D., Martin G. R. BioArgos: a fully automated blood culture system. J Clin Microbiol. 1992 Aug;30(8):1995–1998. doi: 10.1128/jcm.30.8.1995-1998.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Elliott T. S., Catchpole C. R., Healing D. E. Blood for culture. Lancet. 1993 Dec 18;342(8886-8887):1557–1557. doi: 10.1016/s0140-6736(05)80131-x. [DOI] [PubMed] [Google Scholar]
  4. Eng J., Holten E. Gelatin neutralization of the inhibitory effect of sodium polyanethol sulfonate on Neisseria meningitidis in blood culture media. J Clin Microbiol. 1977 Jul;6(1):1–3. doi: 10.1128/jcm.6.1.1-3.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Morello J. A., Leitch C., Nitz S., Dyke J. W., Andruszewski M., Maier G., Landau W., Beard M. A. Detection of bacteremia by Difco ESP blood culture system. J Clin Microbiol. 1994 Mar;32(3):811–818. doi: 10.1128/jcm.32.3.811-818.1994. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nolte F. S., Williams J. M., Jerris R. C., Morello J. A., Leitch C. D., Matushek S., Schwabe L. D., Dorigan F., Kocka F. E. Multicenter clinical evaluation of a continuous monitoring blood culture system using fluorescent-sensor technology (BACTEC 9240). J Clin Microbiol. 1993 Mar;31(3):552–557. doi: 10.1128/jcm.31.3.552-557.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Sawhney D., Hinder S., Swaine D., Bridson E. Y. Novel method for detecting micro-organisms in blood cultures. J Clin Pathol. 1986 Nov;39(11):1259–1263. doi: 10.1136/jcp.39.11.1259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Stevens M., Patel H., Walters A., Burch K., Jay A., Dowling N., Mitchell C. J., Swann R. A., Willis A. T., Shanson D. C. Comparison of Sentinel and Bactec blood culture systems. J Clin Pathol. 1992 Sep;45(9):815–818. doi: 10.1136/jcp.45.9.815. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Thorpe T. C., Wilson M. L., Turner J. E., DiGuiseppi J. L., Willert M., Mirrett S., Reller L. B. BacT/Alert: an automated colorimetric microbial detection system. J Clin Microbiol. 1990 Jul;28(7):1608–1612. doi: 10.1128/jcm.28.7.1608-1612.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Washington J. A. Blood cultures: an overview. Eur J Clin Microbiol Infect Dis. 1989 Sep;8(9):803–806. doi: 10.1007/BF02185852. [DOI] [PubMed] [Google Scholar]
  11. Waters J. R. Detection of bacterial growth by gas absorption. J Clin Microbiol. 1992 May;30(5):1205–1209. doi: 10.1128/jcm.30.5.1205-1209.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES