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. 1994 Jul;32(7):1799–1804. doi: 10.1128/jcm.32.7.1799-1804.1994

Characterization and presumptive identification of Helicobacter pylori isolates from rhesus monkeys.

E S Drazek 1, A Dubois 1, R K Holmes 1
PMCID: PMC263799  PMID: 7523441

Abstract

We characterized 38 Helicobacter isolates, including 22 from gastric biopsy samples obtained from 14 rhesus monkeys and single isolates from 16 monkeys in a different colony. Biochemical profiles of these isolates were nearly identical to that of Helicobacter pylori ATCC 43504. Restriction fragment length polymorphism (RFLP) analysis indicated that each infected monkey harbored one to four strains. The 17 RFLP types found among these 22 isolates differed from all seven RFLPs found among the other 16 isolates. Thus, monkeys within a given colony are more likely to be infected by Helicobacter isolates with the same or a similar RFLP than are monkeys from different colonies. A 16S rRNA gene was amplified by PCR and cloned from the Helicobacter isolate from rhesus monkey 85D08. Ribotyping with this probe demonstrated less diversity among isolates from rhesus monkeys than was reported among isolates of H. pylori from humans, as did RFLP analysis of a PCR fragment of the ureA-ureB gene cluster. The DNA sequence of the cloned 16S rRNA gene was determined and compared with sequences reported for H. pylori and other Helicobacter species. Our analysis of 127 nucleotides (corresponding with residues 1240 to 1366 of the Escherichia coli 16S rRNA gene) indicated that the Helicobacter isolate from monkey 85D08 was 99.2 to 100% homologous to isolates of H. pylori from humans but only 83.5 to 96.9% homologous with other Helicobacter species in this region of the 16S rRNA gene. These data provide strong support for the presumptive identification of these isolates as H. pylori.

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Selected References

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  1. Blaser M. J. Epidemiology and pathophysiology of Campylobacter pylori infections. Rev Infect Dis. 1990 Jan-Feb;12 (Suppl 1):S99–106. doi: 10.1093/clinids/12.supplement_1.s99. [DOI] [PubMed] [Google Scholar]
  2. Bronsdon M. A., Goodwin C. S., Sly L. I., Chilvers T., Schoenknecht F. D. Helicobacter nemestrinae sp. nov., a spiral bacterium found in the stomach of a pigtailed macaque (Macaca nemestrina) Int J Syst Bacteriol. 1991 Jan;41(1):148–153. doi: 10.1099/00207713-41-1-148. [DOI] [PubMed] [Google Scholar]
  3. Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Dubois A., Fiala N., Heman-Ackah L. M., Drazek E. S., Tarnawski A., Fishbein W. N., Perez-Perez G. I., Blaser M. J. Natural gastric infection with Helicobacter pylori in monkeys: a model for spiral bacteria infection in humans. Gastroenterology. 1994 Jun;106(6):1405–1417. doi: 10.1016/0016-5085(94)90392-1. [DOI] [PubMed] [Google Scholar]
  5. Dubois A., Tarnawski A., Newell D. G., Fiala N., Dabros W., Stachura J., Krivan H., Heman-Ackah L. M. Gastric injury and invasion of parietal cells by spiral bacteria in rhesus monkeys. Are gastritis and hyperchlorhydria infectious diseases? Gastroenterology. 1991 Apr;100(4):884–891. doi: 10.1016/0016-5085(91)90260-r. [DOI] [PubMed] [Google Scholar]
  6. Eaton K. A., Brooks C. L., Morgan D. R., Krakowka S. Essential role of urease in pathogenesis of gastritis induced by Helicobacter pylori in gnotobiotic piglets. Infect Immun. 1991 Jul;59(7):2470–2475. doi: 10.1128/iai.59.7.2470-2475.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Euler A. R., Zurenko G. E., Moe J. B., Ulrich R. G., Yagi Y. Evaluation of two monkey species (Macaca mulatta and Macaca fascicularis) as possible models for human Helicobacter pylori disease. J Clin Microbiol. 1990 Oct;28(10):2285–2290. doi: 10.1128/jcm.28.10.2285-2290.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Foxall P. A., Hu L. T., Mobley H. L. Use of polymerase chain reaction-amplified Helicobacter pylori urease structural genes for differentiation of isolates. J Clin Microbiol. 1992 Mar;30(3):739–741. doi: 10.1128/jcm.30.3.739-741.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hazell S. L., Eichberg J. W., Lee D. R., Alpert L., Evans D. G., Evans D. J., Jr, Graham D. Y. Selection of the chimpanzee over the baboon as a model for Helicobacter pylori infection. Gastroenterology. 1992 Sep;103(3):848–854. doi: 10.1016/0016-5085(92)90016-r. [DOI] [PubMed] [Google Scholar]
  10. Ho S. A., Hoyle J. A., Lewis F. A., Secker A. D., Cross D., Mapstone N. P., Dixon M. F., Wyatt J. I., Tompkins D. S., Taylor G. R. Direct polymerase chain reaction test for detection of Helicobacter pylori in humans and animals. J Clin Microbiol. 1991 Nov;29(11):2543–2549. doi: 10.1128/jcm.29.11.2543-2549.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Krakowka S., Morgan D. R., Kraft W. G., Leunk R. D. Establishment of gastric Campylobacter pylori infection in the neonatal gnotobiotic piglet. Infect Immun. 1987 Nov;55(11):2789–2796. doi: 10.1128/iai.55.11.2789-2796.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Majewski S. I., Goodwin C. S. Restriction endonuclease analysis of the genome of Campylobacter pylori with a rapid extraction method: evidence for considerable genomic variation. J Infect Dis. 1988 Mar;157(3):465–471. doi: 10.1093/infdis/157.3.465. [DOI] [PubMed] [Google Scholar]
  13. McNulty C. A., Dent J. C. Rapid identification of Campylobacter pylori (C. pyloridis) by preformed enzymes. J Clin Microbiol. 1987 Sep;25(9):1683–1686. doi: 10.1128/jcm.25.9.1683-1686.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Morgan D. D., Owen R. J. Use of DNA restriction endonuclease digest and ribosomal RNA gene probe patterns to fingerprint Helicobacter pylori and Helicobacter mustelae isolated from human and animal hosts. Mol Cell Probes. 1990 Aug;4(4):321–334. doi: 10.1016/0890-8508(90)90023-s. [DOI] [PubMed] [Google Scholar]
  15. Newell D. G., Hudson M. J., Baskerville A. Naturally occurring gastritis associated with Campylobacter pylori infection in the rhesus monkey. Lancet. 1987 Dec 5;2(8571):1338–1338. doi: 10.1016/s0140-6736(87)91236-0. [DOI] [PubMed] [Google Scholar]
  16. Romaniuk P. J., Zoltowska B., Trust T. J., Lane D. J., Olsen G. J., Pace N. R., Stahl D. A. Campylobacter pylori, the spiral bacterium associated with human gastritis, is not a true Campylobacter sp. J Bacteriol. 1987 May;169(5):2137–2141. doi: 10.1128/jb.169.5.2137-2141.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Salvi R. J., Ahroon W., Saunders S. S., Arnold S. A. Evoked potentials: computer-automated threshold-tracking procedure using an objective detection criterion. Ear Hear. 1987 Jun;8(3):151–156. [PubMed] [Google Scholar]
  18. Simor A. E., Shames B., Drumm B., Sherman P., Low D. E., Penner J. L. Typing of Campylobacter pylori by bacterial DNA restriction endonuclease analysis and determination of plasmid profile. J Clin Microbiol. 1990 Jan;28(1):83–86. doi: 10.1128/jcm.28.1.83-86.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Solnick J. V., O'Rourke J., Lee A., Paster B. J., Dewhirst F. E., Tompkins L. S. An uncultured gastric spiral organism is a newly identified Helicobacter in humans. J Infect Dis. 1993 Aug;168(2):379–385. doi: 10.1093/infdis/168.2.379. [DOI] [PubMed] [Google Scholar]
  20. Stemmer W. P. A 20-minute ethidium bromide/high-salt extraction protocol for plasmid DNA. Biotechniques. 1991 Jun;10(6):726–726. [PubMed] [Google Scholar]
  21. Talley N. J., Zinsmeister A. R., Weaver A., DiMagno E. P., Carpenter H. A., Perez-Perez G. I., Blaser M. J. Gastric adenocarcinoma and Helicobacter pylori infection. J Natl Cancer Inst. 1991 Dec 4;83(23):1734–1739. doi: 10.1093/jnci/83.23.1734. [DOI] [PubMed] [Google Scholar]
  22. Taylor D. E., Eaton M., Chang N., Salama S. M. Construction of a Helicobacter pylori genome map and demonstration of diversity at the genome level. J Bacteriol. 1992 Nov;174(21):6800–6806. doi: 10.1128/jb.174.21.6800-6806.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Tee W., Lambert J., Smallwood R., Schembri M., Ross B. C., Dwyer B. Ribotyping of Helicobacter pylori from clinical specimens. J Clin Microbiol. 1992 Jun;30(6):1562–1567. doi: 10.1128/jcm.30.6.1562-1567.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Thillainayagam A. V., Arvind A. S., Cook R. S., Harrison I. G., Tabaqchali S., Farthing M. J. Diagnostic efficiency of an ultrarapid endoscopy room test for Helicobacter pylori. Gut. 1991 May;32(5):467–469. doi: 10.1136/gut.32.5.467. [DOI] [PMC free article] [PubMed] [Google Scholar]

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