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. 1994 Aug;32(8):1945–1948. doi: 10.1128/jcm.32.8.1945-1948.1994

Typing of group A streptococci by random amplified polymorphic DNA analysis.

H Seppälä 1, Q He 1, M Osterblad 1, P Huovinen 1
PMCID: PMC263908  PMID: 7989547

Abstract

Random amplified polymorphic DNA (RAPD) analysis was evaluated in comparison with restriction endonuclease analysis (REA) of genomic DNA and serotyping in the typing of 160 epidemiologically unrelated group A streptococci (GAS). Amplification of genomic DNA of GAS was performed with a single primer with an arbitrarily selected nucleotide sequence of 12 nucleotides. In total, 31 RAPD patterns and 15 REA patterns were observed among the isolates studied. The results of RAPD analysis were in accordance with the results of REA for 86% of the isolates, as both methods identified 15 different strains among 138 isolates. However, RAPD analysis differentiated 16 additional strains among 22 isolates. RAPD analysis was somewhat better than REA for differentiation of isolates of the same and different serotypes. However, not all of the serotypes were differentiated by RAPD analysis either. In conclusion, RAPD analysis provides a practical alternative for genomic typing of GAS. It can be recommended for the typing of GAS, especially if used in parallel with serotyping.

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Selected References

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  1. Bingen E., Denamur E., Lambert-Zechovsky N., Boissinot C., Brahimi N., Aujard Y., Blot P., Elion J. Mother-to-infant vertical transmission and cross-colonization of Streptococcus pyogenes confirmed by DNA restriction fragment length polymorphism analysis. J Infect Dis. 1992 Jan;165(1):147–150. doi: 10.1093/infdis/165.1.147. [DOI] [PubMed] [Google Scholar]
  2. Bingen E., Denamur E., Lambert-Zechovsky N., Braimi N., el Lakany M., Elion J. DNA restriction fragment length polymorphism differentiates recurrence from relapse in treatment failures of Streptococcus pyogenes pharyngitis. J Med Microbiol. 1992 Sep;37(3):162–164. doi: 10.1099/00222615-37-3-162. [DOI] [PubMed] [Google Scholar]
  3. Bisno A. L. Group A streptococcal infections and acute rheumatic fever. N Engl J Med. 1991 Sep 12;325(11):783–793. doi: 10.1056/NEJM199109123251106. [DOI] [PubMed] [Google Scholar]
  4. Bostock A., Khattak M. N., Matthews R., Burnie J. Comparison of PCR fingerprinting, by random amplification of polymorphic DNA, with other molecular typing methods for Candida albicans. J Gen Microbiol. 1993 Sep;139(9):2179–2184. doi: 10.1099/00221287-139-9-2179. [DOI] [PubMed] [Google Scholar]
  5. Cleary P. P., Kaplan E. L., Handley J. P., Wlazlo A., Kim M. H., Hauser A. R., Schlievert P. M. Clonal basis for resurgence of serious Streptococcus pyogenes disease in the 1980s. Lancet. 1992 Feb 29;339(8792):518–521. doi: 10.1016/0140-6736(92)90339-5. [DOI] [PubMed] [Google Scholar]
  6. Cleary P. P., Kaplan E. L., Livdahl C., Skjold S. DNA fingerprints of Streptococcus pyogenes are M type specific. J Infect Dis. 1988 Dec;158(6):1317–1323. doi: 10.1093/infdis/158.6.1317. [DOI] [PubMed] [Google Scholar]
  7. Ellsworth D. L., Rittenhouse K. D., Honeycutt R. L. Artifactual variation in randomly amplified polymorphic DNA banding patterns. Biotechniques. 1993 Feb;14(2):214–217. [PubMed] [Google Scholar]
  8. Fischetti V. A. Streptococcal M protein: molecular design and biological behavior. Clin Microbiol Rev. 1989 Jul;2(3):285–314. doi: 10.1128/cmr.2.3.285. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. He Q., Viljanen M. K., Mertsola J. Effects of thermocyclers and primers on the reproducibility of banding patterns in randomly amplified polymorphic DNA analysis. Mol Cell Probes. 1994 Apr;8(2):155–159. doi: 10.1006/mcpr.1994.1021. [DOI] [PubMed] [Google Scholar]
  10. Jayarao B. M., Bassam B. J., Caetano-Anollés G., Gresshoff P. M., Oliver S. P. Subtyping of Streptococcus uberis by DNA amplification fingerprinting. J Clin Microbiol. 1992 May;30(5):1347–1350. doi: 10.1128/jcm.30.5.1347-1350.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lopez E. L., Diaz M., Devoto S., Grinstein S., Woloj M., Murray B. E., Rubeglio E., Mendilaharzu F., Turco M., Vasquez M. Evidence of infection with organisms producing Shiga-like toxins in household contacts of children with the hemolytic uremic syndrome. Pediatr Infect Dis J. 1991 Jan;10(1):20–24. doi: 10.1097/00006454-199101000-00005. [DOI] [PubMed] [Google Scholar]
  12. MacPherson J. M., Eckstein P. E., Scoles G. J., Gajadhar A. A. Variability of the random amplified polymorphic DNA assay among thermal cyclers, and effects of primer and DNA concentration. Mol Cell Probes. 1993 Aug;7(4):293–299. doi: 10.1006/mcpr.1993.1043. [DOI] [PubMed] [Google Scholar]
  13. Martin D. R., Single L. A. Molecular epidemiology of group A streptococcus M type 1 infections. J Infect Dis. 1993 May;167(5):1112–1117. doi: 10.1093/infdis/167.5.1112. [DOI] [PubMed] [Google Scholar]
  14. Micheli M. R., Bova R., Calissano P., D'Ambrosio E. Randomly amplified polymorphic DNA fingerprinting using combinations of oligonucleotide primers. Biotechniques. 1993 Sep;15(3):388–390. [PubMed] [Google Scholar]
  15. Penner G. A., Bush A., Wise R., Kim W., Domier L., Kasha K., Laroche A., Scoles G., Molnar S. J., Fedak G. Reproducibility of random amplified polymorphic DNA (RAPD) analysis among laboratories. PCR Methods Appl. 1993 May;2(4):341–345. doi: 10.1101/gr.2.4.341. [DOI] [PubMed] [Google Scholar]
  16. Seppälä H., Nissinen A., Järvinen H., Huovinen S., Henriksson T., Herva E., Holm S. E., Jahkola M., Katila M. L., Klaukka T. Resistance to erythromycin in group A streptococci. N Engl J Med. 1992 Jan 30;326(5):292–297. doi: 10.1056/NEJM199201303260503. [DOI] [PubMed] [Google Scholar]
  17. Seppälä H., Vuopio-Varkila J., Osterblad M., Jahkola M., Rummukainen M., Holm S. E., Huovinen P. Evaluation of methods for epidemiologic typing of group A streptococci. J Infect Dis. 1994 Mar;169(3):519–525. doi: 10.1093/infdis/169.3.519. [DOI] [PubMed] [Google Scholar]
  18. Van Steenbergen T. J., Menard C., Tijhof C. J., Mouton C., De Graaff J. Comparison of three molecular typing methods in studies of transmission of Porphyromonas gingivalis. J Med Microbiol. 1993 Dec;39(6):416–421. doi: 10.1099/00222615-39-6-416. [DOI] [PubMed] [Google Scholar]
  19. Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 1990 Dec 25;18(24):7213–7218. doi: 10.1093/nar/18.24.7213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Welsh J., McClelland M. Genomic fingerprinting using arbitrarily primed PCR and a matrix of pairwise combinations of primers. Nucleic Acids Res. 1991 Oct 11;19(19):5275–5279. doi: 10.1093/nar/19.19.5275. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Williams J. G., Kubelik A. R., Livak K. J., Rafalski J. A., Tingey S. V. DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res. 1990 Nov 25;18(22):6531–6535. doi: 10.1093/nar/18.22.6531. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. van Belkum A., Struelens M., Quint W. Typing of Legionella pneumophila strains by polymerase chain reaction-mediated DNA fingerprinting. J Clin Microbiol. 1993 Aug;31(8):2198–2200. doi: 10.1128/jcm.31.8.2198-2200.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]

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