Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1994 Jul;32(7):1763–1767. doi: 10.1128/jcm.32.7.1763-1767.1994

Restriction fragment length polymorphism analysis of clinical isolates of Mycobacterium haemophilum.

K Kikuchi 1, E M Bernard 1, T E Kiehn 1, D Armstrong 1, L W Riley 1
PMCID: PMC263787  PMID: 7929771

Abstract

Mycobacterium haemophilum is an emerging opportunistic pathogen, and since 1989, infections caused by this organism have been identified more frequently in the New York City area than in any other region of the United States. A DNA fingerprinting method, based on restriction fragment length polymorphisms (RFLPs) was developed. A genomic library of M. haemophilum isolate 1A was constructed; screening the library yielded a recombinant strain that incorporated a genetic element present in multiple copies in the M. haemophilum genome. This clone was used to produce a probe for RFLP analyses of PvuII digests of genomic DNA. We used this probe to determine the RFLP patterns of 43 clinical isolates of M. haemophilum from 28 patients. A total of six distinct patterns were observed. Two patterns, designated types 1 and 2, accounted for 91% of the infections in patients from the New York City area. Two isolates from Arizona had identical patterns but were distinct from those of New York isolates, and an isolate from Israel, the type strain, had another distinct pattern (type 6). The type 6 pattern was also seen in a recent isolate from Norway. All of the type 1 isolates and 60% of the type 2 isolates were recovered from patients with AIDS in the New York City area. This molecular subtyping method should provide a useful tool for epidemiological studies and may help identify the associated risk factors, vehicles, and possible reservoirs of this newly emerging pathogen.

Full text

PDF
1763

Images in this article

1765Image
on p.1765
1766Image
on p.1766

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Becherer P., Hopfer R. L. Infection with Mycobacterium haemophilum. Clin Infect Dis. 1992 Mar;14(3):793–793. doi: 10.1093/clinids/14.3.793. [DOI] [PubMed] [Google Scholar]
  2. Bernard E. M., Edwards F. F., Kiehn T. E., Brown S. T., Armstrong D. Activities of antimicrobial agents against clinical isolates of Mycobacterium haemophilum. Antimicrob Agents Chemother. 1993 Nov;37(11):2323–2326. doi: 10.1128/aac.37.11.2323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dawson D. J., Blacklock Z. M., Kane D. W. Mycobacterium haemophilum causing lymphadenitis in an otherwise healthy child. Med J Aust. 1981 Sep 19;2(6):289–290. doi: 10.5694/j.1326-5377.1981.tb128321.x. [DOI] [PubMed] [Google Scholar]
  4. Dawson D. J., Jennis F. Mycobacteria with a growth requirement for ferric ammonium citrate, identified as Mycobacterium haemophilum. J Clin Microbiol. 1980 Feb;11(2):190–192. doi: 10.1128/jcm.11.2.190-192.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dever L. L., Martin J. W., Seaworth B., Jorgensen J. H. Varied presentations and responses to treatment of infections caused by Mycobacterium haemophilum in patients with AIDS. Clin Infect Dis. 1992 Jun;14(6):1195–1200. doi: 10.1093/clinids/14.6.1195. [DOI] [PubMed] [Google Scholar]
  6. Holton J., Nye P., Miller R. Mycobacterium haemophilum infection in a patient with AIDS. J Infect. 1991 Nov;23(3):303–306. doi: 10.1016/0163-4453(91)93080-v. [DOI] [PubMed] [Google Scholar]
  7. Kiehn T. E., White M., Pursell K. J., Boone N., Tsivitis M., Brown A. E., Polsky B., Armstrong D. A cluster of four cases of Mycobacterium haemophilum infection. Eur J Clin Microbiol Infect Dis. 1993 Feb;12(2):114–118. doi: 10.1007/BF01967586. [DOI] [PubMed] [Google Scholar]
  8. Kristjansson M., Bieluch V. M., Byeff P. D. Mycobacterium haemophilum infection in immunocompromised patients: case report and review of the literature. Rev Infect Dis. 1991 Sep-Oct;13(5):906–910. doi: 10.1093/clinids/13.5.906. [DOI] [PubMed] [Google Scholar]
  9. Mezo A., Jennis F., McCarthy S. W., Dawson D. J. Unusual mycobacteria in 5 cases of opportunistic infections. Pathology. 1979 Jul;11(3):377–384. doi: 10.3109/00313027909059014. [DOI] [PubMed] [Google Scholar]
  10. Sanger F., Nicklen S., Coulson A. R. DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463–5467. doi: 10.1073/pnas.74.12.5463. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Small P. M., Shafer R. W., Hopewell P. C., Singh S. P., Murphy M. J., Desmond E., Sierra M. F., Schoolnik G. K. Exogenous reinfection with multidrug-resistant Mycobacterium tuberculosis in patients with advanced HIV infection. N Engl J Med. 1993 Apr 22;328(16):1137–1144. doi: 10.1056/NEJM199304223281601. [DOI] [PubMed] [Google Scholar]
  12. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  13. Straus W. L., Ostroff S. M., Jernigan D. B., Kiehn T. E., Sordillo E. M., Armstrong D., Boone N., Schneider N., Kilburn J. O., Silcox V. A. Clinical and epidemiologic characteristics of Mycobacterium haemophilum, an emerging pathogen in immunocompromised patients. Ann Intern Med. 1994 Jan 15;120(2):118–125. doi: 10.7326/0003-4819-120-2-199401150-00004. [DOI] [PubMed] [Google Scholar]
  14. Thibert L., Lebel F., Martineau B. Two cases of Mycobacterium haemophilum infection in Canada. J Clin Microbiol. 1990 Mar;28(3):621–623. doi: 10.1128/jcm.28.3.621-623.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Yarrish R. L., Shay W., LaBombardi V. J., Meyerson M., Miller D. K., Larone D. Osteomyelitis caused by Mycobacterium haemophilum: successful therapy in two patients with AIDS. AIDS. 1992 Jun;6(6):557–561. [PubMed] [Google Scholar]
  16. van Embden J. D., Cave M. D., Crawford J. T., Dale J. W., Eisenach K. D., Gicquel B., Hermans P., Martin C., McAdam R., Shinnick T. M. Strain identification of Mycobacterium tuberculosis by DNA fingerprinting: recommendations for a standardized methodology. J Clin Microbiol. 1993 Feb;31(2):406–409. doi: 10.1128/jcm.31.2.406-409.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES